Mustafa Ertan AY, Özlem İZCİ AY, Kenan ÇEVİK, Gurbet DOĞRU, Fatma SÖYLEMEZ, Filiz Evşen ÇAYAN, Mehmet Emin ERDAL

Tekrarlayan gebelik kayıplarında FAS ve FASLG polimorfizmlerinin TaqMan SNP genotiplendirme yöntemi ile belirlenmesi

Amaç: Bu çalışmada apoptotik uyarıcı Fas hücre yüzey ölüm reseptörü (FAS) ve FAS ligandı genleri (FASLG) polimorfizmlerinin tekrarlayan gebelik kaybı (TGK) etiyolojisindeki yeri araştırıldı.Gereç ve Yöntem: Bu vaka-kontrol çalışmasında, TGK tanısı almış 70 kadın ve kontrol grubu olarak 70 kadından genomik DNA izole edildi. Her iki gruptaki FAS -670 A>G, FAS -1377 G>A ile FASLG -124 A>G polimorfizmlerinin genomik dağılımı ve allel frekansları real-time PCR yöntemi ile belirlenerek karşılaştırıldı.Bulgular: Çalışmamızda, FASLG -124 A>G polimorfizmi için TGK ve kontrol gruplarının genotip frekans farkının istatistiksel olarak anlamlı olduğu saptandı. Ancak FAS −670 A>G ve FAS-1377 G>A genotipleri ile TGK arasında istatistiksel düzeyde anlamlı bir ilişki saptanmadı. FASLG −124 A>G polimorfizmi için A allelini taşıyanlarda hastalığın görülme olasılığı A allelini taşımayanlara göre 3,94 kat daha yüksek bulundu.Sonuç: Bulgularımız, apoptozla ilişkili FASLG -124 A>G polimorfizminin TGK oluşumuna katkısı olabileceğini düşündürmektedir.

Anahtar Kelimeler:

Apoptoz, Tekrarlayan gebelik kaybı, FAS, FASLG

Detection of FAS and FASLG polymorphisms with TaqMan SNP genotyping assays in recurrent miscarriage

Purpose: The aim of this study was to investigate the role of Fas cell surface death receptor (FAS) and FAS ligand genes (FASLG) polymorphisms in the etiology of recurrent miscarriage. Materials and Methods: In a case–control study, genomic DNA was obtained from 70 patients and 70 healthy controls. Detected with real time PCR, genotype distributions and allelic frequencies of polymorphisms of FAS -670 A>G, FAS-1377 G>A, FASLG -124 A>G genes were compared between the groups.Results: In this study, FASLG -124 A>G polymorphisms were associated with an increased risk of recurrent miscarriage whereas FAS −670 A>G and -1377 G>A genotypes conferred no risk. For the FASLG −124 A>G polymorphisms, the likelihood of disease in patients with A allele was 3.94 times higher than those without A allele. Conclusion: Our findings suggest that the -124 A>G polymorphism in the FASLG gene related with apoptosis may contribute to susceptibility to recurrent miscarriage in the Turkish women.

Keywords:

Apoptosis Recurrent miscarriage, FAS, FASLG, Polymorphisms,

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