SELAHATTİN ATMACA, TUNCER ÖZEKİNCİ, Salim YAKUT, NEZAHAT AKPOLAT, Kadri GÜL

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SERRATIA TÜRLERİNİN İDENTİFİKASYONU, KLİNİK DAĞILIMI, ANTİBİYOTİK DUYARLILIĞI

Serratia spp. özellikle yenidoğan, çocuk ve diğer yoğun bakım üniteleri başta olmak üzere hastane infeksiyonu etkenleri arasında önemli yeri olan bir bakteri grubudur. Çalışmada klinik ve polikliniklerden gönderilen farklı örneklerden izole edilen Serratia marcescens ve diğer Serratia türlerinin klinik kökenleri ve antibiyotik duyarlılık sonuçlarının tartışılması amaçlanmıştır. İki yıllık bir dönem içerisinde kliniklerden ve poliklinikten gönderilen farklı materyallerden infeksiyon etkeni olarak izole edilen Serratia suşlarının identifikasyonu MALDI-TOF-MS (Matrix-assisted laser desorption/ionization- time of flight- mass spectrometry, Bruker Daltonics, ABD), duyarlılık testleri ise Phoenix UNMIC-401/ ID Paneli ve NMIC-400/ID Paneli (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md, ABD) ile yapılmıştır. İzole edilen 105 Serratia suşunun 17’si (% 16) poliklinik, 88’i (% 84) klinik ve yoğun bakım hastalarından izole edilmiş olup 89 suş (% 84.7) S.marcescens, beş suş (% 4.7) Serratia ureilytica, dört suş (% 3.8) Serratia liquefaciens, birer suş da Serratia ficaria, Serratia fonticola ve Serratia plymuthica olarak tanımlanırken dört Serratia cinsine ait izolatın sistem tarafından tür identifikasyonu yapılamamıştır. Toplam örneklerin 51’i (% 49) yoğun bakım ünitelerinden gönderilmiş olup bu sayı içerisinde yenidoğan ve çocuk yoğun bakım ünitelerindeki yoğunluk (% 56.8) dikkat çekicidir. Sonuç olarak ciddi bir hastane enfeksiyonu etkeni olarak bilinen Serratia cinsi bakterilerin antibiyotik duyarlılık sonuçlarını sunarak bu bakteri cinsine dikkat çekmek istenmiştir. Serratia cinsi bakterilerin özellikle yoğun bakım hastalarında, yoğun bakım üniteleri içinde ise önemli oranda çocuk yoğun bakım ünitelerinde enfeksiyonlara yol açtıkları ve daha çok pnömoni, idrar yolu enfeksiyonları, sepsis ve yara yeri enfeksiyonlarına neden oldukları görülmektedir. Aynı zamanda çalışmamızda Serratia türlerine karşı sırayla amikasin (% 97), siprofloksasin (% 95), aztreonam (% 93) ve trimetoprim/sülfametoksazol (% 93) en etkili antibiyotikler olarak tespit edilmiştir.

Identification of Serratia Species, Clinical Distribution, Antibiotic Susceptibility

Serratia spp. is a group of bacteria that has an important place among nosocomial infections, especially neonates, children and other intensive care units. The aim of the study is to discuss the clinical origins and antibiotic susceptibility results of Serratia marcescens and other Serratia species isolated from different material specimens sent from inpatient and outpatient clinics. Identification of Serratia strains isolated from different materials for two years was performed by MALDI-TOF-MS (Matrix-assisted laser desorption/ionization- time of flight- mass spectrometry, Bruker Daltonics, USA) Susceptibility tests were done with Phoenix UNMIC-401 / ID Panel and NMI-400 / ID Panel (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md., USA). 17 (16 %) of 105 Serratia spp. strains were isolated from outpatient clinics, 88 (84 %) were isolated from clinical and intensive care patients. Of these, 89 (84.7 %) were S.marcescens, five (4.7 %) were Serratia ureilytica, four (3.8 %) were Serratia liquefaciens, one were Serratia ficaria, Serratia fonicola and, four of them could not be identified to species level by the system. Fifty one (49 %) of the total samples were sent from intensive care units and the tendency to neonatal and pediatric intensive care units (56.8 %) was remarkable. As a result, by presenting their antibiotic susceptibility results, we want to draw attention to this bacterial genus which are known as one of the agent of serious hospital infections. It is seen that Serratia spp. cause infections especially in intensive care units mostly in pediatric intensive care units and cause pneumonia, urinary tract infections, sepsis and wound infections. We have also identified amikacin (% 97), ciprofloxacin (% 95), aztreonam (% 93) and trimethoprim/sulfamethoxazole (% 93) as the most effective antibiotics against Serratia species in our hospital.
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ANKEM Dergisi-Cover
  • ISSN: 1301-3114
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1986
  • Yayıncı: Antibiyotik ve Kemoterapi Derneği
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