Simten YESILMEN , Neval Berrin ARSERIM , Nurettin ISIK , Hasan ICEN

Diyarbakır Bölgesindeki Sığırlarda Patojenik Leptospira spp Prevalansının Real-time PCR Yöntemi ile Tespiti

Bu çalışmada Diyarbakır’daki kesimhanelerde kesilen sığırların kan ve idrar örneklerinde real time PCR yöntemiyle patojenik Leptospira prevalansının ortaya konulması amaçlanmıştır. Bu çalışmanın materyalini üç büyük kesimhanedeki sığırlardan 96 sığırdan toplanan idrar ve kan örnekleri oluşturdu. Bu örneklerde patojen Leptospiraların varlığı real-time PCR yöntemiyle araştırıldı. Patojenik Leptospiraları etkenlerini varlığını ortaya koymak için hap 1 geninin tespit etme temeline dayanan yöntem kullanılmıştır. Leptospira şüpheli 96 sığırdan elde edilen idrar örneklerinden 9 (%9.4) adetinde hap1 geninin varlığı tespit edilmiştir. Aynı hayvanlardan alınan kan örneklerinde pozitifliğe rastlanılmamıştır. Bu nedenle, taşıyıcı hayvanların erken teşhisi, leptospiral enfeksiyonun diğer hayvanlara ve insanlara bulaşmasının önlenmesi bakımından son derece önemlidir

Anahtar Kelimeler:

Leptospira, Real-Time PCR, Hap 1, Sığır

Diyarbakır Bölgesindeki Sığırlarda Patojenik Leptospira spp Prevalansının Real-time PCR Yöntemi ile Tespiti

Keywords:

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Adler B, Moctezuma A (2010). Leptospira and leptospirosis. Vet Microbiol, 140, 287–296.
Aslantas O, Ozdemir V (2005). Determination of the seroprevalence of leptospirosis in cattle by MAT and ELISA in Hatay, Turkey. Turk J Vet Anim Sci, 29, 1019-1024.
Baer R, Turnberg W, Yu D, Wohrle R (2010). Leptospirosis in a small animal veterinarian: reminder to follow standardized infection control procedures. Zoonoses public health, 57, 281–284.
Bal AE, Gravekamp C, Hartskeerl TRA, De Meza-Brewster J, Korver H, Terpstra WJ (1994). Detection of leptospires in urine by PCR for early diagnosis of leptospirosis Journal Of Clinical Microbiology, 32(8), 1894- 1898.
Bomfim MRQ, Ko A, Koury MC (2005). Evaluation of the recombinant LipL32 in enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis. Vet Microbiol, 109, 89–94.
Bomfim MRQ, Koury MC (2006). Evaluation of LSSP-PCR for identification of leptospira spp. in urine samples of cattle with clinical suspicion of leptospirosis. Vet Microbiol, 118, 278–288.
Bomfim MRQ, Barbosa-Stancioli EF, Koury MC (2008). Detection of pathogenic leptospires in urine from naturally infected cattle by nested PCR. The Veterinary Journal, 178, 251–256.
Branger C, Blanchard B, Fillonneau C, Suard I, Aviat F, Chevallier B, Andre´-Fontaine G (2005). Polymerase chain reaction assay specific for pathogenic Leptospira based on the gene hap1 encoding the hemolysis-associated protein-1 FEMS Microbiology Letters, 243 437– 445.
Cai HY, Hornby G, Key DW, Osuch MR, Maxie MG (2002). Preliminary study on differentiation of Leptospira grippotyphosa and Leptospira sejroe from other common pathogenic leptospiral serovars in canine urine by polymerase chain reaction assay. J Vet Diagn Invest, 14,164– 168.
Cetinkaya B, Ertas HB, Muz A, Ongor H, Kalender H Ozdemir V (1999). Determination of seroprevalence of leptospirosis in cattle in Elazig. Turk J Vet Anim Sci, 23(3), 633-639.
Cetinkaya B, Ertas H.B, Ongor H, Muz A (2000). Detection of leptospira species by Polymerase Chain Reaction (PCR) in urine of cattle. Turk J Vet Anim Sci, 24, 123–130.
Ertas HB, Çetinkaya B, Muz A, Ongor H, Ozdemir V, Yazioglu N (2002). Determination of the seroprevalence of leptospira in cattle by MAT and ELISA Turk J Vet Anim Sci, 26 1415-1420
Fearnley C, Wakeley PR, Gallego-Beltran J, Dalley C, Williamson S, Gaudie C, Woodward MJ (2008). The development of a real-time PCR to detect pathogenic Leptospira species in kidney tissue. Research in Veterinary Science, 85, 8–16
Gazyagci S, Yildirim M, Kaygusuz S (2010). Investigation on efficacy of a commercial vaccine for treatment of leptospirosis in cattle. Journal of Animal and Veterinary Advances, 9 (10), 1531 -1533
Gumussoy KS, Ozdemir V, Aydin F, Aslan O, Atabek E, lca T, Doğan H O, Duman Z, A Ozturk (2009). Seroprevalence of bovine leptospirosis in Kayseri, Turkey and detection of leptospires by Polymerase Chain Reaction. Journal of Animal and Veterinary Advances, 8 (6), 1222-1229.
Ikiz S, Ozgur Y, (2004). Detection of Leptospira interrogans antibodies by ELISA and Microscopic Agglutination Test (MAT) in cattle in Trakya district and bacteriological studies on leptospirosis in slaughtered cattle. Istanbul Univ. Vet. Fak. Derg, 30, 99-111.
Jafari Dehkordi A, Shahbazkia HR, Ronagh N (2011). Evaluation of pathogenic serovars of Leptospira interrogans in dairy cattle herds of Shahrekord by PCR. Iran. J. Microbiol, 3 (3), 135-139.
Kocabiyik, AL, Cetin C (2004). Bovine leptospirosis in South Marmara region of Turkey: A serological survey. Rev.Med. Vet, 155, 606-608.
Levett PN, Morey RE, Galloway RL, Turner DE, 7. Steigerwalt AG, Mayer LW. (2005) Detection of pathogenic leptospires by real-time quantitative PCR. J Med Microbiol, 54, 45-49.
Lilenbaum W, Souza GN (2003). Factors associated with bovine leptospirosis in Rio de Janeiro, Brazil Res.Vet. Sci, 75, 249-251.
Lucchesi PMA, Arroyo GH, Etcheverría AI, Parma AE, Seijo AC (2004). Recommendations for the detection of Leptospira in urine by PCR. Revista da Sociedade Brasileira de Medicina Tropical 37(2),131-134.
Moinet M (2008). Étude comparative de la leptospirose chez le vison d’Europe [Mustela lutreola] et les autres petits carnivores sauvages du sud-ouest de la France. Thesis for Doctor of Veterinary Medicine, submitted to the University of Nantes, France, 1-108,
OIE Terrestrial Manual (2008). Leptospirosis. Chapter 2.1.9, 252-259.
Ozdemir V, Erol E (2002). Leptospirosis in Turkey. Vet. Rec, 23, 248-249.
Prapong S, Suwanchareon D, Tohmee N (2003). Genotyping Survey the Leptospira in Bovine Urine Samples in Thailand by PCR-Based Method: A Non Matching with the Antibody Titer by MAT. The 11th International Symposium of the World Association of Veterinary Laboratory Diagnosticians and OIE Seminer on Biotechnology November, 9-13
Sahin M, Aydin F, Ozdemir V, Genç O, Guler MA (2002). Serological survey of bovine leptospirosis in Kars and Ardahan provinces. Turk J Vet Anim Sci, 26, 17-25.
Sakhaee E, Abdollahpour GhR, Bolourchi M, Hasani Tabatabayi AM, Sattari Tabrizi S (2007). Serologic and bacteriologic diagnosis of bovine leptospirosis in Tehran suburb dairy farms. Iranian Journal of Veterinary Research, University of Shiraz, 8,4(21), 325-332.
Taylor MJ, Ellis WA, Montgomery JM, Yan KT, McDowell SWJ, Mackie DP (1997). Magnetic immuno capture PCR assay [MIPA]: detection of Leptospira borgpetersenii serovar hardjo. Vet Microbiol, 56(1-2), 135- 145.