Emel DIRAZ, Şengül KARAMAN, Hasan DURDU, Ashabil AYGAN, Sedat KÖSTEKCİ

Seasonal Variations and Antimicrobial Activity of Essential Oil Composition of Orthurus heterocarpus Boiss. Juz. from Turkey

Orthurus heterocarpus Boiss. Juz., Rosaceae familyasına ait olup, bitkinin toprak altı kısımları Kahramanmaraş İli’nin Göksun yöresinde halk arasında geleneksel olarak kullanılmaktadır. O. heterocarpus köklerinin uçucu yağlarının kompozisyonu GC-MS/FID ile belirlenmiştir. En önemli bileşen öjenol 95.80%-87.53% olarak tespit edilmiştir. Uçucu yağların antimikrobiyal etkisi dokuz bakteri Sarcina lutea, Enterobacter aerogenes, Bacillus subtilis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, MRSA metisilin dirençli Staphylococcus aureus , Pseudomonas aeruginosa, Klebsiella pneumonia ve bir mayaya Candida albicans karşı disk difüzyon yöntemi kullanılarak belirlenmiştir. Sonuçlar O. heterocarpus uçucu yağlarının geniş spektrumlu antimikrobiyal aktiviteye sahip olduğunu göstermiştir

Seasonal Variations and Antimicrobial Activity of Essential Oil Composition of Orthurus heterocarpus Boiss. Juz. from Turkey

The underground parts of Orthurus heterocarpus Boiss. Juz., belonging to Rosaceae family, are used traditionally by local people from Göksun province in Kahramanmaraş city and the seasonal variations of the essential oil of O. heterocarpus roots were analyzed by GC–MS/FID. Eugenol was found as a major component 95.80%-87.53% in the roots oil but no significant seasonal variations as statically . Antimicrobial activity of essential oil was also carried out using disk diffusion method against nine bacteria Sarcina lutea, Enterobacter aerogenes, Bacillus subtilis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, MRSA Methicillin-resistant Staphylococcus aureus , Pseudomonas aeruginosa, Klebsiella pneumonia and one yeast Candida albicans strain. The results revealed that the essential oil of O. heterocarpus shows a high broad spectrum antimicrobial activity.

Keywords:

Orthurus heterocarpus, Essential oil, Eugenol, Antimicrobial activity,

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