Bülent KARAKAYA, Tuncay KULOĞLU, Erhan ÖNALAN, Nalan KAYA TEKTEMUR, Emir DÖNDER

Karbon tetraklorür ile oluşturulan deneysel karaciğer hasarında benfotiamin’in karaciğer dokusu üzerine koruyucu etkilerinin incelenmesi

Amaç: Bu çalışmada karbon tetraklorür (CCl4) ile oluşturulan deneysel karaciğer hasarında benfotiaminin karaciğer dokusu üzerine koruyucu etkilerinin incelenmesi amaçlanmıştır.Gereç ve Yöntem: Çalışmada, 30 adet Wistar albino cinsi erkek sıçanlar kullanıldı. Deney hayvanları 5 eşit gruba ayrıldı. Kontrol grubuna 14 günlük deney süresince herhangi bir uygulama yapılmadı. CCl4 grubuna ise 1. ve 8. günlerde 1ml/kg CCl4: zeytinyağı (1:2) intraperitoneal (i.p) verildi. CCl4 +benfotiamin grubuna 1. ve 8. günlerde iki defa 1 ml/kg CCl4: zeytinyağı (1:2) karışımı ve 70 mg/kg/gün oral benfotiamin verildi. Benfotiamin grubuna 70 mg/kg/gün oral benfotiamin verildi. Zeytinyağı grubuna 1. ve 8. günlerde 2 ml/kg zeytinyağı i.p. olarak verildi. Deney sonunda sıçanlar anestezi altında dekapite edildi. Karaciğer dokuları çıkarılıp incelemeler için parafin bloklar hazırlandı. Ayrıca malondialdehid (MDA) çalışması için dokular – 80 0 C de saklandı.Bulgular: Kontrol, benfotiamin ve zeytinyağı grupları arasında bakılan parametrelerde anlamlı bir farklılık yoktu. Kontrol grubu ile karşılaştırıldığında CCl4 grubunda, MDA, apoptozis ve bax immünreaktivitesinde anlamlı artış izlendi. CCl4 grubu ile karşılaştırıldığında CCl4 + benfotiamin grubunda ise MDA, apoptozis ve bax immünreaktivitesinde anlamlı azalma görüldü. Sonuç: CCl4’ün MDA, apoptozis ve bax immünreaktivitesini arttırdığı, tedavi olarak verilen benfotiaminin bu parametreleri azalttığı görüldü.

Anahtar Kelimeler:

Sıçan, karbon tetraklorür, benfotiamin

Investigation of benfotiamine’s protective effects on liver tissue in experimental carbon tetrachloride induced liver injury

Purpose: In this study, we aimed to investigate the protective effects of benfotiamine on experimental liver injury caused by carbon tetrachloride (CCl4).Materials and Methods: In this study, 30 male Wistar albino rats were used. Rats were equally divided into 5 groups. No application was made to control group. The CCl4 group was injected i.p with1ml/kg CCl4:olive oil (1:2) mixture on the 1st and 8th days, and the CCl4+benfotiamine group was treated i.p with 1 ml/kg CCl4: olive oil (1:2) mixture twice on the 1st and 8th days and orally with 70 mg/kg/day benfotiamine. To the benfotiamine group, 70 mg/kg/day benfotiamine was given orally for 14 days. To the olive oil group, 2 ml/kg olive oil was given i.p. on 1st and 8th days. Finally, rats were decapitated. Liver tissues were removed and paraffin blocks were prepared. Tissues were stored at –80 oC for malonaldeyhde (MDA) assay.Results: There were no significant differences between the control, benfotiamine and olive oil groups. Compared with the control group, there was a significant increase in MDA, apoptosis and bax immunoreactivity in CCl4 group. Compared with the CCl4 group, there was a significant decrease in MDA, apoptosis and bax immunoreactivity in the CCl4+benfotiamine group.Conclusion: CCl4 increases MDA, apoptosis and bax immunoreactivity, and benfotiamine, given as treatment, reduces these parameters.

Keywords:

Rat, carbon tetrachloride, benfotiamine,

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