Malathionun Gammarus pulex (Tatlı Su Amphipodu)’te Asetilkolinesteraz Aktivitesi Üzerine Akut Etkisi

Bu çalışmada, organofosfat insektisit malathionun subletal konsantrasyonlarının Gammarus pulex'teki AChE aktivitesi üzerine akut (24 ve 48 saat) etkisi incelenmiştir. Bu amaçla, G. pulex'e malathionun iki farklı sublethal konsantrasyonu (0.1 ve 0.2 mg l-1) 24 ve 48 saat boyunca uygulandı. 24 ve 48 saat malathion maruziyetinin sonunda G. pulex örnekleri alındı. Alınan örneklerde AChE enzim aktivitesi ve protein düzeyi belirlenerek, spesifik AChE enzim aktivitesi hesaplandı. Malathionunun sublethal konsantrasyonları G. pulex'te AChE aktivitesininin zamana bağlı artan inhibisyonuna neden oldu. 0.1 mg l-1 malathion konsantrasyonuna maruz kalan grupta, AChE'nin inhibisyonu, kontrol ile karşılaştırıldığında, 24 saatte %50 ve 48 saatte %74 olarak tespit edildi. Benzer şekilde, 0.2 mg l-1 malathion konsantrasyonuna maruz kalan grupta da kontrol ile karşılaştırıldığında sırasıyla 24 ve 48 saatte % 60 ve % 68 AChE inhibisyonu gözlendi. Sonuç olarak, G. pulex'in malathiona 24 ve 48 saat boyunca maruz kalması AChE aktivitesinin yüksek oranda inhibe edilmesine neden olmuştur. Ayrıca, G.pulex'teki % 74'e kadar AChE inhibisyon seviyelerinin akut ölüme neden olmadığını ve G. pulex'teki AChE aktivitesinin ölçümünün akut malathion maruziyetinin ve etkilerinin biyobelirteci olacağını göstermektedir.

Anahtar Kelimeler:

Asetilkolinesteraz aktivitesi, Gammarus pulex, Malathion, organofosfatlar.

The Acute Effect of Malathion on Acetylcholinesterase Activity in Gammarus pulex (Freshwater Amphipoda)

In this study, the acute (24 and 48 h) effect of sublethal concentrations of malathion, organophosphates insecticide, on the AChE activity in Gammarus pulex was investigated. For this purpose, two sublethal concentrations of malathion (0.1 and 0.2 mg l-1) were applied to G. pulex for 24 and 48 h. After 24 and 48 hours of malathion exposure, G. pulex samples were taken. In the samples taken, AChE enzyme activity and protein level were determined and specific AChE enzyme activity was calculated.Sublethal concentrations of malathion caused time-dependent increased inhibition of AChE activity in G. pulex. In the group exposed to 0.1 mg l-1 malathion concentration, inhibition of AChE was detected as 50% at 24 h and 74% at 48 h, compared to control. Similarly, in the group exposed to 0.2 mg l-1 malathion concentration, 60% and 68% AChE inhibition at 24 and 48 h were observed, respectively, compared to the control.As a result, acute exposure of G. pulex to malathion for 24 and 48 h caused in the high rate inhibition of the AChE activity. Further, the results show that up to 74% AChE inhibition levels in G.pulex do not cause acute death, and measurement of AChE activity in G. pulex will be the biomarker of acute malathion exposure and effects.

Keywords:

Acetylcholinesterase activity, malathion, organophosphates, Gammarus pulex,

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