Objective: The aim of this study was to compare serum and synovial fluid levels of matrix metalloproteinase-13 (MMP-13) and tumor necrosis factor-alpha (TNF-?) in 2 stages of osteoarthritis, and investigate their correlation with Western Ontario and McMaster Universities Arthritis Index (WOMAC) scores.Methods: Forty-two patients (mean age: 64±8.8 years) with grade 3 and grade 4 knee osteoarthritis according to Kellgren-Lawrence criteria were enrolled in the study and underwent total knee arthroplasty. TNF-? and MMP-13 levels were measured preoperatively from venous blood samples and intraoperatively from knee synovial fluid via enzyme-linked immunosorbent assay. Preoperative and 1-month postoperative knee functions were assessed by WOMAC.Results: Grade 4 synovial fluid MMP-13 (4.76±5.82 pg/ml) was elevated compared to grade 3 (3.95±4.45), whereas grade 3 serum MMP-13 (1.128±0.308 pg/ml) was found elevated compared to grade 4 (1.038±0.204) (p=0.438, p=0.430, respectively). Grade 4 serum TNF-? (0.253±0.277) was elevated compared to grade 3 (0.206±0.219), whereas grade 3 synovial fluid TNF-? (0.129±0.052) was elevated compared to grade 4 (0.118±0.014). Positive correlation was observed between synovial fluid MMP-13 levels and postoperative WOMAC scores. Mean serum TNF-? (0.226±0.246 pg/ml) was elevated compared to synovial levels (0.124±1.59), and synovial MMP-13 (4.31±1.24) was elevated compared to serum levels (1.089±1.519).Conclusion: Despite the systemic increase in TNF-? levels concordant with osteoarthritis grade, MMP-13 levels are elevated via local manner, with a significant correlation with WOMAC scores.
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Atik OS. Is subchondral bone the crucial point for the pathogenesis and the treatment of osteoarthritis? Eklem Hastalik Cerrahisi 2014;25:1.
Martel-Pelletier J, Pelletier JP. Is osteoarthritis a disease involving only cartilage or other articular tissues? Eklem Hastalik Cerrahisi 2010;21:2-14.
Cooper C, Snow S, McAlindon TE, Kellingray S, Stuart B, Coggon D, et al. Risk factors for the incidence and progres- sion of radiographic knee osteoarthritis. Arthritis Rheum 2000;43:995-1000.
Atik OŞ, Tokgöz N. Do periarticular dense bone islands cause cartilage destruction? Eklem Hastalik Cerrahisi 2013;24:39-40.
Tashman S, Collon D, Anderson K, Kolowich P, Anderst W. Abnormal rotational knee motion during running af- ter anterior cruciate ligament reconstruction. Am J Sports Med 2004;32:975-83.
Nishimura A, Hasegawa M, Kato K, Yamada T, Uchida A, Sudo A. Risk factors for the incidence and progression of radiographic osteoarthritis of the knee among Japanese. Int Orthop 2011;35:839-43.
Malemud CJ, Islam N, Haqqi TM. Pathophysiological mechanisms in osteoarthritis lead to novel therapeutic strategies. Cells Tissues Organs 2003;174:34-48.
Miller RE, Miller RJ, Malfait AM. Osteoarthritis joint pain: the cytokine connection. Cytokine 2014;70:185-93.
MacFarlane RJ, Graham SM, Davies PS, Korres N, Tsouchnica H, Heliotis M, et al. Anti-inflammatory role and immunomodulation of mesenchymal stem cells in sys- temic joint diseases: potential for treatment. Expert Opin Ther Targets 2013;17:243-54.
Santangelo KS, Nuovo GJ, Bertone AL. In vivo reduction or blockade of interleukin-1? in primary osteoarthritis in- fluences expression of mediators implicated in pathogen- esis. Osteoarthritis Cartilage 2012;20:1610-8.
Wang W, Kang W, Tang Q, Yao G, Chen Y, Cheng B, et al. Cilostazol prevents the degradation of collagen type II in human chondrocytes. Biochem Biophys Res Commun 2014;451:352-5.
Zhang X, Zhu Y, Chen X, Zhang Y, Zhang Y, Jia Y, et al. Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes. Int Immu- nopharmacol 2014;21:301-8.
Tang P, Hung M-C, Klostergaard J. Human pro-tumor ne- crosis factor is a homotrimer. Biochemistry 1996;35:8216- 25.
Kriegler M, Perez C, DeFay K, Albert I, Lu SD. A novel form of TNF/cachectin is a cell surface cytotoxic trans- membrane protein: ramifications for the complex physiol- ogy of TNF. Cell 1988;53:45-53.
Olszewski MB, Groot AJ, Dastych J, Knol EF. TNF traf- ficking to human mast cell granules: mature chain-depen- dent endocytosis. J Immunol 2007;178:5701-9.
Alaaeddine N, Hilal G, Baddoura R, Antoniou J, Di Bat- tista JA. CCL20 stimulates proinflammatory mediator synthesis in human fibroblast-like synoviocytes through a MAP kinase-dependent process with transcriptional and posttranscriptional control. J Rheumatol 2011;38:1858- 65.
Alaaeddine N, Di Battista JA, Pelletier JP, Kiansa K, Cloutier JM, Martel-Pelletier J. Differential effects of IL- 8, LIF (pro-inflammatory) and IL-11 (anti-inflammatory) on TNF-alpha-induced PGE(2)release and on signalling pathways in human OA synovial fibroblasts. Cytokine 1999;11:1020-30.
Hermann J, Lipp RW, Dunzinger A, Spreizer C, Schaf- fler G, Kvaternik H, et al. Anti-TNF scintigraphy to as- sess TNF-?-associated joint inflammation in rheuma- toid arthritis and osteoarthritis. Clin Exp Rheumatol 2014;32:614.
Quintana JM, Escobar A, Arostegui I, Bilbao A, Azkarate J, Goenaga JI, et al. Health-related quality of life and ap- propriateness of knee or hip joint replacement. Arch Intern Med 2006;166:220-6.
Loeuille D, Chary-Valckenaere I, Champigneulle J, Rat AC, Toussaint F, Pinzano-Watrin A. Macroscopic and microscopic features of synovial membrane inflammation in the osteoarthritic knee: correlating magnetic resonance imaging findings with disease severity. Arthritis and rheu- matism 2005;52:3492-501.
Wassilew GI, Lehnigk U, Duda GN, Taylor WR, Matziolis G, Dynybil C. The expression of proinflammatory cyto- kines and matrix metalloproteinases in the synovial mem- branes of patients with osteoarthritis compared with trau- matic knee disorders. Arthroscopy 2010;26:1096-104.
Bondeson J, Wainwright SD, Lauder S, Amos N, Hughes CE. The role of synovial macrophages and macrophage- produced cytokines in driving aggrecanases, matrix me- talloproteinases, and other destructive and inflammatory responses in osteoarthritis. Arthritis Res Ther 2006;8:187.
Haack M, Pollmacher T, Mullington JM. Diurnal and sleep-wake dependent variations of soluble TNF- and IL-2 receptors in healthy volunteers. Brain Behav Immun 2004;18:361-7.
Payette C, Blackburn P, Lamarche B, Tremblay A, Bergeron J, Bölümü, İstanbul, TürkiyeLemieux I, et al. Sex differences in postprandial plasma tumor necrosis factor-alpha, interleukin-6, and C-reactive protein concentrations. Metabolism 2009;58:1593-601.
Zhou X. Conceptual and methodological issues relevant to cytokine and inflammatory marker measurements in clinical research. Curr Opin Clin Nutr Metab Care 2010;13:541-7.
Benito MJ, Veale DJ, FitzGerald O, Van den Berg WB, Bresnihan B. Synovial tissue inflammation in early and late osteoarthritis. Annals of the rheumatic diseases 2005;64:1263-7.
Aktas E, Sener E, Gocun PU. Mechanically induced ex- perimental knee osteoarthritis benefits from anti-inflam- matory and immunomodulatory properties of simvastatin via inhibition of matrix metalloproteinase-3. Orthopaedics and Traumatology 2011;12:145-51.
Yang Q, Wu S, Mao X, Wang W, Tai H. Inhibition ef- fect of curcumin on TNF-alpha and MMP-13 expression induced by advanced glycation end products in chondro- cytes. Pharmacology 2013;91:77-85.
Park SJ, Cheon EJ, Lee MH, Kim HA. MicroRNA-127-5p regulates matrix metalloproteinase 13 expression and inter- leukin-1beta-induced catabolic effects in human chondro- cytes. Arthritis and rheumatism 2013;65:3141-52.
Kobayashi M, Squires GR, Mousa A, Tanzer M, Zukor DJ, Anton Bölümü, İstanbul, Türkiyeiou J, et al. Role of interleukin-1 and tumor necrosis factor alpha in matrix degradation of human osteoarthritic cartilage. Arthritis and Rheumatism 2005;52:128-35.
Ryu JH, Lee A, Na JH, Lee S, Ahn HJ, Park JW, et al. Op- timization of matrix metalloproteinase fluorogenic probes for osteoarthritis imaging. Amino Acids 2011;41:1113- 22.
Rutgers M, Saris DB, Dhert WJ, Creemers LB. Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection. Arthritis research & therapy 2010;12:114.