T. ÖZ, Cengiz ÇZVUŞOĞLU, E. YAYGIN, Ö. AYDOĞAN, M. KORKMAZ, F. BACAKOĞLU

Akciğer tüberkülozunda Mycobacterium tuberculosis antijen fraksiyonlarına karşı serolojik yanıtın western blot yöntemiyle değerlendirilmesi

Amaç: Bu çalışmada; aktif akciğer TB'li hastalarda sonikasyonla elde edilen M.tuberculosis antijen fraksiyonlarına karşı serolojik yanıtın Western blot yöntemiyle araştırılması amaçlanmıştır. Yöntem ve Gereç: Çalışmaya Eylül 2003-Eylül 2004 tarihleri arasında Ege Üniversitesi Tıp Fakültesi Göğüs Hastalıkları Anabilim Dalı'nda izlenen 34'ü aktif akciğer TB'li hasta, 29'u kontrol grubu olmak üzere toplam 63 birey alındı. Kontrol grubu; 12'si inaktif akciğer TB, 14'ü TCT pozitif sağlıklı, 3'ü ise TCT negatif sağlıklı bireylerden oluşturuldu. Bulgular: Çalışmada; 34 aktif akciğer TB'li hastanın 22'sinde (duyarlılık %64.7) ve 29 kontrol grubu bireyin 3'ünde (özgüllük %89.7) 38 kDa'luk antijene karşı serolojik yanıt saptandı. Aktif akciğer TB grubunda; serolojik yanıt saptanan hastaların daha genç oldukları (39.7±17.2'ye karşı 53.3±19.1, p=0.04) ve kan örneklerinin tedavinin daha erken döneminde alınmış olduğu (3.9±5.2'ye karşı 8.6±7.7 gün, p=0.04) görüldü. Sonuç: Sonuç olarak; bu çalışmada elde edilen duyarlılık ve özgüllük oranları, TB tanısında altın standart olan kültürün yerini alabilecek bir serolojik testten beklenen duyarlılık (%80) ve özgüllük (%95) oranlarının altındadır.

Evaluation of the serological response to Mycobacterium tuberculosis antigen fractions by western blotting method in pulmonary tuberculosis

Aim: The aim of this study was to evaluate serological response to M.tuberculosis sonicate antigen fractions by Western blotting method in active pulmonary TB. Material and Methods: A total of 63 individuals, 34 active pulmonary TB and 29 control group, admitted to Ege University Medical Faculty, Department of Chest Diseases between September 2003 and September 2004 were included in the study. The control group was consisted of 12 inactive pulmonary TB, 14 TST positive healthy individuals and 3 TST negative healthy individuals. Results: In the study, serological response to 38 kDa antigen was detected in 22 of 34 (sensitivity 64.7%) active pulmonary TB and in 3 of 29 (specificity 89.7%) control group. The seropositive cases in active pulmonary TB group were found to be younger (39.7±17.2 versus 53.3±19.1 yrs, p=0.04) and the blood samples had been taken in the earlier period of the treatment (3.9±5.2 versus 8.6±7.7. days, p=0.04). Conclusion: In conclusion, the sensitivity and specifity rates in this study are lower than the expected sensitivity (80%) and specifity (95%) rates of a serologic test which can be used instead of the gold standart microbiologic culture for the diagnosis of TB.

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