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Usability is one of the factors that can affect the success of students where learning processes are supported by internet and web technologies. In this study, usability factors for effective design and usage of web based multimedia packages created for educational purposes are evaluated. The research, experiments and analysis are done in the Human–Computer Interaction Laboratory that resides in Computer Education and Instructional Technologies Department of Marmara University. Data for the experiment are collected by using screen records which contain eye and mouse motions. For the analysis of data, fixation count, sequence and dwell time values in gridded area of interest; fixation count, heat map and scanpath values in whole are considered. The results obtained from this study can be used for design and validation of computer-based materials like virtual class applications, learning objects, educational applications, training applications and educational games.