Duran ŞİMŞEK, HASAN PINAR, Nedim MUTLU

10327

Moleküler ıslah yöntemleri kullanılarak tospovirus ve tobamoviruslere dayanıklı yeni dolmalık biber ( capsicum annum l.) hat ve çeşitlerinin geliştirilmesi

Biber yetiştiriciliğinde biber hafif benek virüsü (PMMoV), tütün mozaik virüs ü (TMV) ve Domates Lekeli Solgunluk Virüsü (TSWV) ana sınırlayıcı faktörlerdendir. Günümüzde üretim sahası ve zamanına bağlı olarak en az iki ve ya daha fazla hastalık ve de zararlı etmen e karşı dayanıklılığ ı ihtiyaç duyulmaktadır. Bu çalışmada PMMoV, TMV ve TSWV gibi bazı hastalıklara dayanıklılığa bağlı olarak geliştirilmiş L3, L4 ve Tsw alleleri ile ilişkili moleküler markırlar yardımıyla söz konusu virüslere dayanıklı biber hat ve çeşitlerinin geliştirilmesi amaçlanmıştır. Bu amaç için markır yardımlı g eriye melezleme ve/veya kendileme ile elde edilen 3890 adet dolmalık biber bitkisi söz konusu markırlarla testlenmiş ve hem dayanıklı hem de agronomik olarak üstün olan 72 adet hat ebeveyn olarak belirlenmiştir. Bu hatlar içerisinden her 3 dayanıklılık allelini taşıyacak şekilde 78 adet melez yapılmış ve bu melezler arasından 1 tanesi aday çeşit olarak belirlenmiştir. Bu çalışma, moleküler ve klasik ıslah yöntemleri birlikte kullanılarak biberde PMMoV, TMV ve TSWV gibi hastalıklara dayanımlı çeşit geliştirilebileceğini göstermektedir.

Development of new bell pepper (capsicum annum l.) lines and cultivars resistance to tospovirus and tobamovirus u sing molecular breeding methods

Pepper mild mottle virus (PMMoV), Tobacco Mosaic Virus (TMV) and Tomato Spotted Wild Virus (TSWV) are main limiting factor in pepper production. Nowadays, it is needed to at least two or more resistance to disease agents according as production area and season. In this study, it was aimed to develop PMMoV, TMV and TSWV resistance pepper line and cultivars using molecular markers which developed as linked to L3 , L4 and Tsw allele s which provide resistance to these virus. For this aim, 3890 bell pepper lines which developed using backcrossing or selfing was tested and determined 72 lines as parents which resistant and were also sup erior with regard to agronomic traits . Seventy eighty crosses which consist three resistance alleles together in one hybrid genotype were done between 72 pepper lines and one of cross es was determined as candidate cultivar. This study showed that it can b e developed resistance cultivars to viruses such as PMMoV, TMV and TSWV using molecular and classic breeding methods together in pepper breeding program.

___

  • Ben Chaim, A., Grube, R.C., Lapidot, M., Jahn, M., Paran, I . , 2001. Identification of quantitative trait loci associated with resistance to cucumber mosaic virus in Capsicum annuum.Theor. Appl. Genet. 102, 1213 −1220.
  • Boukema, I.W., 1980. Allelism of gene controlling resistance to TMV in Capsicum L. E uphytica 29, 433 −439.
  • Boiteux, L.S. 1995. Allelic relationships between genes for resistance to tomato spotted wilt tospovirus in Capsicum chinense. Theor. Appl. Genet. 90:146- 149.
  • Caranta, C., Thabuis, A., Palloix, A., 1999. Development of a CAPS marker for the Pvr4 locus: a tool for pyramiding potyvirus resistance genes in pepper. Genome 42, 1111 −1116.
  • Cho, J.J., Mau, R.F.L., Gonsalves, D., Mitchell, W.C., 1986. Reservoir weed hosts of tomato spotted wilt virus. 70(11): 1014- 1017.
  • Choi, G.S., Kim, J.H., Kim, J.S., Kim, H.R., 2004. Tobamo viruses of gren peppers growing on hydroponic systems.Res. PlantDis. 10, 194 −197.
  • Çelik, N., Özalp, R., Göçmen, M., 2012. Antalya ilinde örtüaltı biber yetiştiriciliğinde Patates Y virüsü (PVY) patotiplerinin belirlenmesi ve bazı biber çeşitlerinin PVY’ye karşı reaksiyonları Bitki Koruma Bülteni 2012, 52(3):235- 246 .
  • Doyle, J.J., Doyle J.L., 1990. Isolation of plant DNA fromfreshtissue. Focus 12: 13 - 15 .
  • Ekbiç E., Abak K., Yılmaz, M.A,. 1997. A New PVY Pathotype on Pepper Along Mediterranean CoastalArea of Turkey. Proc.10th Cong, Medit. Phytopath. Union, Montpellier, 1 - 5 June 1997. 187- 189.
  • Ekbiç E., Abak K. , Yılmaz M.A. , 1997. A New PVY Pathotype on Pepper Along Mediterranean Coastal Area of Turkey . Proc.10th Cong, Medit. Phytopath. Union, Montpellier, 1 -5 June 1997. 187 -189.
  • German, T.L., Ullman, D.E. , Moyer, J.W. , 1992. Tospoviruses: diagnosis, molecular biology, phylogeny and vector relationships. Annu. Rev. Phytopathol., 30: 315 -348.
  • Goldbach, R., Peters, D. , 1994. Possible causes of the emergence of tospovirus diseases. Semin. Virol. 5: 113–120.
  • Griep, R.A., Prins, M., vanTwisk, C., Keller, H.J., Kerschbaumer, R. J., Kormelink, R., 2000. Application of phagedisplay in selecting Tomato spotted wilt virus - specificsingle- chain antibodies (scFvs) for sensitive diagnosis in ELISA. Phytopathology, 90, 183- 190 .
  • Han, J.H., Lee, C.H., La, Y.J., 2001a. Pathotype of Tobamo virus isolates from commercial red pepper seeds. Kor. J.Hort. Sci. And Technol. 19, 309−453.
  • Han, J.H., Sohn, S.H., La, Y.J. , 2001b. Identification and characterization of Tobamo viruses isolated from commercial pepper seeds. Res. PlantDis. 7, 164− 169.
  • Ikegashira, Y., Ohki, T., Ichiki, U.T., Higashi, T., Hagiwara, K., Omura, T., Honda, Y., Tsuda, S. , 2004. An immunological system for the detection of Pepper mild mottle virus in soil from green pepper fields. Plant Dis. 88, 650− 656.
  • Kim, H.J., Han, J - H, Yoo, J.H., Cho, H.J., Kim, B. , 2008. Development of a Sequence Characteristic Amplified Region Marker linked to the L4 Locus Conferring Broad Spectrum Resistance to Tobamo viruses in Pepper Plants Mol. Cells, Vol. 25, No.2, pp. 205 -210.
  • Matsunaga, H., Saito, T., Hirai, M., Nunome, T., Yoshida, T., 2003. DNA markers linked to pepper mild mottle virus (PMMoV) resistant locus (L4) in Capsicum. J. Jpn. Soc. Hort. Sci. 72, 218 −220.
  • Moury, B., Palloix, A., Selassie, K.G., Marchoux, G. , 1997. Hypersensitive resistance to tomato spotted wilt virus in three Capsicum chinense accessions is controlled by a single gene and is overcome by virulent strains. Euphytica 94:45- 52.
  • Moury, B., Pflieger, S., Blattes, A., Lefebvre, V. Polloix. A., 2000. A CAPS marker to asist selection to tomato spotted wilt virus (TSWV) resistance in pepper. Genome43:1 37- 142.
  • Parrella, G., Gognalons, P. , Gebre- Selassiè, K. , Vovlas , C. , Marchoux, G. , 2003. An update of the host range of Tomato spotted wilt virus . J. Plant Pathol., 85: 227 -264.
  • Pares, R.D., Gunn, L.V., 1989. The role of non- vectored soil transmission as a primary source of infection by pepper mild mottle and cucumber mosaic viruses in glasshouse grown capsicum in Australia. J. Phytopathol. 126, 353 −360.
  • Rosellό, S., Diéz, M.J., Nuez, F., 1996. Viral diseases causing the greates economic losses to the tomato crop. I. The tomato spotted wilt virus - a review. Sci. Hort., 67:117- 150.
  • Sugita, T . , Yamaguchi, K. , Sugimura, Y. , Nagata, R, Yuji , K, Kinoshita, T, Todoroki , A. 2004. Development of SCAR marker linked to L3 gene in Capsicum . Breed Sci 54:111 – 115.
  • Şevik, M. A., 2011 . Occurrence of pepper mild mottle virus in greenhouse grown pepper ( Capsicum annuum L.) in the West Mediterranean region of Turkey African Journal of Biotechnology Vol. 10(25), pp. 4976- 4979, 8 June, 2011 .
  • Tomita, R ., Murai, J ., Miura, Y., Ishihara, H., Liu S., Kubotera, Y., Honda A., Hatta R ., Kuroda, T ., Hamada, H., Sakamoto, M ., Munemura I., Nunomura, O., Ishikawa, K., Genda Y., Kawasak,i S., Suzuki K., Meksem, K., Kobayashi, K., 2008. Fine mapping and DNA fiber FISH analysis locates the tobamo virus resistance gene L3 of Capsicum chinense in a 400- kb region of R- like genes cluster embedded in highly repetitive sequences. Theor Appl Genet. 2008 Nov;117(7):1107 -18. doi: 10.1007/s00122- 008- 0848- 6. Epub 2008 Jul 29.
  • Toyoda, K., Hikichi, Y., Takeuchi, S., Kuroda, T., Okumura, A., Nasu, Y., Okuno, T., Suzuki, K., 2004. Epidemiological aspects of the japanese tobamo virus strain, pepper mild mottle virus (PMMoV) infecting the L2 resis tance genotype of gren pepper ( Capsicum annuum L.). Scientific Reports of the Faculty of Agriculture Okayama University. 93,19− 27.
  • Uhrig, J.F., T.R. Soellick, C.J. Minke, C. Philipp, J.W. Kellman and J.W. Schreier, 1999. Homotypic interaction and multimerization of nucleocapsid protein of Tomato spotted wilt tospovirus: identification and characterization of two interacting domains. Proc. Natl. Acad. Sci. USA., 96: 55- 60.
  • Yang, H. B. , Liu, W. Y., Kang, W. H. , Jahn, M. , Kang, B.C. , 2009. Development of SNP markers linked to the L locus in Capsicum spp. by a comparative genetic analysis Molecular Breeding . 24 ( 4): 433- 446.
Alatarım-Cover
  • ISSN: 1304-2653
  • Başlangıç: 2015
  • Yayıncı: Alata Bahçe Kültürleri Araştırma Enstitüsü
Arşiv
Sayıdaki Diğer Makaleler

Killi toprak koşullarında yetiştirilen kütdiken limon ç eşidinde n, p ve k b esin elementlerinin mevsimsel dağılımı

Sefa POLATÖZ, TURGUT YEŞİLOĞLU

Moleküler ıslah yöntemleri kullanılarak tospovirus ve tobamoviruslere dayanıklı yeni dolmalık biber ( capsicum annum l.) hat ve çeşitlerinin geliştirilmesi

Duran ŞİMŞEK, HASAN PINAR, Nedim MUTLU

Kayseri ilinde yetişen ceviz ( juglans regia l.) genotiplerinde fenolojik özelliklerin ve yan dal verimlerinin belirlenmesi

Kadir PARİS, AYDIN UZUN

Bazı Sofralık kayısı genotiplerinin çiçek tozu canlılık ve çimlenme düzeylerinin belirlenmesi

Mustafa BİRCAN, HASAN PINAR, MUSTAFA ÜNLÜ

Sergen koşullarda aşılı crimson tide çeşidi karpuzlarda kalite parametrelerindeki değişimler

Veysel ARAS, AHMET ERHAN ÖZDEMİR, HALİT YETİŞİR, ELİF ÇANDIR, ZEHRA GÜLER, Ömer ASLAN, Durmuş ÜSTÜN, Özay BALTAER, MUSTAFA ÜNLÜ

Evaluating mycorrhizal dependency of some p epper genotypes during seedling stage

HASAN PINAR, İBRAHİM ORTAŞ, Davut KELEŞ