Aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz enzimlerinin protein düzeylerine ve arilesteraz aktivitesine etkisi
Amaç: Bu çalışmanın amacı, aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz-1, paraoksonaz-2 ve paraoksonaz-3 protein düzeylerine ve arilesteraz aktivitesine etkisini araştırmaktır. Gereç ve Yöntem: HepG2 hücreleri kontrol, 0.25 mM aspirin, 0.5 mM aspirin ve 1 mM aspirin olmak üzere 4 gruba ayrıldı ve hücreler 48 saat boyunca sırasıyla 0, 0.25, 0.5 ve 1 mM aspirin ile inkübe edildi. Hücre canlılığı 3-(4,5-Dimetil-2-tiazolil)-2,5-difenil-2H-tetrazolium bromür testi ile ölçüldü. Paraoksonaz-1, paraoksonaz-2 ve paraoksonaz-3 protein düzeyleri western blot yöntemiyle ölçüldü. Arilesteraz aktivitesi substrat olarak fenilasetat kullanılarak spektrofotometrik olarak ölçüldü. Bulgular: 0.5 mM ve 1 mM aspirin hücre canlılığında anlamlı azalmaya yol açtı. Aspirin konsantrasyonları paraoksonaz-1 ve paraoksonaz-2 protein düzeylerini anlamlı olarak değiştirmedi. 0.5 ve 1 mM aspirin paraoksonaz-3 protein düzeylerini anlamlı olarak arttırdı. 0.25 mM, 0.5 mM ve 1 mM aspirin, arilesteraz aktivitesini anlamlı olarak arttırdı. Sonuç: Çalışmamız aspirinin insan kaynaklı hepatoma hücrelerinde paraoksonaz-1 ve paraoksonaz-2 protein düzeylerini değiştirmediğini, paraoksonaz-3 protein düzeylerini ve arilesteraz aktivitesini arttırdığını gösterdi.
Effect of aspirin on protein levels of paraoxonase enzymes and arylesterase activity in human-derived hepatoma cells
Aim: The aim of this study is to investigate the effect of aspirin on paraoxonase-1, paraoxonase-2 and paraoxonase-3 protein levels and arylesterase activity in human-derived hepatoma cells. Materials and Methods: HepG2 cells were divided into four groups: control, 0.25 mM aspirin, 0.5 mM aspirin and 1 mM aspirin and cells were incubated with 0, 0.25, 0.5 and 1 mM aspirin, respectively. Cell viability was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Paraoxonase-1, paraoxonase-2 and paraoxonase-3 protein levels were measured by western blotting. Arylesterase activity was measured spectrophotometrically by using phenylacetate as substrate. Results: 0.5 mM and 1 mM aspirin caused a significant decrease on cell viability. Aspirin concentrations did not significantly change paraoxonase-1 and paraoxonase-2 protein levels. 0.5 mM and 1 mM aspirin significantly increased paraoxonase-3 protein levels. 0.25 mM, 0.5 mM and 1 mM aspirin significantly increased arylesterase activity. Conclusion: Our study showed that aspirin does not change paraoxonase-1 and paraoxonase-2 protein levels but increases paraoxonase-3 protein levels and arylesterase activity in human-derived hepatoma cells.
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