Bazı Ceviz (Juglans regia L.) Çeşitlerinin Antioksidant Aktiviteleri ve Fenolik Madde İçeriklerinin Belirlenmesi

Bu çalışmada Türkiye orijinli dört ceviz çeşidinden (Şebin, Bilecik, Kaman, Yalova-3) alınan meyvelerin fenolik madde içerikleri, antioksidan ve serbest radikal giderme aktiviteleri araştırılmıştır. Meyvelerdeki toplam fenolik madde miktarı 7.16-13.95 mg GAE g-1 arasında ve toplam flavonoid madde miktarı 0.73-1.11 mgQE g-1 arasında belirlenmiştir. Hem fenolik hem de flavonoid maddenin en yüksek miktarı Yalova-3 çeşidinde tespit edilmiştir. Ceviz meyvelerinin antioksidan aktivitesinin belirlenmesi amacıyla DPPH. (1,1-difenil-2-pikril-hidrazil), ABTS•+ (2,2´-azino-bis (3-etil benztiyoazolin -6-sülfonik asit) ve CUPRAC (cupric ion reducing antioxidant capacity) olmak üzere üç yöntem kullanılmıştır. Çeşitlerin serbest radikali süpürme aktiviteleri DPPH. yönteminde %28.35-39.44, ABTS•+ yönteminde %63.63-87.38 olarak tespit edilmiştir. Kuprik iyonlarını (Cu2+) indirgeme kapasitesi CUPRAC yöntemi ile 2.26-2.94 mMolTR g-1 arasında bulunmuştur. Meyve örneklerinin HPLC ile yapılan içerik analizlerinde bileşik olarak gallik asit, kateşin, kafeik asit, p-kumarik asit, trans ferulik asit ve orto-kumarik asit tespit edilmiştir. Tüm analizlerde ceviz çeşitleri arasındaki fark istatistiki olarak çok önemli bulunmuştur.

Determination of Antioxidant Activities and Phenolic Content of Some Walnut (Juglans regia L.) Cultivars

In this study phenolic contents of the four walnut cultivars (Şebin, Bilecik, Kaman, Yalova-3) originated from Turkey, antioxidant and radical scavenging activity was investigated. Total phenolic content has been determined 7.16-13.95 mg GAE g-1 and total flavonoid content between 0.73-1.11 mgQE g-1 in walnut varieties. The highest amount of both phenolic and flavonoid content were in the highest Yalova-3 cultivar. In order to determine the antioxidant activity of walnut fruit DPPH. (1,1-diphenyl-2-picril-hydrazyl), ABTS•+ (2,2´-azino-bis (3-ethyl benzthioazoline -6-sulfonic acid) and CUPRAC(cupric ion reducing antioxidant capacity) methods were used. Free radical sweeping activities of walnut varieties were determined as 28.35-39.44% by DPPH method and 63.63-87.38% by ABTS method. The capacity to reduce cupric ions (Cu2 +) was found between 2.26-2.94 mMolTR g-1 by CUPRAC method. In the analysis of walnut samples by HPLC, gallic acid, catechin, caffeic acid, p-coumaric acid, trans ferrulic acid and o-coumaric acid compounds were determined. In all analyzes the difference was found statistically very important between walnut varieties.

Keywords:

Walnut, Juglans.regia L., Phenolic Compound DPPH, ABTS, CUPRAC,

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