Suat ÇAKINA, Selma YÜCEL, Cemre Çağan POLAT, Şamil ÖZTÜRK

Huzursuz bacak sendromu olan multipl sklerozlu hastalarda alfa-sinüklein düzeyleri

Amaç: Huzursuz Bacak Sendromu, multipl skleroz hastalığında yaygın olarak görülmektedir, ayrıca hastaların genel sağlığı ve yaşam kalitesi üzerinde olumsuz bir etkisi vardır. Alfa-sinüklein, Dopamin 2 reseptörünün sinyal yolağında önemli bir rol oynamaktadır. Çalışmalar, dopamin 2 reseptörü ve huzursuz bacak sendromu hastalığı arasında ilişki olduğunu göstermiştir. Bu çalışma huzursuz bacak sendromlu multipl skleroz hastalarında alfa-sinükleinin düzeyini belirlemek amacıyla yapılmıştır.

Anahtar Kelimeler:

Huzursuz bacak sendromu, multipl skleroz, alfa-sinüklein, RT-PCR, gen ekspresyonu

Alpha-synuclein levels in multiple sclerosis patients with restless leg syndrome

Purpose: The restless legs syndrome is more frequent and critical in Multiple Sclerosis patients, and it harms their general well-being and life quality. Alpha-synuclein is a synuclein protein that can have an impact on the pathway of signaling, affecting the Dopamin 2 receptor and its receptor trafficking. Studies have shown that the decrease in dopamine 2 receptor and Restless Legs Syndrome disease are correlated. This study is aimed to ascertain the alpha-synuclein level in multiple sclerosis patients with restless legs syndrome.Materials and Methods: We took blood samples from 40 multiple sclerosis patients and 20 healthy individuals. Half of the patient group had Multiple Sclerosis with restless leg syndrome. In the study groups, the alpha-synuclein level was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-dependent immunosorbent assay (ELISA).Results: Alpha-synuclein gene expression level was found or be significantly lower in restless leg syndrome patients with multiple sclerosis than the Alpha-synuclein gene expression level in the control group. Conclusion: Alpha-synuclein may have an impact on the pathogenesis of the restless leg syndrome of multiple sclerosis disease. Further investigations are required to determine the impact of alpha-synuclein in the pathogenesis of restless leg syndrome in multiple sclerosis disease.

Keywords:

Restless leg syndrome, multiple sclerosis, alpha-synuclein, RT-PCR,

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