Elektrikle Uyarılmanın ve K+ Depolarizasyonunun Sıçan Striatal Dilimlerinde Asetilkolin ve Kolin Salıverilmesine ve Doku Düzeylerine Etkisi

Amaç: Elektrikle ya da yüksek potasyumla uzun süreli uyarılmanın sıçan striatal beyin dilimlerinde asetilkolin ve kolin metabolizmasına etkilerini belirlemek. Yöntemler: Striatal dilimler dinlenme durumunda, elektrikle uyarılarak ya da yüksek potasyumla depolarize edilerek 120-dakika perfüze edildi. Perfüzatta ve dokudaki asetilkolin ve kolin radioenzimatik yöntemle ölçüldü. Bulgular: Dinlenme koşullarındaki dilimlerden ortama 376±20 pmol/mg protein/120 dakika asetilkolin ve 2375±85 pmol/mg protein/120 dakika kolin salıverildi. Uyarılma ile asetilkolin salıverilmesi 5-6 kat artarak elektrikle uyarılmada 2830±174 pmol/mg protein/120 dakika, potasyumla depolarizasyonda ise, 2360±85 pmol/mg protein/120 dakika düzeyine ulaştı. Kolin salıverilmesi elektrikle uyarımda değişmedi. Potasyumla depolarizasyonda ise, kolin çıkışı ilk 20 dakikalık dönemde %50 artmakla p

Effects of Electrical Stimulation and K+ Depolarization On The Release and Contents of Acetlcholine and Choline In Superfused Slices From Rat Striatum

Effects of electrical stimulation and potassium depolarization on acetylcholine and choline release and synthesis in rat brain striatal slices Objective: The aim of the study was to determine effects of electrical stimulation and potassium depolarization on acetylcholine and choline release and synthesis in rat brain striatal slices Methods: Striatal slices from rat brain were perfused with physiological medium under basal and stimulated conditions, either with electrically or high potassium medium, for 120 min. Acetylcholine and choline contents of the perfusate and tissue were assayed radioenzymatically. Results: Striatal slices released 376±20 pmol/mg protein/120 min acetylcholine and 2375±85 pmol/mg protein/120 min choline into the perfusate at rest. When sliced stimulated by high potassium or electrically, acetylcholine release into the perfusate were increased by 5-6 folds and reached to 2360±85 or 2830±174 pmol/mg protein/120 min, respectively. Choline release into perfusate was not change during electrical stimulation. During high potassium depolarization, choline release increased by 50% at first 20-min period but decreased by 25% during the whole of 120-min period. The tissue contents of acetylcholine, but not choline, decreased slightly by 20%; p

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Acıbadem Üniversitesi Sağlık Bilimleri Dergisi-Cover
  • ISSN: 1309-470X
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2010
  • Yayıncı: ACIBADEM MEHMET ALİ AYDINLAR ÜNİVERSİTESİ
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