Pathogenic Vibrio Strains Isolated from Human Stool and Water Samples from Western Kenya

Öz Objective: Investigate the type of pathogenic Vibrio strains from water and stool samples collected from Migori, SonduMiriu, Nyando and Yala regions in Western Kenya. Methods: A total of 811 samples (596 water and 215 stool samples) were collected during the study periods of May to December 2013 and August to September 2014. Pathogenic Vibrio strains were identified through culturing in TCBS Agar, followed by oxidation, string and serological (polyvalent) tests, respectively. The PCR analysis was done using combined primers targeting Vibrionaceae 16SrRNA and species specific primers for V. vulnificus and V. cholerae. Results: The results showed the presence of V. vulnificus and V. cholerae. However, V. parahaemolyticus was not found in any of the samples. The PCR results for 16SrRNA, Vib 1, and Vib 2 showed polymorphism in the genes, this was an indication of cross combination of genes from more than one strain in one isolate. Conclusion: The study showed the presence of V. cholerae (Ogawa and Inaba) in water and human stool samples. Type B V. vulnificus was detected in the water sample collected from River Migori. This information is of essence in controlling and managing cholera in the western part of Kenya. J Microbiol Infect Dis 2016;6(1): 1-7


1. Atlas RM. Principles of microbiology: Bacterial diversity, 2nd edn. Boston: Fishback. 1997;17:80.

2. Mahmud ZH Neogi SB, Kassu A, et al. Occurrence, seasonality and genetic diversity of Vibrio vulnificus in coastal seaweeds and water along the Kii Channel, Japan. FEMS Microbiol Ecol. 2008;64:209-218.

3. Colwell RR. Global climate and infectious disease: the cholera paradigm. Science. 1996;274:2025-2031.

4. Pascual M, Rodó X, Ellner SP, et al. Cholera dynamics and El nino-Southern Oscillation. Science. 2000;289:1766-1769.

5. Kiiru JN, Saidi SM, Goddeeris BM, et al. Molecular characterisation of Vibrio cholerae O1 strains carrying an SXT/R391- like element from cholera outbreaks in Kenya: 1994-2007. BMC Microbiol. 2009;9:275.

6. Nair GB, Ramamurthy T, Bhattacharya SK, et al. Global dissemination of Vibrio parahaemolyticus serotype O3:K6 and its serovariants. Clin Microbiol Rev. 2007;20:39-48.

7. Scallan E, Hoekstra RM, Angulo FJ, et al. Foodborne Illness Acquired in the United States-major Pathogens. Emerg Infect Dis. 2014;17:7-15.

8. Jones MK, Oliver JD. Vibrio vulnificus: disease and pathogenesis. Infect Immun. 2009;77:1723-1733.

9. LVEMP II Consolidated Annual Report 2011-2012 http://lvemp.

10. Lutz C, Erken M, Noorian P, et al. Environmental reservoirs and mechanisms of persistence of Vibrio cholerae. Front Microbiol 2013;4:375.

11. Zhang XH, Austin B. Haemolysins in Vibrio species. J Appl Microbiol 2005;98:1011-1019.

12. Collin B, Rehnstam-Holm AS, Lindmark B, et al. The origin of Vibrio cholerae influences uptake and persistence in the blue mussel Mytilusedulis. J Shellfish Res. 2012;31:87-92.

13. Sakazaki R. Bacteriology of Vibrio and related organisms In: Barua D, Greenough WB, Cholera, 3rd edn. Planum Medical Books Company, New York 1992:37-55.

14. Nandi B, Ranjan KN, Sarmishtha MP, et al. Rapid method for species-specific identification of Vibrio cholerae using primers targeted protein OmpW. J Clin Microbiol 2000; 38:4145- 4151.

15. McLaughlin JC. Vibrio. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC,Yolken RH, eds. Manual of clinical microbiology, 1st edn. Washington, DC 1995:465-476.

16. Colwell RR, Huq A. Environmental reservoir of Vibrio cholerae. The causative agent of cholera. Ann. NY Acad Sci 1994; 740:44-54.

17. International Federation of Red Cross and Red Crescent Societies (2015) Kenya: Cholera Emergency Plan of Action (EPoA) Operation no MDRKE033. 2015-000013-ken.2015.

18. Thompson FL, Austin B, Swings J. The biology of Vibrios, 1st edn. ASM Press, Washington, DC 2006.

19. Jay JM, Loessner MJ, Golden DA. Modern Food Microbiology, 7th edn. Dennis Heldman, 2005.

20. Mazel D, Dychinco B, Webb VA, Davies J. A distinctive class of integron in the Vibrio cholerae genome. Science. 1998;280:605-608.

21. Nadja B, Silke B, Susanne D, et al. Genotypic Diversity and Virulence Characteristics of Clinical and Environmental Vibrio vulnificus isolates from the Baltic Sea Region. Appl Environ Microbiol. 2013;79:3570-3581.

22. Kita-Tsukamoto K, Oyaizu K, Shimidu U. Phylogenitic relationships of marine bacteria, mainly members of the family Vibrio cholerae, determined on the basis of 16S rRNA sequence. Int J Syst Bacteriol. 1993;43:8-19.

23. Morris JG. Non- 0 group 1 Vibrio cholerae: A look at the epidemiology of an occasional pathogen. Epidemiol Rev. 1990;12:179-191.

24. Oketch A, Kaluoch M. Cholera claims four more as toll goes up. 19 Feb 2015.

25. Kim MS, Jeong HD. Development of 16S rRNA targeted PCR methods for the detection and differentiation of Vibrio vulnificus in marine environments. Aquaculture. 2001;193:199- 211.