Öz This study was performed to detect viral pathogens between the years of 2003-2004 in potato growing areas of Niğde and Nevşehir provinces by using serological and biological methods. In the course of survey, leaf and tuber samples were collected randomly from symptomatic and non-symptomatic plants. These samples tested for PVX (Potato X potexvirus), PVY (Potato Y potyvirus), PVS (Potato S carlavirus), PVA (Potato A potyvirus), PVM (Potato M carlavirus) and PLRV (Potato leafroll luteovirus) by using DAS-ELISA and mechanical inoculation. PVY, PVS, PVX, PVA and PLRV viruses were detected in both potato leaves and tubers by using DAS-ELISA method in Niğde and Nevşehir. These viruses were detected as single or mix infections. Beside single infections like PVX, PVY, PVS and PLRV, mix infections like PVY+PVS, PVY+PLRV, PVY+PVS+PLRV, PVS+PVA and PVY+PVA were also encountered as complex infections. When PVS inoculated to Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum cv. Maden, N.t. cv White Burley, N.t. cv. Xanthi, N. glutinosa, Capsicum annuum, Datura stramonium test plants, some symptoms such as; mosaic, with bubble mosaic, mottling, vein clearing, vein banding, leaf distortion, chlorotic and necrotic local lesion were observed on leaves. The over all disease incidence was calculated as 32.35 % for 2003 and 34.05 % for the year 2004.