KURKUMİN UYGULAMASININ YÜKSEK YAĞLI DİYETLE BESLENEN SIÇANLARIN TESTİS DOKUSUNDA OKSİDANANTİOKSİDAN SİSTEM ÜZERİNE ETKİSİ

üksek yağlı diyetin (YYD) neden olduğu oksidatif stresin testis fonksiyonlarını olumsuz yönde etkilediği bilinmektedir. Zerdeçal baharatının aktif bileşeni olan kurkuminin oksidan-antioksidan denge üzerine olumlu etkileri olduğu gösterilmiştir. Bu çalışmanın amacı; YYD birlikte alınan kurkuminin testiste oksidan-antioksidan denge üzerine olan etkisini incelemektir. Gereç ve Yöntem: Sprague-Dawley erkek sıçanlar dört gruba ayrılmış ve 16 hafta süreyle beslenmiştir. Birinci gruba kalorisinin %10’unun yağdan karşılandığı normal diyet, ikinci gruba kalorisinin %60’ının yağdan karşılandığı YYD, üçüncü gruba YYD’e karıştırılmış kurkumin (1g/kg yem) ve dördüncü gruba sadece normal diyet+kurkumin (1g/kg yem) verilmiştir. Deney süresi sonunda testis dokusu homojenize edilerek postmitokondriyal ve mitokondriyal fraksiyonlara ayrılmış; testiste reaktif oksijen türleri (ROS) düzeyleri florometrik yöntemle, malondialdehit (MDA) ve glutatyon (GSH) düzeyleri ile glutatyon peroksidaz (GPx), süperoksit dismütaz (SOD) ve glutatyon transferaz (GST) aktiviteleri spektrofotometrik yöntemlerle ölçülmüştür. Bulgular: YYD uygulaması, testiste postmitokondriyal ve mitokondriyal MDA düzeylerini arttırmış; sitozolik GSH düzeylerini ise azaltmıştır. YYD+Kurkumin uygulaması YYD’e bağlı artış gösteren MDA düzeylerini düşürmüş; GSH düzeyini ve GST aktivitesini ise arttırmıştır. Ancak sadece kurkumin uygulaması oksidan-antioksidan göstergeleri etkilememiştir. ROS düzeyleri ile SOD ve GPx aktiviteleri ise gruplar arasında anlamlı değişim göstermemiştir. Sonuç: Bulgularımız, YYD ile birlikte alınan kurkuminin testiküler oksidan-antioksidan dengeyi koruyucu olabileceğini göstermektedir. Ancak bu etkinin moleküler mekanizmalarının incelenmesi için ileri çalışmalar yapılması gerekmektedir.

Anahtar Kelimeler:

Kurkumin, Yüksek Yağlı Diyet, OksidanAntioksidan Sistem, Testis

EFFECT OF DIETARY CURCUMIN ON OXIDANT-ANTIOXIDANT STATUS IN TESTIS TISSUES OF RATS FED A HIGH-FAT DIET

Objective: High fat diet (HFD) induced oxidative stress is known to adversely affect testicular functions. Curcumin, which is an active ingredient of turmeric spice, has been shown to reduce lipid peroxidation and stimulate cellular antioxidant enzymes. The aim of this study is to investigate the effect of dietary curcumin on the testicular oxidant-antioxidant status in rats fed on a HFD. Material and Method: Male Sprague-Dawley rats were divided into four groups. Group 1 was fed with a control diet (10% of total calories from fat). Group 2 was fed with a HFD (60% of total calories from fat). Groups 3 and 4 received a HFD and a control diet with curcumin (1g/kg diet; w/w) respectively for 16 weeks. The testis tissue was homogenized and divided into postmitochondrial and mitochondrial fractions and the reactive oxygen species (ROS) levels were measured with the fluorometric method. The malondialdehyde (MDA) and glutathione (GSH) levels and glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione transferase (GST) activities were measured with spectrophotometric methods. Results: HFD supplementation increased postmitochondrial and mitochondrial MDA levels and decreased cytosolic GSH levels in testis. MDA levels decreased with curcumin supplementation. Moreover, GSH levels and GST activity increased. However, curcumin supplementation alone did not affect oxidant-antioxidant status. ROS levels, SOD and GPx activities did not change significantly between the groups. Conclusion: Our data has shown that curcumin supplementation with HFD may prevent testicular oxidant damage. However, further studies are needed to investigate the underlying molecular mechanisms.

Keywords:

Curcumin, High Fat-Diet, Oxidant-Antioxidant Status, Testis,

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