Hikmet ZEYTUN, Erol BASUGUY, İbrahim İBİLOĞLU, Serkan ARSLAN, İbrahim KAPLAN, M.Hanifi OKUR

Carvacrol’un Ratlarda Böbrek İskemi Reperüzyon Hasarı Üzerine Koruyucu Etkileri

Amaç: Ratlarda böbrek iskemi reperfüzyonu hasarı üzerine Carvacrol (CARV) 'ün koruyucu etkilerini araştırmaktır. Yöntemler: Ağırlıkları 200-250 gr arasında değişen, 30 adet erkek Wistar Albino ratlar rastgele grublara ayrıldı. 50 mg/kg ketamin ve 3mg/ kg ksilazin (periton içi) ile anestezi sağlandı. Renal arter trombozunu önlemek için 100 İU/kg heparin (periton içi) uygulamasından sonra orta hat kesi ile tüm ratlara sağ nefrektomi yapıldı. Grup I (Sham): Sağ nefrektomiyi takiben 6 saat sonra sol nefrektomi yapıldı. Grup II (I/R): Sağ nefrektomiyi takiben sol böbrek, 60 dakika iskemi ve 6 saat reperfüzyonda bırakıldı. Grup III(I/R+CARV): Grup II de uygulanan işleme ek olarak, iskemi ve reperfüzyondan hemen önce toplam 2 kez 35mg/kg periton içine Carvacrol verildi. Altı saatlik reperfüzyonu takiben ratların tamamı kardiak ponksiyonla yüksek miktarda kan alınarak sakrifiye edildi ve sol nefrektomi yapıldı. Alınan serum ve dokulardaki proiflamatuar sitokinler, oksidatif stres parametreleri çalışıldı ve histopatolojik inceleme yapılarak gruplar arasında karşılaştırma yapıldı. Bulgular: Böbrek fonksiyon göstergesi olan Serum BUN ve kreatinin düzeyleri, I/R grubunda Sham grubuna kıyasla yükselmiş ve iskeminin gerçekleştiğini göstermektedir(p

Protective Effects of Carvacrol on Renal Ischemia Reperfusion Damage in Rat

Objective: The aim of this study was to investigate the protective effect of Carvacrol (CARV) on renal ischemia reperfusion (I/R) injury in rats. Method: 30 male Wistar Albino rats weighing 200-250 gr were divided into random groups. Anesthesia was achieved with 50 mg / kg ketamine and 3 mg/kg xylazine (intraperitoneal). Right nephrectomy was performed to all rats by midline incision after 100 IU/kg heparin (intraperitoneal) application to prevent renal artery thrombosis. Subsequently, the left renal artery was clamped, created ischemia for 60 minutes. Group I (Sham): Left nephrectomy was performed six hours after right nephrectomy. Group II (I/R): Following right nephrectomy, left kidney was left for 60 minutes for ischemia and 6 hours for reperfusion. G roup I II ( I/R+CARV): In addition to t he p rocedure performed i n group I I, C arvacrol w as g iven 2 t imes 35 mg/ kg intraperitoneally just before ischemia and reperfusion. After six hours of reperfusion, all rats were sacrificed by cardiac puncture with a high volume of blood and left nephrectomy was performed. The parameters of oxidative stress and proinflammatory cytokines in the serum and tissues were analyzed and histopathological examination was performed to compare the groups. Results: Serum BUN and creatinine levels, which are indicators of kidney function, increased in Group II compared to Group I, which shows that ischemia occurred. (p

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