In this study we investigated effects of Streptozotocin (STZ) administered at different doses on the total number of neurons in segment Thoracic 11 (T11) of the spinal cord of adult rats by using stereological methods. Twenty-eight adult male Wistar albino rats were used. The rats were divided into four groups; control group, saline group (0.5 ml of 0.9% NaCl i.p.), 45mg/kg of STZ (i.p.) group and 65 mg/kg of STZ (i.p) exposed group. At the seventh day, the rats were perfused with 10% neutral buffered formaldehyde. T11, which is one of the transverse spinal cord segments, was isolated and processed for routine histologic examination. Semithin sections (0.5µm thick) were obtained using a ultramicrotome according to systematic random sampling strategies. The sections were stained with Toluidine Blue with Borax. The motor neurons were counted unbiased stereological disector/Cavalieri combination method. The groups were compared statistically. In terms of motor neuron number, no significant difference among the groups was found (p>0.05). Our results indicated that exposure to different doses of Streptozotocin has no effect on the T11 segment of the spinal cord total motor neuron number of the adult rats.
___
Seino Y, Nanjo K, Tajima N, et al. Report of the committee on the classification and diagnostic criteria of diabetes mellitus. J Diabetes Investig 2010; 1: 212-228.
American Diabetes Association. Standards of medical care in diabetes. Diabetes Care 2014; 37: 14-80.
Said G. Focal and multifocal diabetic neuropathies. Arq Neuropsiquiatr 2007; 65: 1272- 1278.
Clemente CD. The back, vertebral column, and spinal cord. Anatomy A Regional Atlas of the Human Body, 6th Ed, Lippincott Williams & Wilkins; Philadelphia 2011; 369-408.
Graham ML, Janecek JL, Kittredge JA, et al. The streptozotocin-induced diabetic nude mouse model: differences between animals from different sources. Comp Med 2011; 61: 356-360.
Deeds MC, Anderson JM, Armstrong AS, et al. Single dose streptozotocin induced diabetes: considerations for study design in islet transplantation models. Lab Anim 2011; 45: 131- 140.
Toth C, Rong LL, Yang C, et al. Receptor for advanced glycation, products (RAGEs) and experimental diabetic neuropathy. Diabetes 2008; 57: 1002-1017.
Gundersen HJ. Stereology of arbitrary particles. A review of unibased number and size estimators and the presentations of some new ones, in memory of William R. Thompson. J Microsc 1986; 147: 229-263.
Pakkenberg B, Gundersen HJ. Total number of neurons and glial cells in human brain nuclei estimated by the disector and the fractionator. J Microsc 1988; 150: 1-20.
Howard CV, Reed MG. Unbiased stereology, three-dimensionel measurements in microscopy. BIOS Scientific Publishers Limited. Oxford 1998; 39-68.
Cakmak G, Soyguder Z, Ragbetli MC. A Morphological and stereological study on cervical segment of spinal cord of quails. Anat. Histol. Embryol 2017; 46: 258-266.
Gundersen HJ, Jensen EB. The efficiency of systematic sampling in stereology and its prediction. J Microsc 1987; 143: 3-45.
Boulton AJM, Vinik AI, Arezzo JC, et al. Diabetic neuropathies: A statement by the american diabetes association. Diabetes Care 2005; 2: 956- 962.
Schmeichel AM, Schmelzer JD, Low PA. Oxidative injury and apoptosis of dorsal root ganglion neurons in chronic experimental diabetic neuropathy. Diabetes 2003; 52: 165-171.
Toth C, Brussee V, Cheng C, et al. Diabetes mellitus and sensory neuron. J. Neuropathol Exp. Neurol 2004; 63: 561-573.
Ramji N, Zochodne DW, Toth C, et al. Does diabetes mellitus target motor neurons ? Neurobiol Dis 2007; 26: 301-311.
Souayah N, Potian JG, Garcia CC, et al. Motor unit number estimate as a predictor of motor dysfunction in an animal model of type 1 diabetes. Am J Physiol Endocrinol Metab 2009; 297: E602-E608.
Zochodne DW, Ramji N, Toth C. Neuronal targeting in diabetes mellitus: a story of sensory neurons and motor neurons. Neuroscientist 2008; 14: 311-318.