Pulslu manyetik alanın λ-carrageenan’ın indüklediği akut pençe ödeminde antioksidan sistem üzerine etkisi

Amaç: λ-Carrageenan ile indüklenen (λ-Carr) pençe ödemi, inflamasyonun akut evresinin belirlenmesinde yaygın olarak kullanılan bir testtir. Bu çalışmanın amacı, λ-Carr'ın indüklediği sıçan pençe ödeminde pulslu manyetik alanın (PMF) antioksidan enzim düzeylerine etkinlğinin olup olmadığını araştırmaktı. Gereç ve Yöntem: Sıçanlar, λ-Carr injeksiyonundan 3 saat sonra 4 gün boyunca her gün günde 1 saat 1.5 mT şiddetinde ardışık 1, 10, 20, 40 Hz frekansları veren Helmholtz bobinlerinden oluşan pulslu manyetik alan etkisine maruz bırakıldılar. Dördüncü gün sıçanlar dekapite edildi ve pençelerindeki ödem değerlendirildi; Önce ayak bileği kesilerek pençe çıkarıldı, sağ ve sol pençe kütleleri (g) ve pençe kalınlıkları (mm) ölçüldü. Sonra pençelerde bulunan dokular lipit peroksidasyon (katalaz (CAT)) ve antioksidan enzim (malondialdehid (MDA)) aktivitelerini belirlemek için ayrıldı. Bulgular: Çalışmamızda sıçanların sol pençeleri , λ-Carr ile indüklenen sağ pençeler ile karşılaştırıldığında λ-Carr ile indüklenen sağ pençelerde kütlelerinin, pençe kalınlıklarının, CAT ve MDA enzim seviyelerinin arttığı görüldü. PMA uygulamasından sonra sağ pençelerin ağırlığı ve kalınlıklarında anlamlı değişiklik olmazken CAT enzimi azaldı, MDA enzim seviyesi ise arttı. Sonuç: PMF sıçan pençesinde ödemi arttırdı, antioksidan enzim seviyelerini de negatif olarak etkiledi.

Anahtar Kelimeler:

λ-Carrageenan, akut inflamasyon modeli, pulslu manyetik alan, antioksidanlar

Pulsed magnetic field action on antioxidant system in λ-carrageenan induced acute paw edema

Purpose: λ-Carrageenan-induced (λ-Carr) paw edema is a commonly used test for determining the acute phase of inflammation. In the present study, our aim was to investigate whether efficacy of pulsed magnetic field (PMF) on antioxidant enzymes levels is λ-Carr-induced rat paw edema.Material and Methods: The rats were whole-body exposed to PMF (1.5 mT intensity and 1, 10, 20, 40 Hz consecutive frequencies between Helmholtz coils) at the same hour in an hour in a day throughout 4 days at 3 hour later from injection of λ-Carr. Paw edema was determined at the end of fourth day by killing the rats, removed the paws at the ankle joint and paw mass (g) and paw thickness (mm) were was determined the right and left paw of rats. Then, the paw tissues were collected for the measurement of lipid peroxidation (Malondialdehyde; MDA) and antioxidant enzymatic activity levels (Catalase; CAT).Results: Carrageenan-induced right paw masses and paw thicknesses increased in compare to left paw of rats. While CAT levels were significantly decreased, MDA levels were increased in the carrageenan-induced rat right hind paw compare to control rat left paw.After exposed PMF to λ-Carr-induced right paw, while the CAT enzyme level decreased, the MDA enzyme level increased and there was no significant change in the weight and thickness of the right paw compare to unexposed groups. Conclusion: PMF increased edema in the rat paw and negatively affected antioxidant enzyme levels.

Keywords:

Pulsed magnetic field, carreganan, acute inflammation model, antioxidants,

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