Başak BAYKARA, HÜSEYİN BURAK BAYKARA, Mehmet ATEŞ, İLKAY AKSU, Müge KİRAY, Ayşegül TAŞ, HİKMET GÜMÜŞ, CANER ÇETİNKAYA, Ali Rıza ŞİŞMAN, Durgül YILMAZ, Mehmet Nuri ARDA, NAZAN UYSAL HARZADIN

İmmatür sıçanlarda travmatik beyin hasarı ile ortaya çıkan anksiyetede serotonin ve serotonin 2A reseptörünün rolü

Amaç: Bu çalışmanın amacı immatür ratlarda, travmatik beyin hasarında ortaya çıkan anksiyetenin, prefrontalkortekste serotonin (5-HT) ve 5-HT 2A reseptörü ile ilişkisini araştırmaktır. Yöntem: Yedi günlük yavru ratlara travma-tik beyin hasarı modeli uygulandı ve beş gruba ayrıldı: 1-Sham; 2-Travmatik beyin hasarı (TBH) grubu; 3-Travmauygulanıp ardından 14 gün, 10 mg/kg essitalopram (seçici serotonin gerialım inhibitörü; SSRI) verilen grup(TBH+SSRI grubu); 4-Travma uygulanıp davranış deneylerinden 1 saat önce gastrik gavaj ile siproheptadin (non-spesifik serotonin reseptör antagonisti, A) 5 mg/kg verilen grup (TBH+A grubu); 5-Travma uygulanıp ardından 14gün essitalopram ve davranış deneylerinden 1 saat önce gastrik gavaj ile siproheptadin verilen grup (TBH+SSRI+Agrubu). Tüm gruplara yükseltilmiş +labirent testi ve açık alan testi uygulanmış, sonrasında kan kortikosteron, pre-frontal korteks doku serotonin ve 5-HT 2A reseptör miktarları ölçülmüştür. Prefrontal korteks nöron yoğunluğu değer-lendirilmiştir. Bulgular: TBH uygulanan grupta, açık alan testinde çevre hücrelerde geçirilen zaman ve yükseltilmiş+labirentin kapalı kollarında geçirilen zaman ve serum kortikosteron düzeyleri anksiyete ile doğru orantılı olarakartmış bulundu. Prefrontal kortekste nöron yoğunluğunda ise azalma gözlendi. SSRI tedavisi yükseltilmiş +labirenttestinde kapalı kollarda geçirilen süreyi azalttı. Serum kortikosteron düzeylerini düşürdü ve nöron yoğunluğunu artır-dı. Doku serotonin düzeyleri, TBH uygulanan tüm gruplarda, sham grubuna göre azalmış olarak saptandı. 5-HT 2Areseptör düzeyleri, TBH ve siproheptadin kullanan grupta yüksek bulundu. Sonuç: İmmatür ratlarda TBH’ye ikincilortaya çıkan anksiyetede SSRI’lar antianksiyete etki göstermiştir. (Anadolu Psikiyatri Derg 2019; 20(4):368-376)

The role of serotonin and serotonin 2A receptor in the anxiety due to traumatic brain injury in immature rats

Objective: The aim of this study is to investigate the relationship between anxiety due to traumatic brain injury (TBI) and prefrontal cortex serotonin (5-HT) and 5-HT 2A receptor in immature rats. Methods: Seven days old rats were subjected to traumatic brain injury model. They were divided into five groups. 1-Sham; 2-TBI group; 3-TBI followed by 14 days of administration of essitalopram (selective serotonin reuptake inhibitors; SSRI) (10 mg/kg) group (TBI+SSRI); 4-TBI and cyproheptadine (nonspecific serotonin receptor antagonist; A) (5 mg/kg) given by gastric gavage one hour prior to behavioral tests (TBI+A); 5-TBI followed by 14 days of essitalopram (SSRI) and cypro heptadine (A) given 1 hour prior to behavioral tests (TBI+SSRI+A). Elevated T-maze test and open field test applied to all groups and then blood corticosterone, prefrontal cortex tissue 5-HT and 5-HT 2A receptor quantities measured. Prefrontal cortex neuron density histologically evaluated. Results: In the TBI group, the time spent in the peripheral cells, the time spent in the elevated T-maze closed arms, and serum corticosterone levels found to increase as a result of anxiety. Neuronal density decreased in prefrontal cortex. SSRI treatment reduced the time spent on the closed arms in the elevated T-maze test. SSRI decreased serum corticosterone levels and increased neuronal density. Tissue serotonin levels decreased in all groups exposure to TBI compared to sham group. 5-HT 2A receptor levels were higher in the TBI and A group. Conclusion: SSRIs showed anxiolytic effect for anxiety, secondary to TBI in immature rats. (Anatolian Journal of Psychiatry 2019; 20(4):368-376)

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