Seksual Siklus Süresince İneklerde Tuba Uterina’da ErbB1/HER1 ve ErbB2/HER2 Reseptörlerinin Dağılımı

Tuba uterina, memeli üremesinde çok önemli bir rol oynar ve oosit olgunlaşması, sperm kapasitasyonu, döllenme, gamet ve embriyoların taşınması gibi fonksiyonlar için en uygun ortamı sağlar. Epidermal Büyüme Faktörü Reseptörleri (erbB/HER) ailesinin tuba uterina’nın fonksiyonlarında etkili olduğu düşünülmektedir. Birçok araştırmacı ErbB1/HER1 ve ErbB2/HER2 reseptörlerinin seksual ya da menstrual siklus süresince tuba uterina’nın luminal epitel, bağdoku ve düz kas hücrelerinden değişen yoğunluklarda ekspresse olduğunu göstermişlerdir. Ekonomik kayıpların önüne geçebilmek için gebelik süresince etkin rol oynayan hormonal ve moleküler mekanizmaların en iyi şekilde anlaşılması gerekir. Sunulan çalışmada, erbB1/HER1 ve erbB2/HER2 reseptörlerinin ekspresyonu immunohistokimyasal olarak incelendi. Bu çalışmada 23 farklı Holstein ineğin ovaryum, uterus ve tuba uterina’sından doku örnekleri alındı. Tuba uterina’nın luminal epitel hücreleri, bağdoku ve düz kas hücreleri ile kan damarlarının endotel ve düz kas hücrelerinde değişen yoğunluklarda erbB1/HER1 ve ErbB2/HER2 reseptörleri için pozitif membran ve sitoplazmik boyanmaları elde edildi. Sonuç olarak, seksual siklus süresince erbB1/HER1 ve erbB2/HER2 reseptörlerinin inek tuba uterina’sından hücre tiplerine göre değişen yoğunluklarda lokalize olması, bu reseptörlerin inek genital sistem üzerinde önemli fizyolojik etkilere sahip olabileceğini akla getirdi.

Distribution of ErbB1 / HER1 and ErbB2 / HER2 Receptors in the Bovine Tuba Uterine During the Sexual Cycle

The tuba uterina plays a very important role in mammalian reproduction and provides the optimal environment for functions such as oocyte maturation, sperm capacitation, fertilization, and transport of gametes and embryos. Epidermal Growth Factor Receptors (erbB / HER) family is thought to be effective in the functions of the tubular uterina. Many researchers have shown that ErbB1 / HER1 and ErbB2 / HER2 receptors are expressed in varying densities from luminal epithelium, connective tissue and smooth muscle cells of the tubular uterine during the sexual or menstrual cycle. In order to prevent economic losses, hormonal and molecular mechanisms that play an active role during pregnancy should be understood in the best way. In the presented study, the expression of erbB1 / HER1 and erbB2 / HER2 receptors was examined immunohistochemically. In this study, tissue samples were taken from the ovary, uterus and tubular uterina of 23 different Holstein cows. Positive membrane and cytoplasmic staining were obtained for erbB1 / HER1 and ErbB2 / HER2 receptors at varying intensities in luminal epithelial cells, connective tissue and smooth muscle cells of the tubular uterina, and endothelial and smooth muscle cells of blood vessels. As a result, erbB1 / HER1 and erbB2 / HER2 receptors are localized at varying densities according to cell types in the cow tubular uterine during the sexual cycle, suggested that these receptors may have significant physiological effects in the bovine genital system.

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