Anthurium sp.'de Tomato Spoted Wilt Virus (TSWV) Enfeksiyonu ve Moleküler Karakterizasyonu

Çalışma Antalya/Türkiye'de yetiştirilen Anthurium sp,'de (Anthurium scherzerianum) bitkilerinde virüs benzeri simptomlar görülmesi üzerine 2015 yılında gerçekleştirilmiştir. Gözlemler sırasında Flamingo çiçeği bitkilerinin yapraklarında, sistemik klorotik mozaik ve düzensiz sarımsı koyu kahverengi nekrotik lekeler gözlenmiştir. Tanılanma için yaprak DAS-ELISA tekniğine göre AMV (Alfalfa Mosaic Virus), ArMV (Arabis Mosaic Virus), CMV (Cucumber Mosaic Virus), Poty (Group Poty Virus), SLRSV (Strawberry Latent Ringspot Virus), TMV (Tobacco Mosaic Virus), TNV (Tobacco Necrosis Virus), ToMV (Tomato Mosaic Virus), ToRSV (Tomato Ringspot Virus), Tospogrup (Tospovirus), TRSV (Tobacco Ringspot Virus), TSWV (Tomato Spotted Wilt Virus), Impatiens necrotic spot virus (INSV),PVX (Potato Virus X), SqMV (Squash mosaic virus) ve ZYMV (Zucchini yellow mosaic virus) gibi süs bitkilerinde bulunma olasılığı bulunan virüsler için testlenmiştir. DAS-ELISA sonuçları pozitif örneklerden ekstrakte edilen toplam RNA'ların RTPCR'da kullanılmasıyla doğrulanmıştır. RT-PCR, sense TSWV L1F ve antisense primerlerine TSWV L2R göre düzenlenmiştir. RT-PCR sonucu kılıf proteinine ait 276 bp cDNA bantları %2'lik agaroz jelde gözlenmiştir. RT- PCR ürünlerinin direkt sekanslaması ve yapılan BLAST sonucunda biber TSWV izolatları ile %96-99 oranında benzerlik gösterdiği tespit edilmiştir. Anthurium bitkisinde TSWV enfeksiyonu serolojik ve moleküler olarak saptanmıştır. Bu çalışma yalnızca TSWV'nin Anthurium bitkisindeki Türkiye için ilk kaydı olup aynı zamanda etmenin türler arasında kontrolsüz yayılımının kanıtıdır.

Infection and Molecular Characterization of Tomato Spotted Wilt Virus (TSWV) on Anthurium sp.

This research wasconducted on Anthurium sp (Anthurium scherzerianum)observed the virus-like symptoms which was grown in Antalya in Turkey in 2015. During the observations, systemic mosaic and irregular yellowish dark brown necrotic spots were recorded on Flamingo flower plants. To identification, leaf samples were tested by DAS-ELISA using a specific polyclonal antiserum AMV (Alfalfa Mosaic Virus), ArMV (Arabis Mosaic Virus), CMV (Cucumber Mosaic Virus), Poty (Group Poty Virus), SLRSV (Strawberry Latent Ringspot Virus), TMV (Tobacco Mosaic Virus), TNV (Tobacco Necrosis Virus), ToMV (Tomato Mosaic Virus), ToRSV (Tomato Ringspot Virus), Tospo (Tospovirus), TRSV (Tobacco Ringspot Virus), TSWV (Tomato Spotted Wilt Virus), Impatiens necrotic spot virus (INSV), PVX (Potato Virus X), SqMV (Squash Mosaic Virus) and ZYMV (Zucchini Yellow Mosaic Virus) presence and identification of common ornamental plant viruses. Then samples which were positive in DAS-ELISA were confirmed by RT-PCR using total RNA extracted from leaves RT-PCR assay was conducted by primer pairs Sense TSWV L1r and Antisense TSWVL2f. As a result of RT-PCR, 276 bp cDNA bands were observed in 2% agarose gel. RT-PCR products were sequenced directly, and as the result of BLAST using datas from sequences, TSWV isolate in Anthurium showed 96-99 % similarity with different pepper isolates of TSWV. In Turkey, the occurrence of TSWV has reported on Anthurium sp. by serologically and molecular techniques. This study is not only the first record of TSWV in Turkey on Anthurium sp but also is evidence of uncontrolled distribution between plant species.

PDF

___

Adkins, S., Zitter, T., Momol, T., 2005. Tospoviruses (Family Bunyaviridae, Genus Tospovirus). Fact Sheet PP- 212, One of a Series of The Plant Patology Department,
Florida Cooparative Extension Services Institute of Food And Agricultural Sciences, University of Florida.
Almeida Img; Malavolta Junior Va; Destéfano, Sal; Paradela Filho O; Balanı Dm; Ferreira M; Santos Db., 2002. Mancha foliar em antúrio causada por Acidovorax anthurii: primeira constatação no Brasil. Summa Phytopathologica 28: 96.
Arli-Sökmen, M., Mennan, H., Sevik, M.A., Ecevit, O. 2005. Occurence of viruses in fieldgrown pepper crops and some of their reservoir weed hosts in Samsun, Turkey. Phytoparasitica 33: 347-358
Azeri, T., 1981. Preliminary report of tomato spotted wilt virus and its epidemiy on tobacco in the Çanakkale region of Türkiye. J. Turkish Phytopath., 10 (2-3): 79-87.
Azeri, T., 1994. Detection of tomato spotted wilt virus in tobacco and tomato cultivars by enzyme linked immunosorbent assay. J. Turkish Phytopath., 23 (1): 37-46.
Chen, J., McConnell, D.B., Henny, R.J., Everitt, K.C., 2003. Cultural Guidelines for Commercial Production of Interiorscape Anthurium. ENH956. Gainesville: University. Cho, J.J., Mitchel, W.C., Mau, R.F.L., Sakımura, K., 1987. Epidemiology of Tomato Spotted Wilt Virüs Disease on Crisphead Lettuce inHawaii. Plant Disease, 71: 505- 508.
Clark M.F., Adams, A.N., 1977. Characteristics Of The Micro-Plate Method Of Enzime Linked Immuno Sorbent Assay For The Detection Of Plant Viruses. Journal Of General Virology. 34, 475-483.
Eckel, C.S., Cho, K., Walgenbach, J.F., Kennedy, G.G., d Moyer, J.W., 1996. Variation in Thrips Species Composition in Field Crops and İmplications for Tomato Spotted Wilt Epidemiology in North Carolina.Entomol.exp., 78: 19- 29.
German, T.L., Ullman, D.E. Moyer, J.W., 1992. Tospovirüses: Diagnosis, Molecular Biology, Phylogeny and Vector Relationships. Ann. Rev. Phytopathol. 30: 315- 348.
Goldbackh, R., Peters, D., 1994. Possible Causes of the Emergence of Tospovirüs Diseases. Seminers in Virology, 3: 113- 120.
Güldür, M.E., 1997., Şanlıurfa ili için yeni bir virüs: Domates lekeli solgunluk virüsü (Tomato spotted wilt virus). HR Ü. Ziraat Fakültesi Dergisi. 1 (3): 71-76.
Güldür, M.E., Marchoux, G., Yurtmen, M., Yılmaz, M.A., 1995. Mersin ve çevresinde yetiştirilen domateslerde zararlı yeni bir virüs: Tomato spotted wilt virus. VII: Türkiye Fitopatoloji Kongresi, 26/29 Eylül 1995, Adana, s: 303-305.
Lacerda, J.P., 2006. Doenças fúngicas e bacterianas em algumas espécies de Araceae. Lavras: UFLA. 81p (Dissertação mestrado).
Şevik, M.A., 2011 Domates lekeli solgunluk virüsü (TSWV)'nün Tarımsal Ürünlerde Meydana Getirdiği Ekonomik Kayıplar HR.Ü.Z.F. Dergisi, 2011, 15(1).
Şevik, M.A., 2015 Sebze Üretimini Tehdit Eden Viral Hastalık Etmeni: Domates lekeli solgunluk virüsü (Tomato spotted wilt virus - TSWV) Iğdır Üni. Fen Bilimleri Enst. Dergisi Iğdır Univ. J. Inst. Sci. & Tech. 5(2): 17-23, 2015.
Miura N.S.; Beriam LO.S., Rivas E.B., 2013. Detection of Cucumber mosaic virus in commercial anthurium crops and genotypes evaluation. Horticultura Brasileira 31: 322- 327.
Norman D.J., Ali, G., 2012. Anthurium Diseases: Identification and Control in Commercial Greenhouse Operations UF/IFAS Extension. Original publication date February 2012.
Reviewed March 2015. Visit the EDIS website at http://edis.ifas.ufl.edu. Rivas, E.B.; Duarte L.M.L; Alexandre M.A.V., Galleti S.R., 1997. Dasheen mosaic virus in Anthurium species. Virus Reviews & Research 2: 192-193.
Robbs, C.F., 1953. A mancha bacteriana das aráceas. Agricultura e Pecuária, Rio de Janeiro 24: 18-19.Tomato Spotted Wilt Virüs in Common Agapanthus. Plant Disease, 84: 491.
Soler, S., Diez, M.J., Nuez, F., 1998. Effect of temperature regime and growth stage interaction on pattern of virus presence inTSWV-resistant accessions of Capsicum chinense. Plant Disease, 82: 1199-1204.
Uchida., J.Y., Ogata, D., Nagata, N., 1999. Tomato Spotted Wilt Virus on Anthurium Plant Disease June 1999 PD-17
Ullman, D.E., Cho, J.J., Mau, R.F.L., Westcot, D.M., Custer, D.M., 1992. A midgut barrier to Tomato Spotted Wilt Virüs acguistion by adult western flower thrips. Phytopathology, 82: 1333- 1342
Werkman, A.W. (Arjen) J.Th.J. (Ko) Verhoeven, J.W. (Annelien) Roenhorst 1989 Plant species found infected by Tomato spotted wilt virus and Impatiens necrotic spot virus at the Dutch Plant Protection Service since 1989.
Wilson, C.R., Wilson, A.J, Petehybridge, S.J., 2000. First record of tomato spotted wilt virus in common agapanthus. Plant Disease 84: 491.