Biyoimpedans İğne Probu ile Parçacık Boyut Tespiti

Amaç: Sunulan çalışmanın amacı Biyoimpedans iğne probunun in-vitro doku benzeri ortamında test edilmesidir. Çalışma Planı: Biyoimpedans iğne probu ile biyoimpedans cihazı bağlanmış ve kHz aralığında spektrumlar kaydedilmiştir. Prop 18Gauge 1.2x89 mm lomper ponksiyon iğnesi kullanılarak dizayn edilmiştir. Prob 1, 2 ve 4 mikrometre boyutundaki polistiren mikroküreciklerde kullanılarak impedans bilgisi kaydedilmiştir. Prob ile doku fantomuna akım gönderilmiş ve ortamın iletkenlik bilgisi elde edilmiştir. Bulgular: Biyoimpedans iğne probu ile elde edilen özel sinyaller ile parçacık boyutu ayrımı gerçekleştirilmiştir. Doku fantomundaki parçacık boyut bilgisi 50 kHz direnç değeri ile değerlendirilmiştir. Polistiren mikroküreciklerden alınan biyoimpedans spektrumlarına göre parçacık boyutunun artmasıyla direnç değeri artış göstermiştir. Sonuç: Probun doku fantomundaki parçacık boyut ayrımını doğru bir şekilde belirlemesi ileriki aşamalarda hastalardan alınan beyin omurilik sıvılarında kültüre gerek duyulmadan Escherichia coli veya Acinetobacter baumanni gibi farklı boyutlara sahip mikroorganizmaların varlığını gerçek zamanlı teşhis etme potansiyeli barındırmaktadır. Ayrıca probun hücre kültürü çalışmalarında da hücre sayısını ve ayrımını yapabilme olasılığı bulunmaktadır.

Particle Size Detection By Bioimpedance Needle Probe

Objectives: The aim of this study was to test the Bioimpedance probe needle on in-vitro tissue phantom. Study Design: Bioimpedance probe needle that was connected to a bioimpedance measurement device to record spectra in the kHz range. The probe consists of an 18Gauge 1.2x89 mm spinal needle. The probe was used for obtaining impedance information about the polystyrene microspheres which were in different diameters; 1, 2 and 4 micrometers. The current was transferred to the phantom and conductivity information was obtained by the probe. Results: Bioimpedance probe needle allows the discrimination of different particle sizes based on their specific signatures. The sensitivity of the probe to particle size in tissue phantom was tested during the study. Spectroscopic data were evaluated with resistance values at 50 kHz and the information about the particle size in the tissue phantom was obtained. Bioimpedance spectra results which were obtained from polystyrene microspheres showed that the resistance values were increasing while the size of the particle was enlarging. Conclusion: Due to the highly accurate differentiation of particle size in the tissue phantom, our probe has the potential to be used in the rapid detection of Escherichia coli or Acinetobacter baumannii which have different sized microorganisms in the tapped cerebrospinal fluid without being cultured in real time. In addition, it is possible for the probe to perform cell counting and differentiation in cell culture studies.

Keywords:

Bioimpedance, spectroscopy, particle size needle,

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