Türkiye’de Yetiştirilen Bazı Sığır Irklarında Akirin 2 Gen Polimorfizminin PCR-RFLP Yöntemi ile Belirlenmesi

Bu çalışmada Türkiye’de yetiştirilen Simental (n=75), İsviçre Esmeri (n=75), Holstein (n=100), Doğu Anadolu Kırmızısı (DAK) (n=40) ve Boz Irk (n=40) sığırlarında Akirin 2 geninde bulunan c.*188G>A tek nokta polimorfizminin (SNP) belirlenmesi amaçlanmıştır. Bu çalışmada, c.*188G>A polimorfizmi yönünden genotipleme için polimeraz zincir reaksiyonunu takiben yapılan restriksiyon parçacık büyüklük polimorfizimi (PCR-RFLP) yöntemi kullanılmıştır. Akirin 2 genindeki hedef bölge, PCR ile çoğaltıldıktan sonra elde edilen PCR ürünleri FoKI endonükleaz enzimi ile kesilmiştir. Çalışmada c.*188G>A tek nokta polimorfizmi için A ve G olarak adlandırılan iki allel belirlenmiştir. En yüksek A allel frekansı Simental ırkında (0.27), en yüksek G allel frekansının ise DAK ırkında (0.93) olduğu görülmüştür. Ayrıca, en yüksek AA ve AG frekansı Simental ırkında (0.07 ve 0.39), en yüksek GG genotip frekansı ise DAK ırkında (0.85) belirlenmiştir. Yapılan Ki-kare analiz sonuçlarına göre incelenen beş ırkında c.*188G>A polimorfizmi yönünden Harrdy-Weinberd dengesinde (HWE) oldukları gözlenmiştir. Bu çalışma Türkiye’de yetiştirilen Simental, İsviçre Esmeri, Holstein, DAK ve Boz Irk sığırlarında Akirin 2 geninden bulunan c.*188G>A polimorfizmi yönünden genotiplendirilmelerinin yapıldığı ilk çalışmadır. Sonuç olarak, Türkiye’de yetiştirilen yerli ve Avrupa orijinli sığır ırklarında mermerleşme skoru gibi et kalite özelliklerinin iyileştirilmesinde Akirin 2 genindeki c.*188G>A polimorfizminin kullanılabileceği düşünülmektedir.

Anahtar Kelimeler:

Akirin 2, Genetik Belirteç, RFLP, Sığır

Detection of Akirin 2 Gene Polymorphism with PCR-RFLP Method in Some Cattle Breeds Reared in Turkey

The purpose of this study was to determine a single nucleotide polymorphism (SNP) c.*188G>A at Akirin 2 gene in the Simmental (n=75), Brown Swiss (n=75), Holstein (n=100), East Anatolian Red (EAR) (n=40) and Turkish Grey (n=40) cattle breeds in Turkey. In order to determine the c.*188G>A polymorphism, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method was performed in this study. Target region of Akirin 2 gene was amplified using PCR method and the PCR products were digested using by FoKI endonuclease enzyme. In this study, two types of alleles (A and G) for c.*188G>A polymorphism of Akirin 2 gene were observed. The G allele frequency was found the highest in EAR cattle breed (0.93) and the A allele frequency was found the highest in Simmental cattle breed (0.27). Furthermore, the AA and AG genotype frequencies were found to be highest in Simmental breed (0.07 and 0.39 respectively), while the highest frequency of GG genotype was found in EAR (0.85) breed. According to the results of the chi-square test, it was showed that the investigated five breeds were in Hardy-Weinberg equilibrium for the c.*188G>A polymorphism. The present study is the first attempt that investigates c.*188G>A polymorphism at Akirin 2 gene in these cattle breeds (Simmental, Brown Swiss, Holstein, EAR and Turkish Grey) raised in Turkey. In conclusion, it is considered that the c.*188G>A polymorphism in Akirin 2 gene can be used in order to improve meat quality traits such as marbling score of Turkish native and European origin cattle breeds reared in Turkey.

Keywords:

Akirin 2, Genetic Marker, RFLP, Cattle,

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