Taner ÖZCAN, Özlem ÇETİN, Mustafa ÇELİK, Fatih ALBAYRAK

Opopanax ve Crenosciadium cinsleri arasındaki filogenetik ilişkinin çekirdek ve kloroplast DNA dizisi verilerinin kullanılarak belirlenmesi

Opopanax ve Crenosciadium üzerine filogenetik bir çalışma, cinslerin sınırlarını ve cinsler arası ilişkilerini aydınlatmak için yapıldı. Çekirdek DNA’sından ITS, kloroplast DNA’sından matK ve trnL-F'’den elde edilen dizi verileri, filogenetik ilişkilerini belirlemek için kullanıldı. Tüm bölgelerin Polimeraz Zincir Reaksiyonu (PCR) amplifikasyonları evrensel primerler kullanılarak yapıldı. Maksimum Olabilirlik (ML) ağaçları, PAUP* yazılımı kullanılarak oluşturuldu, ITS dizilerinin nod yaşlarını gösteren bir diğer filogenetik ağacı MrBayes programından elde edildi ve Komşu Ağ Diyagramları, Splits Tree Programı kullanılarak oluşturuldu. Sonuçlarımız, Smyrniopsis ve Opopanax'ın Petroedmondia syriaca, Magydaris panacifolia ve Crenosciadium siifolium ile güçlü bir şekilde yakın ilişkili olduğunu açıkça gösterdi.

Defining phylogenetic relationship between the genera Opopanax and Crenosciadium using nuclear and chloroplast DNA sequence data

A phylogenetic study of the Opopanax and Crenosciadium was conducted to elucidate their circumscription and infrageneric relationships. Sequence data from nrDNA ITS, cpDNA matK and trnL-F were used to determine their phylogenetic relationship. Polymerase Chain Reaction (PCR) amplifications of all regions were performed using universal primers. Maximum Likelihood (ML) trees were constructed using PAUP* software, another phylogenetic tree of ITS sequences showing node ages was obtained from MrBayes programme and Neighbour Net Diagrams were constructed using Splits Tree programme. Our results clearly showed that Smyrniopsis and Opopanax are allied strongly with Petroedmondia syriaca, Magydaris panacifolia, and Crenosciadium siifolium.

Keywords:

Opopanax, Crenosciadium, Apiaceae, Phylogeny,

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