Filiz ÜNAL, Harun BAYRAKTAR, A. Faik YILDIRIM, Kadir AKAN, F. Sara DOLAR

Kayseri, Kırşehir, Nevşehir ve Aksaray illeri buğday ekim alanlarındaki Rhizoctonia tür ve anastomosis gruplarının belirlenmesi

Kayseri, Kırşehir, Nevşehir ve Aksaray illerine ait buğday üretim alanlarında Rhizoctonia tür ve anastomosis grupları ile patojenisitelerini belirlemek amacıyla 2011 ve 2012 yılı üretim sezonunda survey yapılmış, kök ve kök boğazında lezyon ile cüceleşme belirtisi gözlenen bitkilerden ve rizosfer toprağından 320 bitki ve toprak örneği toplanmıştır. Bitkilerden yapılan izolasyonlar sonucunda 45, topraktan yapılan izolasyonlar sonucunda ise 8 olmak üzere toplam 53 adet Rhizoctonia izolatı elde edilmiştir. Yapılan klasik teşhis çalışmaları ve DNA sekans analizleri sonucunda elde edilen multinükleat izolatların Rhizoctonia solani AG 3, AG 4 HG II, AG 5 ve Waitea circinata var. circinata anastomosis gruplarına ait olduğu belirlenmiştir. Binükleat Rhizoctonia izolatları ise AG I ve AG K olarak tespit edilmiştir. Yapılan patojenisite testleri sonucunda AG 4 HG II ve Waitea circinata var. circinata grupları buğdayda patojen bulunmuş ve en virulent grubun ise AG 4 HG II olduğu belirlenmiştir.

Anahtar Kelimeler:

Rhizoctonia spp., anastomosis grup; moleküler teşhis; buğday

Determination of Rhizoctonia species and anastomosis groups in wheat production areas in Kayseri, Kırşehir, Nevşehir and Aksaray provinces

For the purpose of determination of the pathogenicity, anastomosis groups and species of Rhizoctonia in wheat production areas in Kayseri, Kirsehir, Nevsehir and Aksaray provinces, survey were conducted in the 2011 and 2012 growing seasons, 320 plant samples were collected from the plants with lesions on root and crown, stunting plants and also 320 samples collected from rhizosphere soil. Totally 53 Rhizoctonia isolates were obtained, 45 isolates as a result of isolations performed from the plants and 8 isolates from the soil. It was determined that the multinucleate isolates obtained as a result of conventional identification studies and DNA sequence analysis belonged to Rhizoctonia solani AG 3, AG 4 HG II, AG 5 and Waitea circinata var. circinata anastomosis groups. Binucleate Rhizoctonia isolates were detected as AG I and AG K. As a result of pathogenicity tests, AG 4 HG II and Waitea circinata var circinata groups were found to be pathogen on wheat and it was determined that the most virulent group is AG 4 HG II.

Keywords:

Rhizoctonia spp., anastomosis group; molecular identification; wheat,

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