Tavşanlarda Mistletoe Lektin-I’in Farklı Dozlarının Tümör Nekroz Faktör-α, Nitrik Oksit, Total Antioksidan ve Oksidan Kapasite Düzeylerine Etkisi

Mistletoe lektin-I (ML-I)’in, farklı dozlarının tümör nekroz faktör-α (TNF-α), nitrik oksit (NO), total antioksidan ve oksidan kapasite (TAK, TOK) düzeylerine etkisinin araştırıldığı çalışmada, her birinde 7 adet tavşan bulunan, birisi kontrol olmak üzere 4 grup oluşturuldu. Gruplara sırasıyla 0.5, 1 ve 2 ng/kg ML-I intraperitonal (i.p.) olarak enjekte edildi. Enjeksiyonu takiben 1., 6., 12. ve 24. saatlerde kan örnekleri alınarak plazmaları elde edildi. 0.5 ng/kg ML-I verilen grupta TNF-α düzeyinin bütün saatlerde kontrol ve diğer lektin gruplarına göre yüksek olmakla birlikte 1. saatte en yüksek değerine ulaştığı (p<0.01) saptandı. 0.5 ve 1 ng/kg lektin dozlarının 1. saatte NO düzeyini azalttığı (p<0.05), 1 ve 2 ng/kg lektin dozlarının 12. saatte NO düzeyini arttırdığı (p<0.05), gruplar arasında istatistiksel olarak bir farkın oluşmadığı gözlendi. 0.5 ng/kg ML-I verilen gruptan alınan numunelerde, 1 saat sonra elde edilen TAK değerlerinin 6, 12 ve 24. saatlere göre istatistiksel olarak yüksek (p<0.05) olduğu, TOK düzeylerinde istatistiksel olarak bir değişim olmadığı gözlemlendi. 0.5 ng/kg dozundaki ML-I’in TNF-α ve NO düzeylerinde değişiklik meydana getirmesi, lektinin akut durumlarda antioksidan özellik gösterebileceği kanaatini oluşturdu.

Anahtar Kelimeler:

Mistletoe Lektin-I, TNF-α, Nitrik Oksit, Total Antioksidan ve Oksidan Kapasite

Effect of Different Doses of Mistletoe Lectin-I on the Levels of Tumor Necrosis Factor-α, Nitric Oxide, Total Antioxidant and Oxidant Capacity in Rabbits

This paper aimed to investigate the effect of different doses of mistletoe lectin-I (ML-I) on the levels of tumor necrosis factor-α (TNF-α), nitric oxide (NO), total antioxidant and oxidant capacity (TAC, TOC). The rabbits were divided into 4 groups (Control, Group-I, II and III) each group contained 7 rabbits. 0.5, 1 and 2 ng/kg of ML-I were administered to treatment groups via intraperitoneal route, respectively. Plasmas of blood samples obtained in the 1st, 6st, 12th and 24th hours following injection were seperated. TNF-α level in the group treated with ML-I (0.5 ng/kg) was higher than that of control and other lectin receiving groups at all hours, the highest value of TNF-α was reached at 1st hour (p<0.01). 0.5 and 1 ng/kg doses of lectin decreased NO level at 1st hour compared to control group (p<0.05), 1 and 2 ng/kg doses of lectin increased NO levels (p<0.05) at 12th hour, no statistically significant difference was found between the groups. In addition, TAC levels in samples obtained from the group given 0.5 ng/kg ML-I after 1 hour was statistically higher (p<0.05) than the samples taken at 6th, 12th and 24th hours. No statistically significant change was found in the level of TOC among lectin receiving groups. In conclusion, ML-I at 0.5 ng/kg dose caused alterations in the levels of TNF-α and NO and showed an acute antioxidant effect.

Keywords:

Mistletoe Lectin-I, TNF-α, Nitric Oxide, Total Antioxidant and Oxidant Capacity,

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