Anestezi Çalışanlarında Çalışma Ortamından Uzaklaşmanın Oksidatif Stres Düzeylerine Etkisinin Dinamik Tiyol Disülfit Dengesi ile Değerlendirilmesi
Amaç: Atık gaz maruziyeti ve mesleki stres nedeniyle anestezi çalışanlarında oksidatif stres artmaktadır. Çalışmamızda, anestezi çalışanlarında minimum 1 hafta anestezi pratiğinden uzaklaşılmasının, oksidatif stres üzerine etkilerinin tiyol disülfit dengesiyle incelenmesi amaçlandı. Yöntem: Çalışmaya ameliyat odasında anestezi uygulamalarında aktif çalışan ve en az 1 hafta izne çıkmayı planlayan anesteziyoloji uzmanı, uzmanlık öğrencisi ve anestezi teknisyenleri arasından toplam 29 gönüllü dâhil edildi. Demografik verileri, çalışma süreleri ve görevleri kaydedildi. Gönüllülerden izne ayrılmadan önceki gün ve izinden döndüğü gün kan alınarak dinamik tiyol disülfit dengesi çalışıldı. İzin sonrası nativ ve total tiyol düzeylerinde artma ve disülfidde azalma oksidatif stres düzeyinde azalma olarak değerlendirildi. Veriler t-testi, tek yönlü varyans analizi, eşleştirilmiş t-testi ile değerlendirildi, p
Evaluating the Effect of Being Away From Work Environment on Oxidative Stress Levels with Dynamic Thiol-Disulfide Homeostasis Among Anesthesiology Professionals
Objective: Exposure to anesthetic gases and occupational stress lead to increased levels of oxidative stress in anesthesiology professionals. Our study aims to investigate the effects of being away from anesthetic practice on oxidative stress with thiol-disulfide balance in anesthesiology professionals. Method: The study included a total of 29 volunteers among anesthesiologists, residents and technicians working in anesthesiology practice and planning to take a leave lasting at least for a week. Demographic data, working times and duties of them were recorded. Blood samples were taken from the participants on the day before they went on leave and on the day they returned, to study dynamic thiol-disulfide homeostasis. Increased native and total thiol levels and decreased disulfide levels after returning were evaluated as a decrease in oxidative stress. The data were assessed with a t-test, a one-way analysis of variance, a paired t-test; and p
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- 1. Lee YM, Song BC, Yeu KJ. Impact of Volatile Anesthetics on Oxidative Stress and Inflammation. Biomed Res Int. 2015;2015:242709. https://doi.org/10.1155/2015/242709
- 2. Malekirad AA, Ranjbar A, Rahzani K, et al. Oxidative stress in operating room personnel: occupational exposure to anesthetic gases. Hum Exp Toxicol. 2005;24:597-601. https://doi.org/10.1191/0960327105ht565oa
- 3. Kozanhan B, Inanlı I, Deniz CD, et al. Dynamic thiol disulphide homeostasis in operating theater personnel exposed to anesthetic gases. Am J Ind Med. 2017;60:1003-09. https://doi.org/10.1002/ajim.22764
- 4. Dinc ME, Ulusoy S, Is A, et al. Thiol/disulphide homeostasis as a novel indicator of oxidative stress in sudden sensorineural hearing loss. J Laryngol Otol. 2016;130:447-52. https://doi.org/10.1017/S002221511600092X
- 5. Kundi H, Ates I, Kiziltunc E, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/ disulphide homeostasis. Am J Emerg Med. 2015;33:1567-71. https://doi.org/10.1016/j.ajem.2015.06.016
- 6. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014;47:326-32. https://doi.org/10.1016/j.clinbiochem.2014.09.026
- 7. Jafari A, Bargeshadi R, Jafari F, Mohebbi I, Hajaghazadeh M. Environmental and biological measurements of isoflurane and sevoflurane in operating room personnel. Int Arch Occup Environ Health. 2018;91:349-59. https://doi.org/10.1007/s00420-017-1287-y
- 8. Türkan H, Aydin A, Sayal A. Effect of volatile anesthetics on oxidative stress due to occupational exposure. World J Surg. 2005;29:540-42. https://doi.org/10.1007/s00268-004-7658-z
- 9. Costa Paes ER, Braz MG, Lima JT, et al. DNA damage and antioxidant status in medical residents occupationally exposed to waste anesthetic gases. Acta Cir Bras. 2014;29:280-86. https://doi.org/10.1590/S0102-86502014000400010
- 10. Jafari A, Jafari F, Mohebbi I. Effects of occupational exposure to trace levels of halogenated anesthetics on the liver, kidney, and oxidative stress parameters in operating room personnel. Toxin Reviews, 2018; 1-10. https://doi.org/10.1080/15569543.2018.1498898
- 11. Zanaty OM, El Metainy S, Abdelmaksoud R, Demerdash H, Aliaa DA, El Wafa HA. Occupational stress of anesthesia: Effects on aging. J Clin Anesth. 2017;39:159-64. https://doi.org/10.1016/j.jclinane.2017.03.047
- 12. Maes M, Galecki P, Chang YS, Berk M. A review on the oxidative and nitrosative stress (O&NS) pathways in major depression and their possible contribution to the (neuro)degenerative processes in that illness. Prog Neuropsychopharmacol Biol Psychiatry. 2011;35:676- 92. https://doi.org/10.1016/j.pnpbp.2010.05.004
- 13. Grigoruţă M, Martínez-Martínez A, Dagda RY, Dagda RK. Psychological Stress Phenocopies Brain Mitochondrial Dysfunction and Motor Deficits as Observed in a Parkinsonian Rat Model. Mol Neurobiol. 2020;57:1781-98. https://doi.org/10.1007/s12035-019-01838-9. [Epub ahead of print].
- 14. Krolow R, Arcego DM, Noschang C, Weis SN, Dalmaz C. Oxidative Imbalance and Anxiety Disorders. Curr Neuropharmacol. 2014;12:193-204. https://doi.org/10.2174/1570159X11666131120223530
- 15. Alves SL. A study of occupational stress, scope of practice, and collaboration in nurse anesthetists practicing in anesthesia care team settings. AANA J. 2005;73:443-52.
- 16. Gaszynska E, Stankiewicz-Rudnicki M, Szatko F, Wieczorek A, Gaszynski T. Life Satisfaction and WorkRelated Satisfaction among Anesthesiologists in Poland. ScientificWorldJournal. 2014;2014:601865, 9 pages. https://doi.org/10.1155/2014/601865. Epub 2014 Jun 9.
- 17. Casado A, López-Fernández ME. Age-correlated changes of the erythrocyte catalase activity in the Spanish population. Gerontology. 2003;49:251-4. https://doi.org/10.1159/000070406
- 18. Casado A, Castellanos A, López-Fernández ME, Ruíz R, Aroca CG, Noriega F. Relationship between oxidative and occupational stress and aging in nurses of an intensive care unit. Age (Dordr). 2008;30:229-36. https://doi.org/10.1007/s11357-008-9052-5