Nazan UYSAL, Sevil GÖNENÇ, Ataç SÖNMEZ, İlkay AKSU, Ayça TOPÇU, Berkant Muammer KAYATEKİN, Osman AÇIKGÖZ

Adölesan sıçan beyninde antioksidan enzim aktiviteleri ve lipid peroksidasyon düzeyleri

Şizofreni, madde bağımlılığı, anksiyete, depresyon gibi pek çok hastalığın adölesans döneminde ortaya çıktığı bilinmektedir. Adölesans beyni nörotransmitter sistem, dopaminerjik sistem, yapı ve fonksiyonu bakımından adölesandan önceki ve sonraki dönemden farklıdır. Adölesans döneminde prefrontal korteks ve hipokampusta dopamin metabolizması artarken striatumda azalır. Dopamin metabolizmasındaki artış reaktif oksijen türlerinde artışa neden olarak oksidatif stresi tetikleyebilir. Bu çalışmanın amacı adölesan öncesi, adölesan ve erişkin sıçan prefrontal korteks, striatum ve hipokampusunda süperoksit dismutaz, glutatyon peroksidaz enzim aktiviteleriyle lipit peroksidasyon göstergesi olan tiyobarbitürik asitle reaksiyona giren maddelerin düzeylerinin araştırılmasıdır. Çalışmada 21 günlük (adölesan öncesi), 38 günlük (adölesan) ve 6 aylık (adölesan sonrası) sıçanlar kullanılmıştır. Bu çalışmada adölesan sıçanların prefrontal korteks ve hipokampusunda süperoksit dismutaz enzim aktivitesi ve tiyobarbitürik asitle reaksiyona giren maddelerin düzeyleri artarken glutatyon peroksidaz aktivitesinin azalmış olduğu görüldü. Adölesan striatumunda ise süperoksit dismutaz enzim aktivitelerinin ve tiyobarbitürik asitle reaksiyona giren maddelerin düzeylerinin düşük olduğu saptanmıştır. Bu sonuçlar, adölesan sıçan beyninde prefrontal korteks ve hipokampus bölgelerinin diğer dönemlere göre oksidan strese karşı daha savunmasız olduğunu düşündürmektedir.

Anahtar Kelimeler:

Modeller, hayvan, Süperoksit dismutaz, Lipid peroksidasyonu, Antioksidanlar, Beyin, Şizofreni, Hayvan deneyleri, Sıçan, Wistar, Ergen

Antioxidant enzyme activities and lipid peroxidation levels in andolescent rat brain

It is known that adolescent is a period in which symptoms moot disorders such as anxiety and depression or psychoses such as schizaprenia or behavioral abnormalities/such as drug abuse mostly emerge in the individual. Adolescent brain differ frompre and post adolescence in regard to neurotransmitter systems such as the dopaminergic system, as well as in organization and function. In adolescence, dopamine metabolism increases in prefrontal corteks and hippocampus, while it decreases in striatum. Increased metabolism of dopamine may evoke an oxidative stress derived from increased production of reactive oxygen species. The present study was designed to determine the superoxide dismutase and glutathione peroxidase enzyme activities and thiobarbituric acid reactive substances levels, an indicator of lipid peroxidation, in prefrontal cortex, hippocampus and striatum of 21 days old (pre-adolescents), 38 day old (adolescent) and 6 months old (post-adolescents) rats. We demonstrated that superoxide dismutase activity and thiobarbituric acid reactive substances levels increased in, but glutathione peroxidase activity decreased in prefrontal cortex and hippocampus of adolescent rats compared with other ages. In striatum, superoxide dismutase activity and thiobarbituric acid reactive substances levels decreased in adolescents compared with pre and post-adolescents. These results suggest that prefrontal cortex and hippocampus of adolescent rats may be more defenseless to oxidative stress, compared to other ages.

Keywords:

Models, Animal, Superoxide Dismutase, Lipid Peroxidation, Antioxidants, Brain, Schizophrenia, Animal Experimentation, Rats, Wistar, Adolescent,

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