Mehmet TÜTÜNCÜ, Başar SEVİNDİK, Tolga İZGÜ, Özer YILMAZ, Gönül KAYNAK, Pembe EVCİ ÇÜRÜK, Yeşim MENDİ

Türkiye’de Doğal Olarak Yayılış gösteren Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Türü İçin Etkili Mikroçoğaltım Protokolü

Lamiaceae familyası, yüksek bioaktif bileşenleri sayesinde tıbbi ve aromatik özelliklerinden dolayı halk tıbbında kullanılan tek yıllık ve çok yıllık 250 cins içermektedir. Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum endemik olarak bilinen ve Türkiye’de yayılış gösteren Lamium cinsinin bir üyesidir. Lamium türleri eki çağlardan beri tedavi amaçlı kullanılmaktadır. Doku kültürü, endemik türlerin korunması ve sürdürülebilirliği açısından klonal çoğaltım ve sınırlı alanda kitlesel üretim gibi önemli avantajlar sağlar. Mikroçoğaltım kısa sürede klonal çoğaltım için çok önemli bir alternatiftir. Bu çalışmada bir ya da iki yaprak içeren nod eksplantları çoğaltım amacıyla farklı seviyelerde BA ve GA3 (0, 0.5, 1, 1.5, 2, 2.5 mg/L) içeren MS besi yerine yerleştirilmiştir. Eksplantlar %82.5 ve %80 oranında sırasıyla 2 mg/L BA +2 mg/L GA3, 2 mg/L BA +1.5 mg/L GA3 içeren MS besi yerinde sürgüne rejenere olmuşlardır. Köklenme denemeleri 0, 0.5, 1, 1.5, 2 mg/L Indol-3- Bütirik Asit (IBA) içeren MS besi yerinde gerçekleştirilmiştir. Maksimum kök oluşumu 0.5 mg/L IBA içeren MS besi yerinde %45 olarak gözlemlenmiştir. Bitkiler dışa aktarılmış ve bitkilerin aklimatizasyondan sonra %80 oranında hayatta kaldığı belirlenmiştir.

Anahtar Kelimeler:

Lamiacaeae, Lamium sp, Endemik, Mikroçoğaltım, Köklenme, Genetik Kaynak

Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey

Lamiaceae family includes annual and perennial 250 genus which are commonly used for their medicinal aromatic properties due to its high biologically active compounds. Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum is a member of Lamium genus spread over in Turkey and known as endemic. Lamium species are used for cure since ancient times. Tissue culture provides important advantages for the conservation and sustainability of endemic species such as clonal and mass production in a limited area. Micropropogation is one of the most significant alternative for rapid clonal propagation in a short time. In this study, nodes include one or two leaves were placed on MS medium including different levels of BA and GA3 (0, 0.5, 1, 1.5, 2, 2.5 mg/L) for propagation. The explants regenerated to the shoots with the response of 82.5% and 80% in MS medium including 2 mg/L BA +2 mg/L GA3, 2 mg/L BA +1.5 mg/L GA3 respectively. Rooting experiment was carried on MS medium supplied with 0, 0.5, 1, 1.5, 2 mg/L Indole-3- Butyric Acid (IBA) for root induction. Maximum root formation was observed as 45% on MS medium including 0.5 mgL-1 IBA. Plantlets were acclimatized and 80% of the plants are survived after acclimatization.

Keywords:

Lamiaceae, Lamium sp., endemic, micropropogation, rooting, genetic resources,

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