Staphylococcus epidermidis ve Staphylococcus aureus antibiyotik direncine sebep olan biyofilm oluşumunun belirlenmesi için kullanılan metotların kıyaslanması

ÖzAmaç: Bu çalışma, tedavileri imkansız ve zor olan, konak ve yabancı cisim ilişkili infeksiyonlar gibi tekrarlayabilen infeksiyonları olan Staphylococcus aureus and Staphylococcus epidermidis gibi Stafilokoklar  tarafından oluşturulan biyofilmin belirlenmesinde kullanılan MtP, TM ve CRA tarama metotlarını kıyaslamak için gerçekleştirildi. Yöntem ve Gereç: Bu çalışmada, 121 izolat kullanıldı ve altın standart metot olarak MtP metot kullanıldı. Sensitivite, spesifisite, pozitif tahmin değeri ve negatif tahmin değeri parametreleri hesaplandı. Bulgular: TM ve CRA metotlarının sensitivite, spesifisiteleri, sırasıyla %97, %100 ve %87, %94 dür. Tartışma: Bu çalışma Tüp Metodun CRA metottan daha güvenilir olduğunu gösterdi. TM, laboratuvarlarda biyofilm oluşturan bakteri identifikasyonu için ana tarama testi olarak tavsiye edilebilir. Güvenilir biyofilm belirleme metodunun kullanımı ile yanlış tanılar ve tekrarlayan infeksiyonlar önlenebilir.

The comparison of methods used for the detection of biofilm formation that cause antibiotic resistance of Staphylococcus epidermidis and Staphylococcus aureus

                                                                   AbstractAim: This study was performed to compare MtP, TM and CRA screening methods used for the detection of biofilm formation by Staphylococcus spp. such as Staphylococcus aureus and Staphylococcus epidermidis of which treatment can be impossible and hard, and infections such as host and indwelling device-associated infections can be recurrent.Materials and Methods: In this study 121 isolates were used and MtP method was used as gold standart method. Sensitivity, specificity, positive predictive value and negative predictive value parameters were calculated.Results: The sensitivity, specificity of TM and CRA methods were 97%, 100% and 87%, 94%, respectively. Conclusion: This study revealed that TM is more reliable method than CRA method. TM can be recognized as the main screening method for the identification of biofilm producer bacteria in the laboratories. By the usage of reliable biofilm detection method, wrong diagnosises and recurrent infections can be prevented.

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Ortadoğu Tıp Dergisi-Cover
  • Başlangıç: 2009
  • Yayıncı: MEDİTAGEM Ltd. Şti.
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