Döllenmemiş Yumurtaların Kısa Süreli Muhafazasını Takiben Koi Sazanı (Cyprinus carpio) Embriyolarının Kuluçkadan Çıkış Başarısı

Bu çalışma, farklı medyumların ve muhafaza sürelerinin, döllenmemiş yumurtaların kısa süreli muhafazasını takiben koi sazanı (Cyprinus carpio) embriyolarının kuluçkadan çıkış başarısını belirlemek amacıyla yürütülmüştür. 200 adetlik yumurta kümeleri 15 ml’lik petri kutuları içerisinde 20 ml’lik farklı medyumlarla (Cognie, Kurokura, Zhan-Liu) ve Ovaryum sıvısı ile muamele edilerek 20C’de 60, 120 ve 180 dk süre ile muhafaza edilmişlerdir. Her muhafaza süresinin sonunda, her petri kutusundaki yumurtalar 100 µl sperma ile döllenmiştir. En yüksek ortalama fertilizasyon ve kuluçka çıkış oranı, Ovaryum sıvısında 60 dk muhafaza edilen yumurta örneklerinde sırasıyla %68 ve %27 olarak belirlenmiştir. Bu çalışmadan elde sonuçlar, koi sazanı yumurtalarının 60-180 dk muhafaza edilmesinde en uygun sulandırıcının Ovaryum sıvısı olduğunu göstermiştir.

Anahtar Kelimeler:

Cyprinus carpio, kısa süreli muhafaza, yumurta

Hatching Success of Koi Carp (Cyprinus carpio) Embryos Following Short-Term Storage of Unfertilized Eggs

The present study was carried out to determine effect of different mediums and storage periods on fertilization and hatching success of koi carp (Cyprinus carpio) embryos following short-term storage of unfertilized eggs. The batches of about 200 pooled eggs treated with 20-ml three different artificial mediums (Cognie, Kurokura, Zhang-Liu) and ovarian fluid in 15-cm petri dishes, were stored at 20⁰C for 60, 120 or 180 min. Eggs were fertilized by adding 100 µl sperm in each petri dishes at the end of the each storage periods. The mean highest fertilization and hatching rates were determined as 68% and 27% with the egg samples stored for 60 min in Ovarian fluid respectively. Results obtained from this study indicate that Ovarian fluid is the most suitable solution for the short-term storage (60-180 min) of koi carp eggs.

Keywords:

Cyprinus carpio, short-term preservation, egg,

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