Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.)

This study was conducted to evaluate the effect of NaCl and peg-induced osmotic stress on the germination and early seedling growth of wild mustard (Sinapis arvensis L.) in 2016. The seeds of mustard used in this study were obtained from plants growing under wild conditions in the Konya, Turkey. Trials were carried out to study the effects of osmotic potential of polyethylene glycol (PEG-6000) solutions at seven levels (0, -2, -4, -6, -8, -10 and -12 bars) and salt concentrations at eight levels (0, 2, 5, 10, 15, 20, 25, 30 and 35 dSm-1) on seedling growth of wild mustard. Under PEG and salt stress the radicula length (cm), plumula length (cm), seedling fresh weight (mg), seedling dry weight (mg), final germination percentage (%), germination index (%) and seedling vigor index (%) were measured in the study. Final germination percentage and seedling growth parameters decreased by concentration of -4 to -10 bar for PEG stress; final germination percentage decreased by 10 dSm-1 (-4.4 bar) and seedling growth parameters decreased by 5 dSm-1 (-2.2 bar) for NaCl stress. Both NaCl and PEG treatments decreased final germination percentage and seedling growth properties in wild mustard, but the effects of NaCl compared to PEG were less on germination. Seed germination was completely inhibited at -12 bar for PEG stress, 35 dSm1 (-15.6 bar) for NaCl stress.

NaCl ve PEG’e Bağlı Osmotik Stresin Yabani Hardal (Sinapis arvensis L.)’ın Çimlenme ve Fide Gelişimi Özellikleri Üzerine Etkisi

Bu çalışma farklı NaCl ve PEG uygulamalarının yabani hardalın (Sinapis arvensis L.) çimlenme ve erken vejetatif gelişme üzerine etkisini belirlemek amacıyla 2016 yılında yürütüldü. Çalışmada kullanılan yabani hardal tohumları Konya’da yabani koşullarda yetişen bitkilerden alınmıştır. Denemede yedi farklı polietilen glikol (PEG-6000) solüsyonu (0, -2, -4, -6, -8, - 10 ve -12 bar) ve dokuz farklı NaCI solüsyonu (0, 2, 5, 10, 15, 20, 25, 30 ve 35 dSm-1) kullanılmıştır. Farklı PEG ve NaCl uygulamalarında kök uzunluğu (cm), sürgün uzunluğu (cm), fide yaş ağırlığı (mg), fide kuru ağırlığı (mg), final çimlenme oranı (%), çimlenme indeksi (%) ve fide canlılık indeksi ölçümleri yapılmıştır. Çalışma sonucunda, final çimlenme oranının ve fide büyüme parametrelerinin artan PEG konsantrasyonlarına (-4 / -10 bar) bağlı olarak azaldığı; final çimlenme oranının 10 dSm-1 (-4,4 bar)’den itibaren azalmaya başladığı ve fide büyüme parametrelerinin 5 dSm-1 (-2,2 bar)’den itibaren azalmaya başladığı belirlenmiştir. Ayrıca, NaCl ve PEG uygulamalarının çimlenme ve fide gelişimini düşürdüğü; fakat PEG’e kıyasla NaCl’ün etkisinin çimlenmede daha az olduğu; çimlenmenin -12 bar PEG ve 35 dSm-1 (-15,6 bar) NaCl dozlarında tamamen durduğu tespit edilmiştir.


Werner, J. E., and R. R. Finkelstein. 1995. Abidopsismutants with reducedc response to NaCl and osmotic stress. Physiologia Plantarum 93: 659-666.

Warwick, S. I., and L. D. Black. 1991. Molecular systematics of Brassica and allied genera (Subtribe Brassicinae Brassicae)-chloroplast genome and cytodeme congruence. Theor. Appl. Genet. 82: 81-92.

Toosi, A. F., B. B. Bakar, and M. Azizi. 2014. Effect of Drought Stress by Using PEG 6000 on Germination and Early Seedling Growth of Brassica juncea Var. Ensabi, Scientific Papers. Series A. Agronomi Vol: 52: 360-363.

Tilaki, G. A. D., B. Behtari, and B. Behtari. 2009. Effect of Salt and Water Stress on the Germination of Alfaalfa (Medicago Sativa L.) Seed 2: 158-164.

Temizel, K. E., M. S. Odabas, N. Senyer, G. Kayhan, S. Bajwa, O. Caliskan, and E. Ergun. 2014. Comparision of some models for estimation of reflectance of hypericum leaves under stress conditions. Central European Journal of Biology 9 (12): 1226-1234.

Taherkhani, T., N. Rahmani, and A. Pazoki. 2013. The effect of Hydro-priming on Germination of Mustard Seeds under Draught Stress Conditions. Life Science Journal, Vol:10, 3pages. http://www.lifesciencesite. com/lsj/life1003s/058_16381life1003s_392_395.pdf.

Srivastava, S., B. Fristensky, and N. N. V Kav. 2004. Constitutive expression pf Pr10 protein enhances the germination of Brassica napus under saline condition. Plant Cell Physiol. 45: 1320-1324.

Singh, M. 2011. Varietal differences of salinity tolerance at germination stage in mustard genotypes, Prog. Agric. 11 (2): 468-471.

Saeedipour, S. 2009. Appraisal of Some Physiological Selection Criteria for Evaluation of Salt Tolerance in Canola (Brassica napus L.). International Journal of Applied Agricultural Research 4 (2): 179-192.

Rollins, R. C. 1981. Weeds of the Cruciferae (Brassicaceae) in North America. J. Arnold. Arbor. 62: 517-540.

Prakash, S., Y. Takahata, P. B. Kirti, and V. L. Chopra. 1999. Biology of Brassica Coenospecies. pp. 59-106. In: Gomez-Campo C., (ed.) Cytogenetics. Elsevier Science, Amsterdam.

Okcu, G., M. D. Kaya, and M. Atak. 2005. Effects of Salt and Drought Stresses on Germination and Seedling Growth of Pea (Pisum sativum L.). Turk J Agric For. 29: 237-242.

Odabas, M. S., K. E. Temizel, O. Caliskan, N. Senyer, G. Kayhan, and E. Ergun. 2014. Determination of reflectance values of hypericum’s leaves under stress conditions using adaptive network based fuzzy inference system. Neural Network World 24 (1): 79- 87.

Murillo-Amador, B., R. Lopez-Aguilar, C. Kaya, J. LarrinagaMayoral, and H. A. Flores. 2002. Comparative effect of NaCl and PEG on germination emergence and seedling growth of cowpea. Journal of Agronomy and Crop Sciences 188: 235-47.

Mohammadkhani, N., and R. Heidari. 2008. Effects of Drought Stress on Soluble Proteins in two Maize Varities. Turk J. Biol. 32: 23-30.

Macar, K. T. 2008. Effects of Water Deficit Induced by PEG and NaCl on Chickpea (Cicer arietinum L.) Cultivars and Lines at Early Seedling Stages. G. U. Journal of Science 22 (1): 5-14.

Luan, Z., M. Xiao, D. Zhou, H. Zhang, Y. Tian, Y. Wu, B. Guan, and Y. Song. 2014. Effects of Salinity, Temperature, and Polyethylene Glycol on the Seed Germination of Sunflower (Helianthus annuus L.). Hindawi Publishing Corporation Scientific World Journal, Article ID 170418. Vol: 2014, 9 pages. http://dx.doi.org/10.1155/2014/170418.

Khan, M. A., and S. Gulzar. 2003. Germination responses of Sporobolus ioclados: A saline desert grass. J. Arid Environ. 55: 453-464.

Khajeh-Hosseini, M., A. A., Powell, and I. J. Bingham. 2003. The interaction between salinity stress and seed vigor during germination of soybean seeds. Seed Sci. Technol. 31: 715-725.

Kayacetin, F., H. Ogut, H. Oguz, I. Subasi, and H. Deveci. 2016. Determination of the Effect of Row Spacing, and Fall and Spring Sowing on Composition of Fatty Acid and Biodiesel Fuel Characteristics of Mustard (Sinapis arvensis L.). Ciência e Técnica Vitivinícola Journal (ISSN: 0254-0223) 21 (11): 54-69.

Kandil, A.A., A.E. Sharief, W.A.E. Abido, and M.M.O. Ibrahim. 2012. Response of some canola cultivars (Brassica napus L.) to salinity stress and its effect on germination and seedling properties. J. Crop Sci. 3:95- 103.

Jamil, M., D. B., Lee, K. Y., Yung, M. Ashraf, S. C. Lee, and E. S. Rha. 2006. Effect of salt (NaCl) stress on germination and early seedling growth of four vegetables species. Journal of Central European Agriculture 7 (2): 273-281.

Huang, J., and R. E. Redmann. 1995. Salt Tolerance of Hordeum and Brassica Species During Germination and Early Seedling Growth. Can. J. Plant Sci. 75: 815- 819.

Heshmat, O., H. A. Saeed, and K. Fardin. 2011. The improvememt of seed germination traits in canola (Brassica napus L.) as affected by saline and drought stress Journal of Agricultural Technology 7 (3): 611- 622.

Gulzar, S., and M. A. Khan. 2001. Germination of a holophytic grass Aehtopus lagopoides. J. Ann. Bot. 87: 3119- 3329.

Greenway, H., and R. Munns. 1980. Mechanisms of Salt Tolerance in Nonhalophytes. Annu. Rev. Plant Phy. 31: 149-190.

Francois, L. E. 1996. Salinity effects on four sunflower hybrids. Agronomy Journal 88 (2): 215-219.

Effect of NaCl on Germination and Early Seedling Growth of Brassica juncea var. Ensabi. International Journal of Agronomy and Plant Production 4 (11): 3004-3011.

Eryilmaz, T., and H. Ogut. 2011. The effect of the different mustard oil biodiesel blending ratios on diesel engines performance. Energy Education Science and Technology Part A-Energy Science and Research 28(1):169-180. Fallah-Toosi, A., and B. B. Baki. 2013.

Böhm, W. 1979. Methods of Studying Root Systems, Springer-Verlag, Berlin. Bybordi, A., and J. Tabatabaei. 2009. Effect of salinity stress on germination and seedling properties in Canola cultivars (Brassica napus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37 (1): 71-76.

Blackshaw, R., E. Johnson, Y. Gan, W. May, D. McAndrew, V. Barthet, T. McDonald, and D. Wispinski. 2011. Alternative oilseed crops for biodiesel feedstock on the Canadian prairies. Canadian Journal of Plant Sci. 9 (5): 889-896.

Bing, D. J., R. K. Downey, and G. F. W. Rakow. 1996. Assessment of transgene escape from Brassica rapa (B. Campestris L.) into Brassica nigra of Sinapis arvensis. Plant Breeding 115: 1-4.

Baranova, E. N., and A. A. Gulevich, and V. Y. Polyakov. 2006. Effects of NaCl, Na2SO4, and mannitol on storage lipid mobilization in the cotyledons and roots of purple alfalfa seedlings. Russian Journal of Plant Physiology 53: 779-784.

Ayaz, F. A., A. Kadıoğlu, and R. T. Utgut. 2000. Water stress effects on the content of low molecular weight carbohydrates and phenolic acids in cienanthe serosa. Canadian J. Plant Sci. 80: 373-378.

Asmare, H. A. 2013. Impact of salinity on tolerance, vigor, and seedling relative water content of haricot bean (Phaseolus vulgaris L.) cultivars. Journal of Plant Sci. 1 (3): 22-27.

Ashraf, M., and T. McNeilly. 2004. Salinity tolerance in Brassica oilseeds. Crit. Rev. Plant Sci. 23: 157-174.

Anonymous. 1996. International Rules for Seed Testing, Rules (ISTA) 1996. Seed Sci. Technol. 24 Suppl.

Kaynak Göster

143 56

Sayıdaki Diğer Makaleler

The Effects of Different Doses of Organic Chicken Fertilizer on the Element Analysis of Sweet Basil (Ocimum basilicum L.)


Determination of Some Characteristics of Perennial Ryegrass (Lolium perenne L.) Populations Collected from Natural Areas of Eskisehir for Breeding Purposes

İlker ERDOĞDU, A. Levent SEVER, Celalettin AYGÜN, METİN TUNA

Wild Plants Used as Herbal Tea in Antakya and Defne Provinces of Hatay


Türlerarası Melezleme Yoluyla Lahana Kök Uru (Plasmodiophora brassicae Woronin)'na Karşı Dayanıklı Hatların Geliştirilmesinde Embriyo Kültür Tekniğinin Kullanım İmkanı


Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.)


Determination of Some Characteristics of Cocksfoot (Dactylis glomerata L.) Populations Collected from Natural Areas of Eskisehir for Breeding Purposes

İlker ERDOĞDU, A. Levent SEVER, Celalettin AYGÜN, METİN TUNA

Patates Böceği [Leptinotarsa decemlineata Say.(Coleoptera: Chrysomelidae)]’nin Nevşehir İlinde Yaşamsal Etkileşim ve Çeşitliliği Üzerine Bir Ön Çalışma


Identification and Evaluation of Propagation Techniques of Dianthus orientalis Adams.


Preliminary Study on Edible Insect Species Cybister limbatus (Fabricius 1775) and Its Heavy Element Contents


Intra-Genetic Variation within Olive Cultivar 'Nabali' in Palestine by Microsatellite and Random Amplified Polymorphic DNA