Jelatin esaslı biyosorbentler ile albumin ve IgG uzaklaştırma uygulamaları

Hastalığa özgü proteinlerin belirlenmesinde kan plazmasındaki en yüksek derişimdeki albümin ve IgG’nin uzaklaştırılması, tanı ve tedavi bakımından büyük önem taşımaktadır. Bu çalışmada afinite kromatografisinde destek malzemesi olarak kullanılacak biyosorbentin hazırlanması amaçlanmıştır. Küresel biyosorbentler elde etmek için, jelatin biyosorbentler, su içinde yağ emülsiyon yöntemi ile hazırlanmış ve biyofonksiyonel çapraz bağlayıcı olarak glutaraldehit ile stabilize edilmiştir. Hazırlanan jelatin biyosorbentler, optik mikrograf ve SEM (taramalı elektron mikroskobu) ile karakterizasyon çalışmaları yapılmıştır. Optimal koşullar altında hazırlanan selüloz biyosorbentlerin boy/boy dağılımının ortalama 3-10 µm olduğu tespit edilmiştir. Sonuçlar, su ortamındaki albüminin % 85,7'si ve IgG'nin% 79'unun jelatin biyosorbentlerle başarıyla uzaklaştırıldığını ortaya koymuştur. Düşük konsantrasyonlu proteinlerin erken teşhisinde yüksek konsantrasyonlardaki proteinlerden albümin ve IgG'nin uzaklaştırılmasında jelatin biyosorbentlerin çok etkili olacağı düşünülmektedir.

Albumin and IgG removal applications with gelatin based biosorbents

Removal of the highest amount of albumin and IgG in the blood plasma for the determination of disease-specific proteins is of great importance in the field of diagnosis and treatment. In this study it is aimed to prepare the biosorbent to be used as a support material in affinity chromatography. Gelatin biosorbents were prepared by water in oil emulsion method and stabilized with glutaraldehyde as a biofunctional crosslinker to obtain spherical biosorbents. Prepared gelatin biosorbents were characterized by optical micrograph and SEM (scanning electron microcope). It was determined that the size/size distribution of the cellulose biosorbents prepared under optimal conditions was on average 3-10 µm. The results revealed that 85,7% of albumin and 79% of IgG in the aqua medium was successfully removed with gelatin biosorbents. It is thought that gelatin biosorbents will be very effective in removing albumin and IgG from high concentrations of proteins in the early diagnosis of low concentration proteins.

Keywords:

Gelatin, biosorbent, albumin, IgG bioaffinity chromatography, proteomic,

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