Tıbbi Önemi Olan Hyoscyamus niger L.: Fenolik Madde İçeriği ve in vitro Antiproliferatif Aktiviteleri

Tıbbi önemi olan bitkiler, zengin bileşenleri nedeniyle kanser de dahil çeşitli hastalıkların tedavisinde en önemli ilaç kaynaklarından birini temsil etmektedirler. Bu çalışmanın amacı, Hyoscyamus niger bitkisinin toprak üstü kısımlarının metanol (HNM) ve su (HNS) ekstraktlarının 17 farklı fenolik madde içeriğini araştırmak, in vitro antiproliferatif aktivitesini değerlendirmektir. Bu amaçla ekstraktların 17 farklı fenolik madde içeriği HPLC ile belirlendi. Antiproliferatif aktivite ise, karaçiğer kanseri hücre hattı (Hep G2), osteosarkoma hücre hattı (U-2 OS) ve sağlıklı fare fibroblast hücre (L-929) hücre hatlarına karşı MTT testi ile tayin edildi. Ekstraktlarda en fazla miktarda askorbik asit belirlendi. HNM ekstraktı içerik yönünden HNS ekstraktından daha zengindi.Antiproliferatif aktivite sonuçlarına göre en güçlü etkiyi HNM ekstraktı, özellikle Hep G2’ye karşı gösterdi. Genel olarak değerlendirildiğinde HNM ekstraktının fenolik madde içeriği ve antiproliferatif aktivite bakımından daha iyi olduğu belirlendi. Bitki ekstraktlarının aktif bileşenlerinin izole edilerek daha fazla in vivo ve in vitro çalışmaların yapılması önem arz etmektedir.

Hyoscyamus niger L. of Medical Importance: Phenolic Substance Content and in vitro Antiproliferative Activities

Medically important plants represent one of the most important sources of drugs in the treatment of various diseases, including cancer, due to their rich components. The aim of this study was to investigate the content of 17 different phenolic substances of methanol (HNM) and water (HNS) extracts of the aerial parts of the Hyoscyamus niger plant and to evaluate the in vitro antiproliferative activity. For this purpose, the content of 17 different phenolic substances of the extracts was determined by HPLC. Antiproliferative activity was determined by MTT assay against osteosarcoma cell line (U-2 OS), liver cancer cell line (Hep G2) and healthy mouse fibroblast cell (L-929) cell lines. The highest amount of ascorbic acid was determined in the extracts. HNM extract was richer in content than HNS extract. According to the antiproliferative activity results, HNM extract showed the strongest effect, especially against Hep G2. When evaluated in general, it was determined that HNM extract was better in terms of phenolic content and antiproliferative activity. By isolating the active components of plant extracts. It is important to conduct more in vivo and in vitro studies.

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[1] Tanker N., Koyuncu M., Coşkun M. (2007). Solanaceae, Farmasötik Botanik, Üniversitesi Eczacılık Fakültesi Yayınları, Ankara.
[2] Davis P.H. (1975). "Flora of Turkey and the East Aegean Islands". Edinburgh University Press, Edinburgh.
[3] Kennedy David O. (2014). "The Deliriants - The Nightshade (Solanaceae) Family". Plants and the Human Brain. pp. 131–137, Oxford University Press, New York.
[4] Siegel R., Naishadham D., Jemal A. (2012). Cancer statistics. CA: A Cancer Journal for Clinicians. 62:10-29.
[5] Huang T., Gong W.H., Li X.C., Zou C.P., Jiang G.J., Li X.H., Feng D.P. (2012). Induction of apoptosis by a combination of paclitaxel and carboplatin in the presence of hyperthermia. Asian Pacific Journal of Cancer Prevention, 13:81-85.
[6] Park H.J., Kim M.J., Ha E., Chung J.H. (2008). Apoptotic effect of hesperidin through caspase3 activation in human colon cancer cells, SNU-C4. Phytomedicine, 15:147-151.
[7] Uzun M., Kaya A. (2019). Traditional medicinal plants used for oral and dental diseases in Turkey. Biological Diversity and Conservation, 12(1): 138-148.
[8] Mateus L., Cherkaoui S., Christen P., Veuthey J.L. (1998). Capillary electrophoresis for the analysis of tropane alkaloids: Pharmaceutical and phytochemical applications. Journal of Pharmacetical and Biomedical Analysis, 18: 815-825.
[9] Aljibouri A.M.J., Al-samarraei K.W., Abd A.S., Magee D.M., Ali A.J.A. (2012). Alkaloids production from callus of Hyoscyamus niger L. In vitro. Journal of Life Sciences, 6(8):874.
[10] Tuncel N.B.,Yılmaz N. (2010). Kaz Dağları’ndan toplanan bazı bitkilerin fenolik asit kompozisyonlarının yüksek performanslı sıvı kromatografisi ile belirlenmesi. Akademik Gıda, 8(3):18-23.
[11] Stavric B. (1994) .Role of chemopreventers in human diet. Clinical Biochemistry, 27(5):319-332.
[12] Begum, A.S. (2010). Bioactive non-alkaloidal secondary metabolites of Hyoscyamus niger Linn. seeds: A review. Research Journal of Seed Science, 3:210–217.
[13] Uğur D., Güneş H., Güneş F., Mammadov R. (2017). Cytotoxic activities of certain medicinal plants on different cancer cell lines. Turkish Journal of Pharmaceutical Sciences, 14(3):222-230.
[14] Alan Y., Yılmaz N. (2019). Phenolic substance contents and biological activities of Verbascum Insulare Boıss. & Heldr. Extracts, Farmacia, 67(4):641-647.
[15] Savci A, Kocpinar E.F., Alan Y., Kursat M. (2020). Antioxidant, antimicrobial, and DNA protection activities of some Tanacetum species and phenolic richness in their ethanolic extracts. International Food Research Journal, 27(1):160-170.
[16] Alkış E., Keleştemür Ü., Alan Y, Turan N., Buldurun K., (2021). Cobalt and ruthenium complexes with pyrimidine based schiff.base: Synthesis, characterization, anticancer activities and electrochemotherapy efficiency. Journal of Molecular Structure 1226, 129402.
[17] Frohne D., Pfander H.J. (1983). A Colour Atlas of Poisonous Plants; Wolfe Publishing: London, UK.
[18] Ghorbanpour M., Hatami M., Hatami M. (2015). Activating antioxidant enzymes, hyoscyamine and scopolamine biosynthesis of Hyoscyamus niger L. plants with nano-sized titanium dioxide and bulk application. Acta agriculturae Slovenica, 105:23–32.
[19] Akkol E.K., Ilhan M., Kozan E., Tuğçe F., Dereli G., Sak M., Sobarzo-Sánchez E. (2020). Insecticidal Activity of Hyoscyamus niger L. On Lucilia sericata Causing Myiasis. Plants, 9, 655.
[20] Ma C.Y., Liu W.K., Che C.T. (2002). Lignanamides and nonalkaloidal components of Hyoscyamus niger seeds. Journal of Natural Products , 65 (2): 206-209.
[21] Hajipoor K., Sani A.M., Mohammad A. (2015). In vitro antioxidant activity and phenolic profile of Hyoscyamus niger. IJBPAS, 4(7): 4882-4890. [22] Jassbi A.R., Miri R., Masroorbabanari M., Asadollahi M., Attarroshan M., Baldwin I T. (2014). HPLC-DAD-ESIMS analyses of Hyoscyamus niger and H. reticulatus for their antioxidant constituents. Austin Chromatog,r 1(5):1022.
[23] Xifeng W.U., Jian G., Spitz M. (2007). Mutagen sensitivity; Genetic predisposition factor for cancer. Cancer Research, 67:3493 – 3495.
[24] Bassem S.E., Walid F., Khaled M., Salwa M., May E. (2010). Screening of natural products for therapeutic activity against solid tumors. Indian Journal of Experimental Biology, 48:258 – 284.
[25] Yahia M.,Yahia M., Benhouda A., Haba H. (2018). New Biological Anticancer Activities of Atropine Isolated From Algerian Hyoscyamus albus’s Leaves. Pharmacologyonline, 3, 286-296.
[26] A. O. Ismeel, “Cytogenetic and cytotoxic studies on the effect of phytoinvestigated active compounds of Hyoscyamus niger (in vivo and ex vivo) ”, PhD thesis, Al-Nahrain University- College of Science, Iraq, 2011.
[27] K. Murota and J. Terao, “Antioxidative flavonoid Q: implication of its intestinal absorption and metabolism”, Arch. Biochem. Biophys., vol. 417, pp. 12–17, 2003.
[28] Y. Massinissa, Y. Mouloud and A. Benhouda, “Antitumor Activity of Methanolic Fractions Extracted From the Aerial Part of Algerian Hyoscyamus albus and apoptotic cell aspect screening”, Ind. J. of Pharmac. Educa. and Res., vol. 52, pp. 1-21, 2018.
[29] H. Servi, B. Eren Keskin, S. Çelik, U. Budak and B. Kababıyık, “Essential oil and fatty acid composition of endemic Gypsophila laricina Schreb. from Turkey”, Turk J Pharm Sci., vol. 16, no. 2, pp. 220-226, 2019.