Derya KARABULUT, Emel ÖZTÜRK, Ali Tuğrul Tuğrul AKİN, Ayça LEKESİZCAN, Hacı Murat Murat ÜNSAL, Tuğçe Merve ÖZYAZGAN, Meryem SAYAN

CCl4-nefrotoksisitesinde L-karnitin’in etkileri: Glomeruler nefrin ve HIF-1alfa ekspresyonlarının immunohistokimyasal değerlendirilmesi

Amaç: Bu çalışmada glomerül yapısındaki nefrin ve hipoksi indüklenebilir faktör-1alfa ekspresyonları aracılığıyla karbontetraklorid (CCl4) toksisitesi sonrası L-karnitin’in etkilerinin gösterilmesini amaçladık. Gereç ve Yöntem: 40 adet Sprague dawley erkek sıçan 5 gruba (n=8) ayrıldı. Grup I: Kontrol grubu; 0.2 ml zeytinyağı intraperitoneal (ip) haftada 2 kez, Grup II: L-karnitin grubu; 200 mg/kg L-karnitin (ip) haftada 2 kez, Grup III: CCl4 group; 0.2 ml CCl4 (ip) haftada 2 kez 6 hafta boyunca, Grup IV: L-karnitin + CCl4 grubu, haftada 2 kez CCl4 uygulamasından önce 200 mg/kg ip L-karnitin, Grup V: CCl4 + L-karnitin grubu, haftada 2 kez CCl4 uygulamasından 1 saat sonra 200 mg/kg ip L-karnitin. Böbrek doku kesitlerine nefrin ve HIF-1α ekspresyonunu göstermek için immunohistokimya boyama uygulandı. Proteinlerin ekspresyon yoğunlukları ImageJ programında ölçüldü.Bulgular: Nefrin ekspresyonu diğer gruplar ile kıyaslandığında Grup III’de anlamlı olarak arttı. HIF-1α ekspresyonu yalnızca Grup I ve Grup III arasında anlamlı şekilde arttı. Proteinlerin ekspresyon yoğunlukları L-karnitin-tedavili gruplarda kontrol grubuna benzerdi.Sonuç: L-karnitin böbrek glomerulusunda CCl4 toksisitesine karşı hem koruyucu hem tedavi edici etkilere sahiptir.

Anahtar Kelimeler:

Karbontetraklorid, L-karinitin, böbrek, nefrin

L-carnitine effects in CCl4-nephrotoxicity: Immunohistochemical evaluation of glomerular nephrin and HIF-1alpha expressions

Purpose: In this study, we aimed to demonstrate the effects of L-carnitine after carbontetrachloride (CCl4) toxicity through nephrin and Hypoxia inducible factor-1 alpha (HIF-1α) expressions in the glomerular structure.Materials and Methods: Forty male Sprague dawley rats were divided into 5 groups with animals in each group. Group I: Control group; 0.2 ml olive oil intraperitoneal (ip) twice weekly, Group II: L-carnitine group; 200 mg/kg L-carnitine (ip) twice a week, Group III: CCl4 group; 0.2 ml CCl4 (ip) twice a week for 6 weeks, Group IV: L-carnitine + CCl4 group, 200 mg/kg ip L-carnitine 24 hours before CCl4 twice a week, Group V: CCl4 + L-carnitine group; 200 mg/kg L-carnitine half an hour after CCl4 twice a week. Immunohistochemical staining was performed on kidney tissue sections to show nephrin and HIF-1α expression. Expression densities of the proteins were measured by ImageJ program.Results: Nephrin expression was significantly increased in Group III compared to other groups. There was a significant increase in HIF-1α expression only between Group I and Group III. Expression densities of proteins in L-carnitine-treated groups were similar to control.Conclusion: L-carnitine has both protective and therapeutic effects against CCl4 toxicity in renal glomeruli.

Keywords:

Carbontetrachloride, L-carnitine, kidney, nephrin,

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