Börte KÖSE MUTLU, TÜRKER TÜRKEN, Selin OKATAN, Gamze DURMAZ, Melike ÜRPER BAYRAM, Serkan GÜÇLÜ, SÜLEYMAN ÖVEZ, İSMAİL KOYUNCU

Bizmut-BAL Şelatı Sentezinin Optimizasyonu ve Escherichia coli, Streptococcus pyogenes ve Aktif Çamur Üzerindeki İnhibisyon Etkisi

Teknoloji ve mühendisliğin gelişmesi ile beraber yüksek kalitede pek çok ürünün üretimi ve geniş kullanım alanlarında yeralması mümkün olmaktadır. Bu yüksek kalitedeki mikro- ve nano-teknolojik malzemeler bakteri kolonileri ve biyofilm tabakaları tarafından kolayca sarılmaktadırlar. Araştırmacılar, bu probleme çözüm bulabilmek adına pek çok farklı antibakteriyel kimyasal sentezlemişlerdir. Uzun bir süredir, hücredışı polimerik maddeler (EPS) ve çözünen mikrobiyal ürünler (SMP) sebebiyle porlarda ve yüzeylerde gerçekleşen biyofilm oluşumu mühendislik uygulamalarının bir problemi olarak gösterilmektedir. Mikroorganizmalar sentezlenen bizmut-BAL şelatı (Bis-BAL) na maruz kaldığında toplam polisakkaritleri ve proteinleri daha az salgılarlar. Bu çalışmada Escherichia coli ve Streptococcus pyogenes tarafından salgılanacak EPS ve SMP’nin engellenmesi öngörülmüştür. Bizmut metali ve lipofilik tiyolün çeşitli konsantrasyonlarının kombinasyonlar halinde denenmesi ile sentezlenen kimyasallar arasından en yüksek inhibisyon etkisini gönderenin belirlenmesi amaçlanmıştır. BisBAL’ın uygulanmasıda izlenebilecek stratejiler ve stabilitesi de çalışılmıştır. İlgili şelat membran biyoreaktörlerde kullanılacak olan membranların bünyesine ilave edilebilir ve böylelikle doğal su kaynaklarının kalitesinin korunarak su ihtiyacının karşılanmasında tercih edilen membran teknolojisinde biyotıkanmanın önüne geçilebilir.

Anahtar Kelimeler:

Antibakteriyel Ürün, Bizmut, Minimum İnhibisyon Konsantrasyonu, Karışık Kültür; Saf Bakteri Kültürü

Synthesis Optimization and Inhibition Effects of Bismuth-BAL Chelate on Escherichia coli, Streptococcus pyogenes, and Activated Sludge

With the development of technology and engineering, different highly qualified products can be manufactured and used with a wide range. This highly qualified micro- and nano-technological materials can be easily covered with bacteria colonies and biofilms. Researchers have tried to synthesize different anti-bacterial chemicals to solve this problem. The formation of biofilm on the surface and in the pores, which is caused by the extracellular polymeric substances (EPS) and soluble microbial products (SMP), has long been identified as a problem for engineering applications. The total polysaccharides and proteins secreted by microorganisms can be decreased when exposed to the synthesized bismuth-BAL chelate (BisBAL). Our proposal is to inhibit the EPS and SMP from Escherichia coli and Streptococcus pyogenes. The anti-bacterial properties of synthesized chemicals that have several combinations of bismuth metal with lipophilic thiol compound were examined to get the optimum inhibitor. The application strategies and stability of BisBAL were also studied. The chelate can be used as a compound of membranes used in membrane bioreactors, which will be an obligation of advanced wastewater treatment with the aims of the protection of natural water sources’ quality and supply the water demand, to prevent the biofouling.

Keywords:

Anti-bacterial Product Bismuth, Minimum Inhibition Concentration, Mixed Culture, Pure Bacteria Culture,

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