ELİF SİNEM İPLİK, Resul KAHRAMAN, BARIŞ ERTUĞRUL, Gonca CANDAN, Arzu ERGEN, BEDİA ÇAKMAKOĞLU

The role of COX-2 gene variants on the disease mechanism of inflammatory bowel disease in a Turkish population

Aim: Inflammatory bowel disease has two major types: Crohn’s disease and ulcerative colitis that occur in the gastrointestinal tract with unknown etiology. COX-2 has important role on carcinogenesis process including colon cancer supporting the tumor growth. COX-2 was also known due to its ability to change homeostasis on colonic mucosa in inflammatory cells on patients who have inflammatory bowel disease. In this study, we have aimed to find a linkage between inflammatory bowel disease and COX-2 in a Turkish population. Methods:A total of 106 patients,42 with Crohn’s disease and 64 with ulcerative colitis and 121 healthy control subjects were included the study. Gene variants of COX-2-765G→C and COX-2-1195A→G were analyzed by polymerase chain reaction and restriction fragment length polymorphism techniques. Results: The results demonstrated that COX-2-1195A→G gene variants AA carriers were statistically found in high level on patients with both ulcerative colitis (p=0.001) and Crohn’s disease (p=0.008). In contrast, AG genotype and G carriers were statistically found higher in control group (Crohn’s disease, p=0.005 for AG and p= 0.008 for G; ulcerative colitis, p=0.001 for AG and p=0.001 for G). Conclusion: In this research, we have observed important and questionable results between inflammatory bowel disease and COX-2, especially COX-2-1195A→G gene variants AA carriers in a Turkish population. Researches need to focus on their local roles on inflammatory bowel disease pathogenesis with large sample size.

Türk toplumundaki inflamatuar bağırsak hastalığının hastalık mekanizmasında COX-2 gen varyantlarının rolü

Amaç: İnflamatuar bağırsak hastalığı, gastrointestinal sistemde etiolojisi tam olarak bilinmeyen bir hastalık grubu olup, Crohn hastalığı ve ülseratif kolit olmak üzere iki önemli tipe ayrılmaktadır. COX-2 kolon kanserinin de dahil olduğu karsinogenez prosesinde rolü olan ve tümör gelişimine katkı sağlayan bir mediatördür. Aynı zamanda, inflamasyon meydana geldiğinde kolonik mukoza üzerindeki stabiliteyi değiştirdiği bilinmektedir. Bu amaçla, çalışmamızda inflamatuar barsak hastalığı ile COX-2 ilişkisinin Türk kökenli kişiler üzerinde araştırılması amaçlanmıştır. Yöntemler: Çalışmaya 42 Crohn ve 64 ülseratif kolitli hasta olmak üzere toplam 106 hasta) ve 121 sağlıklı kontrol dahil edilmiştir. COX-2 -765G→C ve COX-2 -1195A→G gen varyantları polimeraz zincir reaksiyonu ve restriksiyon parça uzunluk polimorfizmi teknikleri kullanılarak analiz edilmiştir. Bulgular: COX-2-1195A→G gen varyantı AA taşıyıcılarının istatistiksel olarak hasta grubunda (Crohn hastalığı için p=0,008 ve ülseratif kolit için p=0,001) kontrol grubuna göre yüksek bulunmuştur. Buna karşılık olarak AG genotipi ve G taşıyıcıları da kontrol grubunda anlamlı olarak yüksek bulunmuştur (Crohn hastalığı: p=0,005 AG için ve p= 0,008 G için; ülseratif kolit: p=0,001 AG için ve p=0,001G için). Sonuç: Çalışmada Türk kökenli kişiler üzerinde, inflamatuar bağırsak hastalığı ve COX-2 arasındaki ilişkiye dair COX-2-1195A→G AA taşıyıcıları gibi önemli ve araştırılması gerekli soruları beraberinde getiren bulgular elde edilmiştir. Hasta sayısının arttırılarak ek çalışmalar ile hastalık patogonezindeki rolünün tam olarak araştırılması gerektiği düşünülmektedir.

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