Türkiye’de Doğal Olarak Yayılış gösteren Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Türü İçin Etkili Mikroçoğaltım Protokolü

Lamiaceae familyası, yüksek bioaktif bileşenleri sayesinde tıbbi ve aromatik özelliklerinden dolayı halk tıbbında kullanılan tek yıllık ve çok yıllık 250 cins içermektedir. Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum endemik olarak bilinen ve Türkiye’de yayılış gösteren Lamium cinsinin bir üyesidir. Lamium türleri eki çağlardan beri tedavi amaçlı kullanılmaktadır. Doku kültürü, endemik türlerin korunması ve sürdürülebilirliği açısından klonal çoğaltım ve sınırlı alanda kitlesel üretim gibi önemli avantajlar sağlar. Mikroçoğaltım kısa sürede klonal çoğaltım için çok önemli bir alternatiftir. Bu çalışmada bir ya da iki yaprak içeren nod eksplantları çoğaltım amacıyla farklı seviyelerde BA ve GA3 (0, 0.5, 1, 1.5, 2, 2.5 mg/L) içeren MS besi yerine yerleştirilmiştir. Eksplantlar %82.5 ve %80 oranında sırasıyla 2 mg/L BA +2 mg/L GA3, 2 mg/L BA +1.5 mg/L GA3 içeren MS besi yerinde sürgüne rejenere olmuşlardır. Köklenme denemeleri 0, 0.5, 1, 1.5, 2 mg/L Indol-3- Bütirik Asit (IBA) içeren MS besi yerinde gerçekleştirilmiştir. Maksimum kök oluşumu 0.5 mg/L IBA içeren MS besi yerinde %45 olarak gözlemlenmiştir. Bitkiler dışa aktarılmış ve bitkilerin aklimatizasyondan sonra %80 oranında hayatta kaldığı belirlenmiştir.

Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey

Lamiaceae family includes annual and perennial 250 genus which are commonly used for their medicinal aromatic properties due to its high biologically active compounds. Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum is a member of Lamium genus spread over in Turkey and known as endemic. Lamium species are used for cure since ancient times. Tissue culture provides important advantages for the conservation and sustainability of endemic species such as clonal and mass production in a limited area. Micropropogation is one of the most significant alternative for rapid clonal propagation in a short time. In this study, nodes include one or two leaves were placed on MS medium including different levels of BA and GA3 (0, 0.5, 1, 1.5, 2, 2.5 mg/L) for propagation. The explants regenerated to the shoots with the response of 82.5% and 80% in MS medium including 2 mg/L BA +2 mg/L GA3, 2 mg/L BA +1.5 mg/L GA3 respectively. Rooting experiment was carried on MS medium supplied with 0, 0.5, 1, 1.5, 2 mg/L Indole-3- Butyric Acid (IBA) for root induction. Maximum root formation was observed as 45% on MS medium including 0.5 mgL-1 IBA. Plantlets were acclimatized and 80% of the plants are survived after acclimatization.

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Kaynak Göster

Bibtex @araştırma makalesi { cutarim829769, journal = {Çukurova Tarım ve Gıda Bilimleri Dergisi}, issn = {2636-7874}, eissn = {2630-6034}, address = {Çukurova Tarım Gıda Bil. Der. Çukurova Üniversitesi Ziraat Fakültesi Yayın Ünitesi Balcalı-Sarıçam 01330 Adana}, publisher = {Çukurova Üniversitesi}, year = {2020}, volume = {35}, pages = {89 - 98}, doi = {10.36846/CJAFS.2020.22}, title = {Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey}, key = {cite}, author = {Tütüncü, Mehmet and Sevindik, Başar and İzgü, Tolga and Yılmaz, Özer and Kaynak, Gönül and Evci Çürük, Pembe and Mendi, Yeşim} }
APA Tütüncü, M , Sevindik, B , İzgü, T , Yılmaz, Ö , Kaynak, G , Evci Çürük, P , Mendi, Y . (2020). Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey . Çukurova Tarım ve Gıda Bilimleri Dergisi , 35 (2) , 89-98 . DOI: 10.36846/CJAFS.2020.22
MLA Tütüncü, M , Sevindik, B , İzgü, T , Yılmaz, Ö , Kaynak, G , Evci Çürük, P , Mendi, Y . "Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey" . Çukurova Tarım ve Gıda Bilimleri Dergisi 35 (2020 ): 89-98 <https://dergipark.org.tr/tr/pub/cutarim/issue/58979/829769>
Chicago Tütüncü, M , Sevindik, B , İzgü, T , Yılmaz, Ö , Kaynak, G , Evci Çürük, P , Mendi, Y . "Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey". Çukurova Tarım ve Gıda Bilimleri Dergisi 35 (2020 ): 89-98
RIS TY - JOUR T1 - Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey AU - Mehmet Tütüncü , Başar Sevindik , Tolga İzgü , Özer Yılmaz , Gönül Kaynak , Pembe Evci Çürük , Yeşim Mendi Y1 - 2020 PY - 2020 N1 - doi: 10.36846/CJAFS.2020.22 DO - 10.36846/CJAFS.2020.22 T2 - Çukurova Tarım ve Gıda Bilimleri Dergisi JF - Journal JO - JOR SP - 89 EP - 98 VL - 35 IS - 2 SN - 2636-7874-2630-6034 M3 - doi: 10.36846/CJAFS.2020.22 UR - https://doi.org/10.36846/CJAFS.2020.22 Y2 - 2020 ER -
EndNote %0 Çukurova Tarım ve Gıda Bilimleri Dergisi Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey %A Mehmet Tütüncü , Başar Sevindik , Tolga İzgü , Özer Yılmaz , Gönül Kaynak , Pembe Evci Çürük , Yeşim Mendi %T Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey %D 2020 %J Çukurova Tarım ve Gıda Bilimleri Dergisi %P 2636-7874-2630-6034 %V 35 %N 2 %R doi: 10.36846/CJAFS.2020.22 %U 10.36846/CJAFS.2020.22
ISNAD Tütüncü, Mehmet , Sevindik, Başar , İzgü, Tolga , Yılmaz, Özer , Kaynak, Gönül , Evci Çürük, Pembe , Mendi, Yeşim . "Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey". Çukurova Tarım ve Gıda Bilimleri Dergisi 35 / 2 (Aralık 2020): 89-98 . https://doi.org/10.36846/CJAFS.2020.22
AMA Tütüncü M , Sevindik B , İzgü T , Yılmaz Ö , Kaynak G , Evci Çürük P , Mendi Y . Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey. Çukurova Tarım Gıda Bil. Der.. 2020; 35(2): 89-98.
Vancouver Tütüncü M , Sevindik B , İzgü T , Yılmaz Ö , Kaynak G , Evci Çürük P , Mendi Y . Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey. Çukurova Tarım ve Gıda Bilimleri Dergisi. 2020; 35(2): 89-98.
IEEE M. Tütüncü , B. Sevindik , T. İzgü , Ö. Yılmaz , G. Kaynak , P. Evci Çürük ve Y. Mendi , "Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey", Çukurova Tarım ve Gıda Bilimleri Dergisi, c. 35, sayı. 2, ss. 89-98, Ara. 2021, doi:10.36846/CJAFS.2020.22