Anaerop Kan Kültür Şişelerinin Rutin Kullanımının Değerlendirilmesi

Amaç: Rutin kan kültürü uygulamalarında farklı protokoller uyarınca 2 aerop şiş e ya da 1 aerop, 1 anaerop şişeden oluşan kan kültür setleri kullanılabilmektedir. Aerop kan kültür şişeleri ile birlikte anaerop kan kültür şişelerinin kullanıldığı kan kültür setlerinde fakültatif anaerop bakterilerin yalnızca aerop şişelerin kullanıldığı kan kültürlerine göre daha iyi üredikleri gösterilmiştir. Kan kültürlerinde anaerop üremeleri saptayabilmek ve fakültatif anaerop bakterilerin de saptanabilirliğinin arttırılması amacı ile anaerop kan kültür şişelerinin rutin kullanımının uygunluğu araştırılmıştır

Evaluation of Routine Usage of Anaerobic Blood Culture Bottles

Purpose: Blood culture sets consisting of 2 aerobic bottles or 1 aerobic and 1 anaerobic bottles could be used according to different protocols in routine blood culture applications. Better growth rates for facultative anaerobic bacteria were detected in aerobic and anaerobic bottle containing blood sets when compared to only aerobic bottle containing sets. In this study the convenience of routine usage of anaerobic blood culture bottles was evaluated for detection of anaerobic bacteria growth and increase the detection rate of facultative anaerobic bacteria

Keywords:

blood, culture anaerobic bacteria, Gram negative facultatively anaerobe rods,

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Dorsher CW, Rosenblatt JE, Wilson WR, Ilstrup DM. Anaerobic bacteremia: decreasing rate over a 15 year period. Rev Infect Dis 1991; 13: 633-6.

Sharp S. Routine anaerobic blood cultures: still appropriate today? Clin Microbiol Newsl 1991; 13: 179-81.

Wilson ML, Weinstein MP, Mirrett S, et al. Controlled evaluation of BacT/Alert standard anaerobic and FAN anaerobic blood culture bottles for the detection of bacteremia and fungemia. J Clin Microbiol 1995; 33: 2265-70.

Grohs P, Maniardi JL, Podglajen I, et al. Relevance of routine use of the anaerobic blood culture bottle. J Clin Microbiol 2007; 45: 2711-5.

Thorpe TC, Wilson ML, Turner JE, et al. BacT/Alert; an automated colorimetric microbial detection system. J Clin Microbiol 1990; 28:1608-12.

Flayhart DA, Borek AP, Wakefield T, Dick J, Carroll KC. Comparison of BACTEC PLUS blood culture media to BacT/Alert FA blood culture media for detection of bacterial pathogens in samples containing therapeutic levels of antibiotics. J Clin Microbiol 2007; 45: 816-21.

Zaidi AK, Knaut AL, Mirrett S, Reller LB. Value of routine anaerobic 210 blood cultures for pediatric patients. J Pediatr 1995; 127: 263-8.

Gransden WR, Eykyn SJ, Phillips I. Anaerobic bacteremia: declining rate over a 15-year period. Rev Infect Dis 1991; 13: 1255-6.

Murray PR, Traynor P, Hopson D. Critical assessment of blood culture techniques: analysis of recovery of obligate and facultative anaerobes, strict aerobic bacteria, and fungi in aerobic and anaerobic blood culture bottles. J Clin Microbiol 1992; 30: 1462-8.

Pottumarthy S, Morris AJ. Assessment of the yield of anaerobic blood cultures. Pathology 1997; 29: 415-7.

Ortiz E, Sande MA. Routine use of anaerobic blood cultures: are they still indicated? Am J Med 2000; 108: 445-7.

Bannister EB, Woods GL. Evaluation of routine anaerobic blood cultures in the BacT/Alert blood culture system. Am J Clin Pathol 1995; 104: 279-82.

Cornish N, Kirkley BA, Easley KA, Washington JA. Reassessment of the incubation time in a controlled clinical comparison of the BacT/Alert aerobic FAN bottle and standard anaerobic bottle used aerobically for the detection of bloodstream infections. Diagn Microbiol Infect Dis. 1998; 32: 1-7.

Morris AJ, Wilson ML, Mirret S, Reller LB. Rationale for selective use of anaerobic blood cultures. J Clin Microbiol 1993; 31: 2110-13.

Sharp SE, McLaughlin JC, Goodman JM, et al. Clinical assessment of anaerobic isolates from blood cultures. Diagn Microbiol Infect Dis 1993; 17: 19-22.

Borahan SE. Hastanemizde 2002-2006 yılları arasında reanimasyon dışı kliniklerin kan kültür sonuçlarının değerlendirilmesi.(Uzmanlık tezi). Sağlık Bakanlığı Şişli Etfal Eğitim ve Araştırma Hastanesi Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği.2007; İstanbul. 26-7.

Riley JA, Heiter BJ, Bourbeau PP. Comparison of recovery of blood culture isolates from two BacT/ALERT FAN aerobic blood culture bottles with recovery from one FAN aerobic bottle and one FAN anaerobic bottle. J Clin Microbiol. 2003; 421: 213-7.

Kiremitçi A, Türkkan AA, Akgün Y, Durmaz G, Kaşifoğlu N. Klinik örneklerden anaerop bakterilerin soyutlanması ve antibiyotik duyarlılıklarının belirlenmesi. ANKEM Derg. 2008; 22: 132-144.

Guerra-Romero L, Telenti A, Thompson RL, Roberts GD. Polymicrobial fungemia: microbiology, clinical features, and significance. Rev Infect Dis 1989; 11: 208-12.

Horn R, Wong B, Kiehn TE, Armstrong D. Fungemia in a cancer hospital: changing frequency, earlier onset, and results of therapy. Rev Infect Dis 1985; 7: 646-54.

Klein JJ, Watanakunakorn C. Hospital acquired fungemia: its natural course and clinical significance. Am J Med 1979; 67: 51-8.

Pfaller MA. Nosocomial Candida infections. Curr Opin Infect Dis 1988; 1: 764-71.

Wenzel RP, Pfaller MA. Candida species: emerging hospital bloodstream pathogens. Infect Control Hosp Epidemiol 1991; 12: 524-5.

Whimbey E, Kiehn TE, Brannon P, Blevins A, Armstrong D. Bacteremia and fungemia in patients with neoplastic disease. Am J Med 1987; 82: 723-30.