Acil servise başvuran çocuk hastalarda kısa süreli açlık ile oksidatif stres arasındaki ilişkinin değerlendirilmesi

Amaç: Sağlıklı bir yaşamın devam ettirilebilmesi için organizmanın oksidan antioksidan dengesinin korunması gereklidir. Oksidatif stresteki artış sonucunda oluşan serbest radikal türleri hücre zedelenmesine ve hücre ölümüne neden olur. Oksidatif stresin birçok hastalığın etiyolojisinde rolü olduğu bilinmektedir. Bu çalışmanın amacı radyolojik görüntüleme için kısa süre aç bırakılan çocuk hastaların iskemi modifiye albumin, tiyol ve disulfid serum düzeylerini belirleyerek, açlık ile oksidatif stres arasındaki ilişkiyi araştırmaktır.Gereç ve Yöntem: Çalışmamız Nisan ile Kasım 2018 tarihleri arasında gerçekleştirildi. Yaşları altı ay ile 6 yaş arasında değişen pediatrik hastalar dahil edildi. En az beş saat aç bırakılan pediatrik hastalar çalışma grubu olarak belirlendi, aç olmayan hastalar kontrol grubu olarak kabul edildi. Oksidatif stres parametreleri olarak iskemi modifiye albumin, tiyol ve disülfid serum düzeylerini Erel kalorimetrik yöntemi ile ölçüldü.Bulgular: En az beş saat aç olan hastaların ortalama ± SD nativ tiyol düzeyi 559,15± 58,98 μmol/L, total tiyol düzeyi 606,83± 61,35μmol/L, disülfid düzeyi 23,83± 5,58 μmol/L ve iskemi modifiye albumin düzeyi 0,72± 0,12 ABSU idi. Kontrol hastalarında ortalama± SD nativ tiyol düzeyi 528,80± 54,45 μmol/L, total tiyol düzeyi 580,17± 63,41 μmol/L, disülfid düzeyi 25,68± 8,07 μmol/L ve iskemi modifiye albumin 0,77± 0,15 ABSU idi. Disülfid/tiyol ve disülfid/total tiyol oranları aç bırakılan hasta grubunda konrol grubuna göre daha düşüktü, ayrıca gruplar arasında oranların ortalamaları açısından anlamlı bir istatistiksel farklılık saptanmadı.Sonuç: Kısa süre aç bırakılan çocuklarda oksidatif stres belirteçlerinin değişmediği ve dengesinin bozulmadığı görüşüne varıldı.

Anahtar Kelimeler:

açlık, çocuk, disülfid, iskemi modifiye albumin, tiyol, oksidatif stres

The relationship between short term fasting and oxidative stress in pediatric emergency patients

Aim: Healthy life is sustained by maintaining the balance between oxidative and antioxidant homeostasis in the living organism. During oxidation process free radical products lead to cellular damage and death. It is well known that oxidative stress plays role in the etiology of many diseases. We aimed to study the relationship between oxidative stress and short term fasting by measuring the serum levels of ischemia modified albumin, thiol, and disulphide in pediatrics patients admitted for radiological imaging.Material and Method: Our study was carried out between April and November 2018. We included children between 6 months and 6 years. Children who were fasting for more than 5 hours were assigned as the study group. Children who were not fasting were assigned as the control group. Serum ischemia modified albumin, thiol and disulfide were calculated using the colorimetric method of Erel’s.Results: In the study group, the mean± SD serum level of ischemia modified albumin, native thiol, total thiol and disulphide were 0.72± 0.12 ABSU, 559.15± 58.98 μmol/L, 606.83± 61.35 μmol/L and 23.83± 5.58 μmol/L respectively. In the control group, the mean ± SD serum level of ischemia modified albumin, native thiol, total thiol and disulphide were 0.77± 0.15 ABSU, 528.80± 54.45 μmol/L, 580.17± 63.41 μmol/L and 25.68± 8.07 μmol/L respectively. The disulphide /native thiol and disulphide / total thiol ratios were lower in the study group than the controls, but there were no significant differences between the two groups.Conclusion: Short term fasting does not change serum levels of oxidative stress biomarkers and dose not lead to inbalance in the oxidative stress hemeostasis in pediatric patients.

Keywords:

disulphide, fasting, ischemia modified albumin, pediatrics, thiol, oxidative stress,

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