Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri

Amaç: Kemoterapi kaynaklı prematür over yetmezliği (POY) tedavisi için önerilebilecek yağ doku mezenkimal kök hücreleri (YDMKH) ve bu hücrelerden elde edilmiş koşullu besiyerinin (KB) oksidatif stres ve apoptoz üzerine etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Çalışmada Wistar albino cinsi erişkin dişi sıçanlar 4 gruba ayrıldı. Kontrol, POY, POY+YDMKH, POY+KB. Ovaryumda histopatolojik değerlendirmeler için H&E boyamaları yapıldı ve tüm ovaryum folilkülleri sayıldı. Oksidatif stres ve apoptoz belirteci olarak iNOS ve Caspase3 ekspresyonları immunohistokimyasal olarak gösterildi. Bulgular: Analizler sonucu POY grubu ovaryumlarında foliküller hücre dejenerasyonları, vakuolizasyon, gibi dejenerasyonlar gözlenirken, YDMKH ve KB gruplarında bu dejenerasyonlarda anlamlı azalmalar görüldü. POY grubunda artmış olan iNOS ve Caspase3 ekspresyonları YDMKH ve KB gruplarının her ikisinde de istatistiksel olarak anlamlı şekilde azaldı. Sonuç: Rejeneratif tıp alanında her geçen gün kullanımı artan mezenkimal kök hücrelerin ve hücresiz koşullu besiyerinin prematür over yetmezliğinde hastalığın temel mekanizmalarından olan apoptoz ve oksidatif stresin azaltılması yoluyla, POY için alternatif bir tedavi stratejisi olabileceği gösterildi.

Effects of adipose tissue derived mesenchymal stem cells and conditioned medium on experimental premature ovarian insufficiency model

Purpose: The aim of this study was to investigate the effects of adipose tissue derived mesenchymal stem cells (ADMSC) and conditioned medium (CM), which can be recommended for the treatment of chemotherapy-induced premature ovarian insufficiency (POI), on oxidative stress and apoptosis. Materials and Methods: In the study, adult female Wistar albino rats were divided into 4 groups; Control, POI, POI+ADMSC, POI+CM. H&E staining was performed for histopathological evaluations in the ovary and all ovarian follicles were counted. iNOS and Caspase3 expressions were shown immunohistochemically as markers of oxidative stress and apoptosis. Results: Follicular cell degenerations and vacuolization were observed in the ovaries of the POI group, while significant decreases were observed in these degenerations in the ADMSC and CM groups. Increased iNOS and Caspase3 expressions in the POI group were statistically significant decreased in both the ADMSC and CM groups. Conclusion: Mesenchymal stem cells and cell-free conditioned medium, which are increasingly used in the field of regenerative medicine, can be an alternative treatment strategy for POI by reducing apoptosis and oxidative stress, which are the main mechanisms of the disease.

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Cukurova Medical Journal-Cover
  • ISSN: 2602-3032
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 1976
  • Yayıncı: Çukurova Üniversitesi Tıp Fakültesi
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