Hüseyin C. TURGUT, İ. Aydın ERDEN, Seda B. AKINCI, Fatma SARICAOĞLU, Sevgen ÖNDER, Kamer KILINÇ, Hüsnü Can BAŞER, Ülkü AYPAR

Asetaminofen'in neden olduğu hepatotoksisitede karvakrol'ün etkisinin değerlendirilmesi ve n-asetil sistein ile karşılaştırılması

Amaç: Asetaminofen (APAP), terapötik dozlarda yan etkisi az bir analjeziktir. Bununla birlikte hepatotoksisite ile karşılaşılabilmektedir. Karvakrolün karaciğer rejenerasyonu, iskemi reperfüzyon hasarı üzerinde yararlı etkileri gösterilmiştir. Bu çalışmada asetaminofene bağlı karaciğer toksisitesinde karvakrolün etkisi araştırılarak, N-asetil sistein (NAS) ile karşılaştırılması amaçlanmıştır. Gereç Yöntem: Çalışmaya 36 adet Wistar Albino rat (6 grup) alınmıştır. Birinci grup APAP (1 g kg-1 oral) + karvakrol (karvakrol (73 mg kg-1) grubu, ikinci grup serum fizyolojik (SF) (1 mL kg-1 % 0,9luk NaCl intraperitoneal) grubu, üçüncü grup karvakrol (73 mg kg-1) grubu, dördüncü grup APAP + N-asetilsistein (1.2 g kg-1 ip) grubu, beşinci grup APAP (1 g kg-1 oral) grubu, altıncı grup N-asetilsistein (1.2 g kg-1 ip) grubu olarak düzenlendi.Yirmi dördüncü saatin sonunda serum alanin amino transferaz (ALT), aspartat amino transferaz (AST) ve alkalen fosfataz (ALP) düzeyleri ölçülerek histopatolojik değerlendirme yapıldı. Bulgular: Kontrol grubuna göre; APAP verilen grup, NAS verilen grup ve APAP+NAS verilen grupta anlamlı olarak yüksek inflamasyon tespit edildi. (p

Assesment of carvacrol effects on acetamınophen related hepatotoxıcıty and and ıts comparıson wıth n-acetyl cysteın

Objective: Acetaminophen is an analgesics agent with little side effects in range of therapeutic doses while hepatotoxicity is a common consequence of acetaminophen overdose. There has been beneficial effects of carvarcol on hepatic regeneration and ischemi-reperfusion injury. In this study we have aimed to examine the effects of carvacrol on acetaminophen induced hepatotoxiciy and compare it with N-acetyl cysteine (NAS). Material-Methods: Thirty six Wistar Albino rats have been used for the study (6 groups). In group 1 animals recieved carvacrol of 73 mg kg-1 intraperitoneally after receiving 1gr kg-1 APAP orally. Animals in control group received saline of 1 ml kg-1 orally. In group 3 animals recieved carvacrol of 73 mg kg-1 intraperitoneally. In group 4 animals recieved 1gr kg-1 of APAP orally followed by 1.2 gr kg-1 of NAC intraperitoneally. In group 5 animals received APAP of 1 gr kg-1 orally. In group 6 animals received NAC of 1.2 gr kg-1 intraperitoneally. Alanine amino transferase (ALT), aspartat amino transferase (AST) and alkaline phospatase (ALP) levels were measured after 24 hours, and histopathological examination was made. Results: Inflammation in APAP treated group, N-acetyl cysteine treated group and APAP+NAC group was remarkably high (p<0.05). Serum levels of ALT and AST were found to be higher in carvacrol treated group than APAP treated group and APAP+NAC treated group (p<0.05). Conclusion: Our study indicates that carvacrol can not provide sufficent protective effect, and neither carvacrol nor N-acetyl cysteine is superior to each other in protecting hepatocytes from acetaminophen induced hepatotoxicity. Carvacrol has no destructing effect on hepatic tissue according to histopathological findings, however, this result has been debatable due to the significant increase in carvacrol related aminotransferase levels.

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