Normospermik bireylerde semen parametreleri ile nötrofil-lenfosit oranı (NLR) ve trombosit-lenfosit oranı (PLR) arasındaki ilişki

AMAÇ: Çeşitli hematolojik parametrelerin inflamatuar süreçlerde rol aldığı bilinmektedir. Son zamanlarda bu konuda en çok araştırılan parametrelerden olan nötrofil-lenfosit oranı (NLR) ve trombosit-lenfosit oranının (PLR) semen parametreleri ile ilişkisi hakkındaki bilgiler sınırlıdır. Biz bu çalışmamızda, normospermik bireylerde semen parametreleri ile NLR ve PLR arasındaki ilişkiyi araştırmayı amaçladık. GEREÇ ve YÖNTEM: Hastanemiz androloji laboratuarında Ocak 2017 – Aralık 2017 tarihleri arasında yapılmış semen analizleri retrospektif olarak tarandı. Kayıtlarda hemogram sonucu mevcut olan normospermik bireyler çalışmaya dahil edildi. Hemogram sonucu ile semen analizi arasında 6 aydan fazla süre olan olgular çalışma dışında bırakıldı. Hastaların trombosit sayısı, ortalama trombosit hacmi (MPV), nötrofil sayısı, lenfosit sayısı kayıt edildi ve NLR, PLR hesaplandı. Veriler semen parametreleri ile karşılaştırıldı. BULGULAR: Çalışmaya dahil edilen 90 hastanın yaş ortalaması 31,50±7,04 yıl idi. Hastaların hemogram parametrelerinde ortalama trombosit sayısı 237,89±59,58, MPV 7,53±0,91, nötrofil sayısı 4046±1277, lenfosit sayısı 2277±692; semen parametrelerinde semen hacmi 2,80±0,96 mL, konsantrasyon 69,08±31,87, toplam motil sperm sayısı 41,07±21,36, idi. Hemogram parametreleri, semen parametreleri ile karşılaştırıldığında aralarında istatistiksel olarak anlamlı korelasyon saptanmadı (Tablo 1). SONUÇ: Bu çalışmanın sonuçları, sağlıklı normospermik bireylerde semen parametreleri ile NLR ve PLR arasında ilişki olmadığını ve öngördürücü bir belirteç olarak kullanılamayacağını göstermektedir

The relationship between semen parameters vs. neutrophil-lymphocyte ratio and platelet-lymphocyte ratio in normospermic individuals

OBJECTIVE: It is known that various hematological parameters are involved in inflammatory processes. The data about relationship between semen parameters and neutrophil-lymphocyte ratio (NLR) or platelet-lymphocyte ratio (PLR), which are recently the most researched parameters in this regard, is limited. In this study, we aimed to investigate the relationship between semen parameters and NLR & PLR in normospermic individuals. MATRERIAL and METHODS: Semen analysis performed in our hospital andrology laboratory in between January 2017 and December 2017 were retrospectively reviewed. Normospermic individuals who had hemogram test in records, were included in the study. Subjects with a duration of more than 6 months between hemogram results and semen analysis were excluded from the study. Patients’ platelet count, mean platelet volume (MPV), neutrophil count, lymphocyte count were recorded and NLR, PLR were calculated. The data were compared with the semen parameters. RESULTS: Ninety patients included in the study and the mean age was 31.50±7.04 years. Mean platelet counts of the patients were 237.89±59.58, MPV 7.53±0.91, neutrophil count 4046±1277, lymphocyte count 2277±692; in semen parameters, semen volume 2.80±0.96 mL, concentration 69.08±31.87 and total motile sperm count was 41.07±21.36. There was no statistically significant correlation between hemogram parameters and semen parameters (Table 1). CONCLUSION: The results of this study show that there is no relationship between semen parameters and NLR&PLR in healthy normospermic individuals and NLR&PLR can not be used as a predictive marker.

Kaynakça

1. World Health Organization (WHO). WHO Manual for the Standardised Investigation and Diagnosis of the Infertile Couple, Cambridge: Cambridge University Press; 2000.

2. Fraczek M, Kurpisz M. Cytokines in the male reproductive tract and their role in infertility disorders. J Reprod Immunol 2015;108:98–104. [CrossRef]

3. Azenabor A, Ekun AO, Akinloye O. Impact of inflammation on male reproductive tract. J Reprod Infertil 2015;16:123–9. https://www.ncbi. nlm.nih.gov/pmc/articles/PMC4508350/

4. Aykan S, Canat L, Gönültaş S, Atalay HA, Altunrende F. Are There Relationships between Seminal Parameters and the Neutrophil-toLymphocyte Ratio or the Platelet-to-Lymphocyte Ratio? World J Mens Health 2017;35:51–6. [CrossRef]

5. Imtiaz F, Shafique K, Mirza SS, Ayoob Z, Vart P, Rao S. Neutrophil lymphocyte ratio as a measure of systemic inflammation in prevalent chronic diseases in Asian population. Int Arch Med 2012;5:2. [CrossRef]

6. World Health Organization (WHO). WHO Laboratory manual for the examination and processing of human semen, 5th ed. Geneva: WHO Department of Reproductive Health and Research; 2010.

7. Weidner W, Pilatz A, Diemer T, Schuppe HC, Rusz A, Wagenlehner F. Male urogenital infections: Impact of infection and inflammation on ejaculate parameters. World J Urol 2013;31:717–23. [CrossRef]

8. Haidl G, Allam JP, Schuppe HC. Chronic epididymitis: impact on semen parameters and therapeutic options. Andrologia 2008;40:92–6. [CrossRef]

9. Bachir BG, Jarvi K. Infectious, inflammatory, and immunologic conditions resulting in male infertility. Urol Clin North Am 2014;41:67– 81. [CrossRef]

10. Hedger MP. Testicular leukocytes: what are they doing? Rev Reprod 1997;2:38–47. [CrossRef]

11. Lampiao F, du Plessis SS. TNF-alpha and IL-6 affect human sperm function by elevating nitric oxide production. Reprod Biomed Online 2008;17:628–31. [CrossRef]

12. Zhang H, Yin Y, Wang G, Liu Z, Liu L, Sun F. Interleukin-6 disrupts bloodtestis barrier through inhibiting protein degradation or activating phosphorylated ERK in Sertoli cells. Sci Rep 2014;4:4260. [CrossRef]

13. Zalata A, Atwa A, El-Naser Badawy A, Aziz A, El-Baz R, Elhanbly S, Mostafa T. Tumor necrosis factor-α gene polymorphism relationship to seminal variables in infertile men. Urology 2013;81:962–6. [CrossRef]

14. Nallella KP, Allamaneni SS, Pasqualotto FF, Sharma RK, Thomas AJ, Agarwal A. Relationship of interleukin‐6 with semen characteristics and oxidative stress in patients with varicocele. Urology 2004;64:1010– 3. [CrossRef]

15. Hales DB. Testicular macrophage modulation of Leydig cell steroidogenesis. J Reprod Immunol 2002;57:3–18. [CrossRef]

16. Guthrie GJ, Charles KA, Roxburgh CS, Horgan PG, McMillan DC, Clarke SJ. The systemic inflammation-based neutrophil-lymphocyte ratio: experience in patients with cancer. Crit Rev Oncol Hematol 2013;88:218–30. [CrossRef]

17. Gürağaç A, Demirer Z. The neutrophil-to-lymphocyte ratio in clinical practice. Can Urol Assoc J 2016;10:141. [CrossRef]

18. Güneş M, Umul M, Altok M, Akyuz M, İşoğlu CS, Uruc F, et al. Predictive role of hematologic parameters in testicular torsion. Korean J Urol 2015;56:324–9. [CrossRef]

19. Zahorec R. Ratio of neutrophil to lymphocyte counts: rapid and simple parameter of systemic inflammation and stress in critically ill. Bratisl Lek Listy 2001;102:5–14.

20. Yucel C, Keskin MZ, Cakmak O, Ergani B, Kose C, Celik O, et al. Predictive value of pre-operative inflammation-based prognostic scores (neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and monocyte-to-eosinophil ratio) in testicular sperm extraction: a pilot study. Andrology 2017;5:1100–4. [CrossRef]

21. Ates E, Ucar M, Keskin MZ, Gokce A. Preoperative neutrophil-tolymphocyte ratio as a new prognostic predictor after microsurgical subinguinal varicocelectomy. Andrologia 2018:e13188. [CrossRef]

22. Kucukdurmaz F, Temiz Dogan N, Olmez C, Demir BT, Bilecan E, Resim S. Seminal parameters are not related to inflammatory markers (Neutrophilto-lymphocyte, platelet-to-lymphocyte and monocyte-toeosinophil ratios) and plasma vitamin D levels in infertile men. Androl Bul 2019;21:1−5. [CrossRef]

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