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In adolescence adolescents are facing a big transformation both in psychology and physiology, they need time to adapt during the course of transformation, and they are prone to generate negative thinking disposition if not well adapted, thereby affecting subsequent life. Based on the above critical reason, this study aims at describing and explaining effects of present situation and characteristics of adolescents' positive, negative thinking disposition on team collaboration disposition. This study adopted questionnaire method to collect data from design group students of a senior vocational school in central region, and a total of 157 valid student questionnaires were collected; used SPSS 18.0 to conduct Pearson correlation analysis and multiple regression empirical analysis, and carried out estimation and verification for structural relationships between each group of variables. The study results show that: (1) adolescents' positive thinking disposition and negative thinking disposition have a significant negative correlation, (2) adolescents' positive thinking disposition and negative thinking disposition respectively has a significant relationship with team collaboration disposition; (3) positive, negative thinking disposition has a significant predictability for team collaboration disposition.