TÜRKİYE'DE DOĞAL OLARAK YETİŞEN VE AKTARLARDA SATILAN SUMAK (RHUS CORIARIA L.) MEYVELERİNİN YAĞ ASİDİ KOMPOZİSYONLARININ KİMYASAL KARAKTERİZASYONU VE ANTİMİKROBİYAL AKTİVİTESİ

Amaç: Bu çalışma Tunceli ve Siirt ilerinde doğal olarak yayılış gösteren örnekler ile aktarlarda toz ve tane olarak satılan R. coriaria L. örneklerinin yağ asiti bileşenleri ve antimikrobiyal özelliklerini karşılaştırmak amacı ile yapılmıştır.Gereç ve Yöntem: Tohumlar tartıldıktan ve toz haline getirildikten sonra soksalet kullanarak sabit yağları elde edilmiştir. Elde edilen sabit yağların, metilleme işlemi yapıldıktan sonra, GC ve GC/MS yöntemleriyle eş zamanlı olarak analizleri gerçekleştirilmiştir. Sabit yağ numunelerinin in vitro antimikrobiyal aktivite çalışmaları altı farklı Gram negatif ve Gram pozitif bakteri ve Candida albicans’a karşı EUCAST disk difüzyon ve CLSI mikrodilüsyon yöntemleri kullanılarak gerçekleştirilmiştir. Sonuç ve Tartışma: Tüm numunelerin ana yağ asidi bileşenleri, sırasıyla oleik asit (%42.2 - 43.3), linoleik asit (%25.2 - 28.5) ve palmitik asit (%18.4-221.5) olarak tanımlandı. Sabit yağların in vitro antimikrobiyal aktivitesi, Escherichia coli NRRL B-3008, Staphylococcus aureus ATCC 6538, S. epidermidis ATCC 14990, Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis NRRL B-A78 A78 gibi insan patojenik mikroorganizmalarına karşı gerçekleştirildi. Sonuçlar standart antimikrobiyal maddeler ile karşılaştırılmış ve MIC değerleri > 2,5 - 0.22 mg/ml belirlenmiştir. Sonuçların antimikrobiyal etkinlik açından kayda değer olduğu görülmüştür.

Anahtar Kelimeler:

Antimikrobial, CLSI, EUCAST, GC-FID, GC-MS, Rhus coriaria

CHEMICAL CHARACTERIZATION OF THE FATTY ACID COMPOSITIONS AND ANTMICROBIAL ACTIVITY OF SUMAC (RHUS CORIARIA L.) FRUITS, GROWING NATURALLY IN TURKEY AND SOLD IN HERBALIST MARKETS

ABSTRACT Objective: The aim of this study was to compare the fatty acid components and antimicrobial properties of R. coriaria L. samples which were sold as powder and grains tjat show natural distribution in Tunceli and Siirt. Material and Method: After the seeds were weighed and powdered, fixed oils were obtained by using soxhalet apparatus. Obtained fixed oils were analyzed by GC and GC / MS methods after methylation process. In vitro antimicrobial activity studies of the samples were performed using six different Gram negative and Gram positive bacteria and Candida albicans using EUCAST disc diffusion and CLSI microdilution methods. Results and Discussion: The main fatty acid components of all samples were determined as oleic acid (42.2 - 43.3%), linoleic acid (25.2 - 28.5%) and palmitic acid (18.4-221.5%), respectively. In vitro antimicrobial activity of fixed oils, such as Escherichia coli NRRL B-3008, Staphylococcus aureus ATCC 6538, S. epidermidis ATCC 14990, Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis NRRL B-A78 A78 Ausugusar ATC 27853. Results were compared with standard antimicrobial agents and MIC values> 2.5 - 0.22 mg / mL. The results were found to be significant in terms of antimicrobial efficacy.

Keywords:

Rhus coriaria, GC-FID, GC-MS, EUCAST, CLSI, antimicrobial,

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