Hacı BALKAYA, Semih KÜÇÜKGÜÇLÜ, Necati GÖKMEN, Osman YILMAZ, Alper BAĞRIYANIK, Remzi ÖZAY, Emel SAĞIROĞLU

Termal ağrı modelinde intratekal ropivakainin analjezik etkilerine intratekal midazolamın etkisi

Amaç: Bu çalışmada, sıçanlarda intratekal verilen ropivakaine, midazolam eklenmesinin analjezik ve spinal koradaki histopatolojik etkisinin araştırılması amaçlandı. Yöntem: Grup Sham: tüm cerrahi işlemler yapıldı, ancak kateter takılmadı; Grup S: kateterden 10 $mu$ %0.9 serum fizyolojik solüsyonu verildi; Grup R: kateterden 10 $mu$ 50 $mu$ ropivakain verildi; Grup M: kateterden 10 $mu$ 20 pıg'lık midazolam verildi; Grup MR: kateterden 10 uL midazolam, ropivakain karışımı (20 pıg midazolam, 50 pıg ropivakain olacak şekilde) verildi. Kateter tüm gruplara ilaç verildikten sonra 10 $mu$ serum fizyolojik ile yıkandı. Tail-flick ve rotarod testi ilaç uygulamalarından sonraki 10, 20, 30, 45, 60 ve 90. dakikalarda yapıldı. Sıçanların spinal kordundan alınan kesitler histopatolojik olarak değerlendirildi. Bulgular: Tail-flick Sesti sonucunda; Grup S ile Grup R, Grup M ve Grup MR karşılaştırıldığında; Grup R, Grup M ve Grup MR'da tail-flick ortalama değerleri anlamlı yüksek bulundu (p

Anahtar Kelimeler:

Analjezi, Enjeksiyon, spinal, Erkek, Midazolam, Ağrı, Sıçan, Wistar, Modeller, hayvan

The influence of intrathecally administered midazolam on the analgesic effects of intrathecally administered ropivacaine for thermally induced pain

Objective: This study was aimed to investigate whether the addition of midazolam to the intrathecal administration of ropivacaine in rats affects analgesic activity or has a histopathological effect on the spinal cord. Methods: Group Sham: All surgical processes were carried out except catheter insertion; Group S: 10 $mu$ of 0,9 % saline was administered through the catheter; Group R: 10 $mu$ ropivacaine including 50 $mu$ was administered through the catheter; Group M: 10 pıL midazolam including 20 ug was administered through the catheter; Group MR: 10 $mu$ of midazolam and ropivacaine mixture (with 20 pig midazolam and 50 $mu$ ropivacaine) was administered through the catheter. Tail-flick and rotarod tests were performed at 10, 20, 30, 45, 60, and 90 mins after drug injection. The cross-sections obtained from the spinal cords of the rats were histopathologically evaluated. Results: The tail flick test mean values in group R, group M and group MR were significantly longer than in group S (p<0.05). In the comparison of group R with groups M and MR, the mean values of group MR were found to be significantly long (p<0.05). The rotarod test mean values of group M and group MR were found to be significantly low (p<0.05). No statistical histopathologic significance was found in any of the groups (p>0.05). Conclusion: The analgesic property of ropivacaine when administered intrathecally was augmented by intrathecally administered midazolam without producing any histopathological neurotoxicity.

Keywords:

Analgesia, Injections, Spinal, Male, Midazolam, Pain, Rats, Wistar, Models, Animal,

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