Nemrut Krater Gölünden (Bitlis/Türkiye) İzole Edilen Chlorella sp. Mikroalginde H2O2 Uygulaması ile Triaçilgliserol Üretiminin Artırılması

Bu çalışmada Nemrut volkanik gölünden izole edilen bir Chlorella sp. mikroalginde oksitatif stresin düzeyine bağlı olarak triaçilgliserol miktarında ve formasyonundaki değişiklikler incelenmiştir. Bu amaçla mikroalglerde oksidatif stresi uyarmak için büyüme ortamlarına 1µM, 5µM, ve 20µM yoğunluklarda Hidrojen peroksit (H2O2) uygulanmıştır. Hidrojen peroksituygulaması ilk günden itibaren büyümede önemli düzeyde baskılanmaya sebep olmuştur. Uygulamanın ilk 5 gününde büyümedeki baskılanma artmış, takip eden günlerde 1µM ve 5µM H2O2 uygulanan gruplarda büyüme yeniden gelişmiş ve baskılanma kalkmıştır. Mikroalglerin H2O2 içerikleri, ugulanan H2O2 yoğunluğu ile orantılı şekilde artmıştır. Mikroalglerin klorofil ve karotenoid içerikleri H2O2 uygulanan gruplarda konsantrasyon ile doğru orantılı olarak ilk 24 saatte artmış, daha sonra da lineer olarak azalmıştır. Mikroalglerin triaçilgliserol içerikleri özellikle 5uM H2O2 uygulamasına cevapta ilk 24 saatte yaklaşık olarak 2.1 kat artış ile en yüksek düzeyde gerçekleşmiştir. Floresans görüntüleme ile elde edilen sitoplazmik lipid cisimi üretimindeki artış da bu veriyi desteklemiştir. Böylece, bu çalışma ile elde edilen sonuçlar kısa süreli ve düşük yoğunlukta H2O2 uygulamasının mikroalglerden biyodizel hammaddesi olan triaçilgliserol üretiminde kullanılabilecek bir yaklaşım olduğunu göstermektedir.

Enhancement of Triacylglycerol Production via H2O2 Application in Chlorella sp. Isolated from Nemrut Crater Lake (Bitlis/Turkey)

In this study, oxidative stress depended changes in lipid production and formation in Chlorella sp. was analyzed. The strain was isolated from Lake Nemrut which is a volcanic lake located in Bitlis province. In order to induce oxidative stress in microalgae 1µM, 5µM, and 20µM hydrogen peroxide (H2O2) were added into the growth media. Hydrogen peroxide application caused a significant decrease in growth starting from the first day of incubation. Following decrease of the growth for first five-days, microalgae started to grow up as a response to 1µM and 5µM H2O2 application. The H2O2 contents of microalgae increased in proportion to the applied H2O2 concentation. Total chlorophyll and carotenoid contents showed a gradual increase during first 24 hours of H2O2 application, and then a linear decrease was followed afterwards. A maximum of 2.1 fold increase in triacylclygerole content of microalgae was recorded after 24 hours of 5uM H2O2 aplication.  Fluorescence imaging of cytoplasmic lipid bodies support this datum. Thus, results of this study showed that short-term and low-density H2O2 application might be used as a potential approach to induce microalgal triacylglycerol production as a biodiesel feedstock.

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Acta Aquatica Turcica-Cover
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 1988
  • Yayıncı: Yunus Ömer BOYACI
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