Serap KURUCA, Kadriye AKGÜN DAR, Ayşegül KAPUCU, Dilşad ÖZERKAN

1,2 DİMETİLHİDRAZİN İLE OLUŞTURULAN KOLON KANSERİ MODELİNDE ZEOLİT DESTEKLİ BESLENMENİN KANSER GELİŞİMİNE ETKİLERİ

Amaç: Çevre kirliliği ve 1,2-Dimetilhidrazin (DMH) gibi kimyasal maddelere maruz kalan bitki ve hayvanlara beslenmek insanlarda çeşitli hastalıklara yol açar. Bunlardan biri kolon kanseridir. Yapılan çalışmalarda DMH’in gastrointestinal sistemde birikerek organların fonksiyonlarını bozduğu ve kansere yol açtığı görülmüştür. Zeolit, adsorbsiyon ve iyon değiştirme özelliği nedeniyle birçok alanda kullanılmakla birlikte özellikle tıpta kullanımı da yaygınlaşan aluminyum silikat yapısındaki bir volkanik mineraldir. Zeolitin en bilinen türü olan ve zararsız olduğu kanıtlanan klinoptilolitin, insanlara oral yolla verildiğinde kana geçmediği ve gastrointestinal sistemden feçes olarak atıldığı görülmüştür. Ayrıca insanlara oral yolla verilen klinoptilolitin, kanda çözünmeden gastrointestinal sistemden geçtiği görülmüştür Gereç ve Yöntem: Çalışmada sıçanlarda DMH ile oluşturulan kolon kanserine klinoptilolit ile beslenmenin etkileri araştırılmıştır. Deneyde kontrol, klinoptilolit, DMH ve klinoptilolit+DMH grubu olmak üzere 27 Wistar albino sıçan kullanılmıştır. 16 hafta boyunca DMH grubuna haftada bir DMH enjekte edilirken; klinoptilolit ve klinoptilolit+DMH grupları klinoptilolit ile beslenmiştir. Alınan tüm kolon örnekleri rutin preparasyon işlemlerinden geçirilerek, ışık mikroskobunda incelenmiştir. Ayrıca kolon kanserinde sık rastlanan Wnt-ß-katenin sinyal yoluna özgü ß-katenin antikoru ile immunhistokimyasal boyama yapılarak moleküler değişiklikler belirlenmiştir. Bulgular: DMH grubunda epitel hücrelerinin boylarında kısalma, nükleuslarında şekil değişiklikleri, kripta hücrelerinde büyüme, kan damarı ve bağ doku miktarında artış ve lökosit infiltrasyonu gözlenmiştir. DMH+Klinoptilolit uygulanmasının bu değişiklikleri azalttığı saptanmıştır. ß-katenin reaksiyon şiddeti sırasıyla DMH> klinoptilolit> kontrol > DMH + Klinoptilolit olarak belirlenmiştir. Sonuç: Klinoptilolitin, hücre proliferasyonunu azaltarak DMH’nin olası toksik etkilerini azalttığı düşünülmektedir.

Anahtar Kelimeler:

Klinoptilolit, DMH, Kolon kanseri, histokimya

THE EFFECTS OF ZEOLITE SUPPORTED NUTRITION ON CANCER DEVELOPMENT IN THE 1,2 DYMETHYLHYDRASIN INDUCED -COLON CANCER MODEL

Objective: Environmental pollution and nutrition with plants and animals exposed to chemical substances such as 1,2-Dimethylhydrazine (DMH) causes many diseases. Colon cancer is one of them. DMH has been found to accumulate in the gastrointestinal tract, impairing the functions of its organs and causing cancer. Zeolite is a volcanic mineral which contains aluminum silicate, which is used in many areas due to its absorption and ion exchange capability, and which has had widespread medical use in recent years. Clinoptilolite, the most well-known species of zeolite, proved to be harmless, did not enter the blood when administered orally to humans and was excreted as a faeces from the gastrointestinal tract. In addition, clinoptilolite administered orally to humans passes through the gastrointestinal tract without dissolving in the blood. Material and Method: The aim of this study was to investigate the effects of clinoptilolite nutrition on colon cancer induced by DMH in rats. The rats were divided into four experimental groups which were control, clinoptilolite, DMH, and clinoptilolite+DMH using a total of 46 Wistar albino rats. Rats of DMH and clinoptilolite + DMH groups were injected withDMH subcutaneously once a week for 16 weeks at a dose of 20 mg/kg and also clinoptilolite and clinoptilolite+DMH groups were fed a diet with 50% clinoptilolite for eight months. Results: Molecular changes were examined by immunohistochemical staining in colon tissue samples with ß-catenin antibody specific for Wnt-ß-catenin signaling pathway, which is common in colon cancer. The reaction intensity of ß-catenin was determined as DMH> clinoptilolite control> DMH + clinoptilolite, respectively. Conclusion: We may think that clinoptilolite reduces the potential toxic effects of DMH by decreasing cell proliferation.

Keywords:

Clinoptilolite, DMH, Colon cancer, histochemistry,

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