Emin KAYMAK, Murat ÜNSAL, Ali AKIN, Emel ÖZTÜRK, Tayfun CEYLAN, Nurhan KULOĞLU, Derya KARABULUT, Birkan YAKAN

Melatoninin sıçanlarda cisplatin kaynaklı karaciğer hasarı üzerindeki koruyucu etkisi

Amaç: Cisplatin ile oluşturulan deneysel hepatatoksisite üzerine melatoninin koruyucu etkisini değerlendirmeyi amaçladık. Gereç ve Yöntem: Toplam 40 adet Wistar Albino türü rat kullanıldı. Kontrol grubuna (n=10) günlük 0.1 mg / kg izotonik solüsyon (intraperitoneal) i.p. uygulandı. Cisplatin grubuna (n = 10) 5. gün tek doz i.p. Sisplatin (7 mg / kg) uygulandı. Melatonin grubuna (n = 10) günlük i.p. Melatonin (10 mg / kg) uygulandı. Cisplatin + Melatonin grubuna (n = 10) günlük i.p. Melatonin (10 mg / kg) + 5. günde tek doz i.p. Cisplatin (7mg / kg) uygulandı. Deneyin 8. günü ratlardan karaciğer dokuları histolojik ve immünohistokimyasal olarak Tümör nekrozis faktör-α (TNF-α) inceleme için alındı. Kanlardan elde edilen serumlardan da karaciğer fonksiyonu için Alanin aminotransferaz (ALT), aspartat aminotransferaz (AST), Laktat dehidrogenaz (LDH) ve albümin seviyeleri için analizler yapıldı. Bulgular: Cisplatin uygulamasıyla birlikte karaciğer dokusu histopatolojik skorunun ve TNF-α immünoreaktivitesinin kontrol grubuna göre anlamlı olarak artış gösterdiği görülmüştür. Melatonin ile tedavi edilen grupta ise histopatolojik skorunun ve TNF-α immünoreaktivitesinin azaldığını ayrıca karaciğer fonksiyonu enzimleri olan ALT, AST ve LDH’ın cisplatin grubuna göre anlamlı şekilde azaldığını bulduk. Albümin düzeyi ise melatonin ile tedavi edilen grupta önemli bir iyileşme gösterdi. Sonuç: Melatoninin, sisplatinin neden olduğu hepatotoksisitede inflamasyonu azaltarak ve karaciğer enzimlerindeki artışı önleyerek koruyucu rol oynayabilir.

Anahtar Kelimeler:

Cisplatin, melatonin, inflamasyon

Protective effect of melatonin on cisplatin-induced liver injury in rats

Purpose: We aimed to evaluate the protective effect of melatonin (Mel) on experimental hepatatoxicity induced by cisplatin (Cis). Materials and Methods: A total of 40 Wistar Albino rats were used. Control group (n = 10) daily 0.1 mg / kg isotonic solution (intraperitoneal) i.p. was administered. Cis group (n = 10) was given a single dose of i.p. Cis (7 mg/kg) was administered. Melatonin (Mel) group (n = 10) daily i.p. Mel (10 mg / kg) was administered. Cis + Mel group (n = 10) daily i.p. Mel (10 mg / kg) + single dose i.p. Cis (7mg / kg) was administered. On the 8th day of the experiment, liver tissues of rats were collected histologically and immunohistochemically for tumor necrosis factor-α (TNF-α) analysis. Analyzes were performed for the levels of Alanine aminotransferase (ALT), aspartate aminotransferase (AST), Lactate dehydrogenase (LDH), and albumin for liver function from the serum obtained from the blood. Results: It was observed that liver tissue histopathological score and TNF-α immunoreactivity increased significantly with cisplatin administration compared to the control group. We found that the histopathological score and TNF-α immunoreactivity were decreased in the group treated with melatonin, and the liver function enzymes ALT, AST, and LDH were significantly decreased compared to the cisplatin group. Albumin level, on the other hand, showed a significant improvement in the group treated with melatonin. Conclusion: Melatonin may play a protective role in hepatotoxicity caused by cisplatin by reducing inflammation and preventing the increase in liver enzymes

Keywords:

Cisplatin, inflammation, melatonin,

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